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Rabbit polyclonal to Heterogeneous Nuclear Ribonucleoprotein (A1-like)
Predicted to work with:
Mouse, Rat, Cow, Dog, Pig, Chimpanzee
A region within synthetic peptide: MSKSASPKEP EQLRKLFIGG LSFETTDESL RSHFEQWGTL TDCVVMRDPN, corresponding to N terminal amino acids 1-50 of Human Heterogeneous Nuclear Ribonucleoprotein (A1-like)
WB: Jurkat cell lysate.
IHC-P: Human stomach tissue.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Preservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
Protein A purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
Titre using peptide based assay: 1/312500.
Use a concentration of 1.25 µg/ml. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use a concentration of 4 - 8 µg/ml.
The function remains unknown.
Western blot - Anti-Heterogeneous Nuclear Ribonucleoprotein (A1-like) antibody (ab50966)
All lanes : Anti-Heterogeneous Nuclear Ribonucleoprotein (A1-like) antibody (ab50966) at 1.25 µg/ml Lane 1 : MW marker Lane 2 : Jurkat cell lysate Lysates/proteins at 10 µg per lane. Secondary Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution Predicted band size: 34 kDa Observed band size: 34 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heterogeneous Nuclear Ribonucleoprotein (A1-like) antibody (ab50966)
ab50966, at a concentration of 8µg/ml, staining Heterogeneous Nuclear Ribonucleoprotein (A1-like) in paraffin embedded human stomach tissue sections. The positive cells (indicated by arrows) are epithelial cells of fundic glands.
has not yet been referenced specifically in any publications.
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