Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13673] to Heterogeneous Nuclear Ribonucleoprotein (A1-like)
- Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
- Reacts with: Mouse, Rat, Human
Product nameAnti-Heterogeneous Nuclear Ribonucleoprotein (A1-like) antibody [EPR13673]
See all Heterogeneous Nuclear Ribonucleoprotein (A1-like) primary antibodies
DescriptionRabbit monoclonal [EPR13673] to Heterogeneous Nuclear Ribonucleoprotein (A1-like)
Tested applicationsSuitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Heterogeneous Nuclear Ribonucleoprotein (A1-like) aa 250 to the C-terminus. The exact sequence is proprietary.
Database link: Q32P51
- Jurkat, K562, A431 and 293 cell lysates; Human cervix squamous cell carcinoma tissue; K562 and 293 cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab180124 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000 - 1/10000. Predicted molecular weight: 34 kDa.|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
RelevanceThe function remains unknown.
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All lanes : Anti-Heterogeneous Nuclear Ribonucleoprotein (A1-like) antibody [EPR13673] (ab180124) at 1/20000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : K562 cell lysate
Lane 3 : A431 cell lysate
Lane 4 : 293 cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 34 kDa
Immunohistochemical analysis of paraffin-embedded Human cervix squamous cell carcinoma tissue labeling Heterogeneous Nuclear Ribonucleoprotein (A1-like) with ab180124 at 1/250 dilution, followed by Goat anti rabbit IgG(HRP) secondary and counterstained with Hematoxylin.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunofluorescent analysis of 293 (paraformaldehyde-fixed cells, 4%) labeling Heterogeneous Nuclear Ribonucleoprotein (A1-like) with ab180124 at 1/250 dilution, followed by Goat anti rabbit IgG(Dylight 488) at 1/250 dilution and counterstained with DAPI (blue).
Flow cytometric analysis of K562 cells (paraformaldehyde-fixed, 2%) labeling Heterogeneous Nuclear Ribonucleoprotein (A1-like) with ab180124 at 1/10 dilution or a rabbit IgG (negative), followed by secondary Goat anti rabbit IgG (FITC) at 1/75 dilution.
ab180124 has been referenced in 1 publication.
- Lim J et al. The histone variant H3.3 G34W substitution in giant cell tumor of the bone link chromatin and RNA processing. Sci Rep 7:13459 (2017). PubMed: 29044188