For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    hev-b5-elisa-kit-ab215423.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Immunology Immune System Diseases Allergens
Share by email

Hev b5 ELISA kit (ab215423)

  • Datasheet
  • SDS
  • Protocol Booklet
Submit a review Submit a question

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Hev b5 ELISA kit (ab215423) typical standard curve.

    Key features and details

    • Sensitivity: 0.5 µg/L
    • Range: 5 µg/L - 100 µg/L
    • Detection method: Colorimetric
    • Assay type: Sandwich (quantitative)

    Overview

    • Product name

      Hev b5 ELISA kit
    • Detection method

      Colorimetric
    • Precision

      Intra-assay
      Sample n Mean SD CV%
      Spike (low) 16 21µg/L 0.9 4.4%
      Spike (med) 16 31µg/L 0.8 2.5%
      Spike (high) 16 76µg/L 3.8 5.1%
      Inter-assay
      Sample n Mean SD CV%
      Spike (low) 5 5.7µg/L 0.3 5.2%
      Spike (med) 5 24µg/L 0.6 2.6%
      Spike (high) 5 86µg/L 4.7 5.4%
    • Assay type

      Sandwich (quantitative)
    • Sensitivity

      0.5 µg/L
    • Range

      5 µg/L - 100 µg/L
    • Recovery

      Sample specific recovery
      Sample type Average % Range
      Spike 99 0µg/L - 100µg/L
    • Assay time

      2h 00m
    • Assay duration

      Multiple steps standard assay
    • Product overview

      The Hev b5 ELISA (Enzyme-Linked Immunosorbent Assay) kit (ab215423)  is designed for the quantitative measurement of of Hev b5 in natural rubber latex products.


      This kit is based on the immunoenzymetric assay technique. Microplate wells are coated with anti-Hev b5 specific monoclonal capture antibody that during the first incubation binds Hev b5 molecules from the sample extract. After incubation, unbound material is removed by washing the wells. During the second incubation, horseradish peroxidase (HRP) labelled Hev b5-specific monoclonal detection antibody binds to a different epitope of Hev b5 molecules. As such, the two antibodies are a matched pair that forms a “sandwich” around the target Hev b5 allergen. After washing, HRP substrate is added and the intensity of the colour produced is directly proportional to the Hev b5 concentration of the sample.


       

    • Notes

      Products containing natural rubber latex (NRL) from the rubber tree Hevea brasiliensis are widely used due to the economical price and advantageous processing properties of natural rubber, although adverse reactions against a number of allergenic proteins contained in the NRL are well known and documented. 

      Latex allergens are proteins or polypeptides eluting from the manufactured products upon contact with skin, mucous membranes or other tissues. According to the current allergen nomenclature system maintained by the International Union of Immunological Societies (IUIS) under the WHO, thirteen latex allergens, which have been characterized at the primary structure level and are contained in the official allergen list, are named as Hev b1, Hev b 2, Hev b 3, Hev b 4, Hev b 5, Hev b 6 (6.01, 6.02, 6.03), Hev b 7, Hev b 8, Hev b 9, Hev b 10, Hev b 11, Hev b 12 and Hev b 13. Not all previously listed allergens are able to come through the production process. At present, four of these allergens (Hev b1, Hev b 3, Hev b 5 and Hev b 6.02) have been mostly detected in manufactured latex products. 

      Hev b5 ELISA (ab215423) kit is the first commercial quantitative test to measure Hev b5 immunologically in NRL products. By the use of specific monoclonal antibodies, sensitivity and specificity stay high even in the presence of other proteins or chemical substances derived from the manufacturing process of NRL products.

       

       

    • Platform

      Pre-coated microplate (12 x 8 well strips)

    Properties

    • Storage instructions

      Store at +4°C. Please refer to protocols.
    • Components 1 x 96 tests
      anti-Hev b5 coated microplate (12 x 8 wells) 1 unit
      Hev b 5 Assay Buffer 1 x 15ml
      Hev b 5 Calibrator A (0 µg/L) 1 x 500µl
      Hev b 5 Calibrator B (5 µg/L) 1 x 500µl
      Hev b 5 Calibrator C (10 µg/L) 1 x 500µl
      Hev b 5 Calibrator D (25 µg/L) 1 x 500µl
      Hev b 5 Calibrator E (50 µg/L) 1 x 500µl
      Hev b 5 Calibrator F (100 µg/L) 1 x 500µl
      Hev b 5 Control 1 x 500µl
      Hev b 5 Enzyme Conjugate 1 x 15ml
      HRP Substrate Solution 1 x 15ml
      PBS Wash Concentrate 1 x 50ml
      Stopping Solution 1 x 15ml
    • Research areas

      • Immunology
      • Immune System Diseases
      • Allergens
      • Cell Biology
      • Other Antibodies
      • Plant related targets
    • Relevance

      Liquid latex from the rubber tree, Hevea brasiliensis, is the source of natural rubber latex (NRL) and contains over 200 proteins; 14 of them have been identified as allergens. Only some allergens retain their allergenic properties through the manufacturing processes. The NRL allergens that have been shown to be clinically relevant to genuine NRL allergy, and present in the final NRL products with maintained allergenicity are Hev b1, Hev b3, Hev b5 and Hev b6.02.
    • Alternative names

      • Hev b 5
      • Major latex allergen

    Images

    • Hev b5 ELISA kit (ab215423) typical standard curve.
      Hev b5 ELISA kit (ab215423) typical standard curve.

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (0)

    Publishing research using ab215423? Please let us know so that we can cite the reference in this datasheet.

    ab215423 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab215423.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2021 Abcam plc. All rights reserved.