Product nameAnti-Heterogeneous Nuclear Ribonucleoprotein (A1-like) antibody [EPR13673-33]
See all Heterogeneous Nuclear Ribonucleoprotein (A1-like) primary antibodies
DescriptionRabbit monoclonal [EPR13673-33] to Heterogeneous Nuclear Ribonucleoprotein (A1-like)
Tested applicationsSuitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Heterogeneous Nuclear Ribonucleoprotein (A1-like) aa 250 to the C-terminus. The exact sequence is proprietary.
Database link: Q32P51
- Jurkat and K562 cell lysate. Human transitional cell carcinoma of bladder tissue. A431 and K562 cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab181337 in the following tested applications.
|WB||1/10000 - 1/50000. Predicted molecular weight: 34 kDa.|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
RelevanceThe function remains unknown.
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All lanes : Anti-Heterogeneous Nuclear Ribonucleoprotein (A1-like) antibody [EPR13673-33] (ab181337) at 1/10000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : K562 cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 34 kDa
Blocking/ Dilution buffer: 5% NFDM /TBST.
Immunohistochemical analysis of paraffin embedded Human transitional cell carcinoma of bladder tissue sections labeling Heterogeneous Nuclear Ribonucleoprotein (A1-like) using ab181337 at a 1/100 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunofluorescent analysis of paraformaldehyde fixed A431 cells labeling Heterogeneous Nuclear Ribonucleoprotein (A1-like) using ab181337 at a 1/250 dilution. A Goat anti rabbit IgG (Alexa Fluor®488) was used as the secondary at a 1/200 dilution.
Flow Cytometry analysis of K562 cells labeling Heterogeneous Nuclear Ribonucleoprotein (A1-like) using ab181337 at a 1/20 dilution (pink). Goat anti rabbit IgG (FITC) used as the secondary at a 1/150 dilution. Isotype control Rabbit monoclonal IgG (green). Cells were fixed in 2% paraformaldehyde.
ab181337 has not yet been referenced specifically in any publications.