Anti-Hex antibody (ab34222)

Reviews (4)Q&A (1)References (3)

Overview

  • Product name

    Anti-Hex antibody
    See all Hex primary antibodies

  • Description

    Rabbit polyclonal to Hex

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ChIP, WB, IHC-FoFrmore details

  • Species reactivity

    Reacts with: Mouse
    Predicted to work with: Rat, Human, Pig

  • Immunogen

    Synthetic peptide corresponding to Human Hex aa 200 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab28465)

  • Positive control

    • This antibody gave a positive signal in Mouse Liver Tissue Lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab34222 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ChIP Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 30 kDa).Can be blocked with Human Hex peptide (ab28465).
IHC-FoFr 1/100.

Target

  • Function

    Recognizes the DNA sequence 5'-ATTAA-3'. Transcriptional repressor. May play a role in hematopoietic differentiation. Establishes anterior identity at two levels; acts early to enhance canonical WNT-signaling by repressing expression of TLE4, and acts later to inhibit NODAL-signaling by directly targeting NODAL.

  • Tissue specificity

    Liver and promyelocytic leukemia cell line HL60.

  • Sequence similarities

    Contains 1 homeobox DNA-binding domain.

  • Developmental stage

    Expressed during hematopoiesis.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Hematopoietically expressed homeobox antibody
    • Hematopoietically-expressed homeobox protein HHEX antibody
    • HEX antibody
    • HHEX antibody
    • HHEX_HUMAN antibody
    • HMPH antibody
    • Homeobox hematopoietically expressed antibody
    • Homeobox protein HEX antibody
    • Homeobox protein PRH antibody
    • HOX11L PEN antibody
    • PRH antibody
    • PRHX antibody
    • Proline rich homeodomain containing transcription factor antibody
    see all

Images

  • Western blot - Anti-Hex antibody (ab34222)
    Western blot - Anti-Hex antibody (ab34222)
    Anti-Hex antibody (ab34222) at 1 µg/ml + Liver (Mouse) Tissue Lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Performed under reducing conditions.

    Predicted band size: 30 kDa
    Observed band size: 37 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 42 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 30 seconds
  • Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Hex antibody (ab34222)
    Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Hex antibody (ab34222)This image is courtesy of an Abreview submitted by Dr Sophie Pezet

    ab34222 staining Hex in mouse brain tissue section by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue from 4% PFA perfused animals underwent overnight fixation in 4% paraformaldehyde, cryoprotected in 30% sucrose and cut using cryostat.The primary antibody was diluted, 1/100 (PBS + 0.3% Triton X100) and incubated with sample for 18 hours at 20°C. An Alexa Fluor®488 conjugated goat polyclonal to rabbit IgG at 1/1000 dilution, was used as secondary.

    See Abreview

References

This product has been referenced in:

  • Dyikanov DT  et al. Optimization of CRISPR/Cas9 Technology to Knock Out Genes of Interest in Aneuploid Cell Lines. Tissue Eng Part C Methods 25:168-175 (2019). Read more (PubMed: 30747044) »
  • Wadey KS  et al. Protein kinase CK2 inhibition suppresses neointima formation via a proline-rich homeodomain-dependent mechanism. Vascul Pharmacol 99:34-44 (2017). WB, IHC-P . Read more (PubMed: 28927755) »
See all 3 Publications for this product

Publishing research using ab34222? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-5 of 5 Abreviews or Q&A

IHC - Wholemount abreview for Anti-Hex antibody

 Poor
Abreviews
Application
IHC - Wholemount
Sample
Mouse Tissue (Wholemount immunostaining of E7.5 mouse embryo.)
Specification
Wholemount immunostaining of E7.5 mouse embryo.
Read More

Dr. Sophie Morgani

Verified customer

Submitted Aug 03 2018

Western blot abreview for Anti-Hex antibody - ChIP Grade

 Good
Abreviews
abreview image
Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (8%)
Sample
Human Cell lysate - whole cell (16HBE14o- cells and Caco2 cells)
Specification
16HBE14o- cells and Caco2 cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More

Abcam user community

Verified customer

Submitted Sep 12 2013

Question

I have received ab34222 and ab21624 and both vials did not contain the expected volume of product. ab34222 contained about 50ul instead of 200ul and ab21624 was about 70ul and should have been 100ul.

Read More
Answer

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab34222 and one vial of ab13537.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

Read More

ChIP abreview for Anti-Hex antibody - ChIP Grade

 Good
Abreviews
abreview image
Application
ChIP
Sample
Mouse Cell lysate - nuclear (haematopoietic progenitor cell line HPC-7)
Specification
haematopoietic progenitor cell line HPC-7
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 0.4% formaldehyde
Detection step
Real-time PCR
Positive control
region known to bind this transcription factor
Negative control
region known to be devoid of this transcription factor
Read More

Abcam user community

Verified customer

Submitted Jan 12 2010

Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Hex antibody - ChIP Grade

 Good
Abreviews
abreview image
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Brain sections)
Specification
Brain sections
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
No
Read More

Dr. Sophie Pezet

Verified customer

Submitted Jun 12 2009

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