Anti-Hex antibody (ab34222)
- Datasheet
- References (3)
- Protocols
Overview
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Product name
Anti-Hex antibody
See all Hex primary antibodies -
Description
Rabbit polyclonal to Hex -
Host species
Rabbit -
Tested applications
Suitable for: ChIP, WB, IHC-FoFrmore details -
Species reactivity
Reacts with: Mouse
Predicted to work with: Rat, Human, Pig -
Immunogen
Synthetic peptide corresponding to Human Hex aa 200 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab28465) -
Positive control
- This antibody gave a positive signal in Mouse Liver Tissue Lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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ChIP Related Products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab34222 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ChIP | Use at an assay dependent concentration. | |
WB | Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 30 kDa).Can be blocked with Human Hex peptide (ab28465). | |
IHC-FoFr | 1/100. |
Target
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Function
Recognizes the DNA sequence 5'-ATTAA-3'. Transcriptional repressor. May play a role in hematopoietic differentiation. Establishes anterior identity at two levels; acts early to enhance canonical WNT-signaling by repressing expression of TLE4, and acts later to inhibit NODAL-signaling by directly targeting NODAL. -
Tissue specificity
Liver and promyelocytic leukemia cell line HL60. -
Sequence similarities
Contains 1 homeobox DNA-binding domain. -
Developmental stage
Expressed during hematopoiesis. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 3087 Human
- Entrez Gene: 15242 Mouse
- Entrez Gene: 397232 Pig
- Entrez Gene: 79237 Rat
- Omim: 604420 Human
- SwissProt: Q03014 Human
- SwissProt: P43120 Mouse
- Unigene: 118651 Human
see all -
Alternative names
- Hematopoietically expressed homeobox antibody
- Hematopoietically-expressed homeobox protein HHEX antibody
- HEX antibody
see all
Images
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Anti-Hex antibody (ab34222) at 1 µg/ml + Liver (Mouse) Tissue Lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Performed under reducing conditions.
Predicted band size: 30 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
Additional bands at: 42 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds -
Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Hex antibody (ab34222)This image is courtesy of an Abreview submitted by Dr Sophie Pezet
ab34222 staining Hex in mouse brain tissue section by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue from 4% PFA perfused animals underwent overnight fixation in 4% paraformaldehyde, cryoprotected in 30% sucrose and cut using cryostat.The primary antibody was diluted, 1/100 (PBS + 0.3% Triton X100) and incubated with sample for 18 hours at 20°C. An Alexa Fluor®488 conjugated goat polyclonal to rabbit IgG at 1/1000 dilution, was used as secondary.
Datasheets and documents
References
This product has been referenced in:
- Dyikanov DT et al. Optimization of CRISPR/Cas9 Technology to Knock Out Genes of Interest in Aneuploid Cell Lines. Tissue Eng Part C Methods 25:168-175 (2019). Read more (PubMed: 30747044) »
- Wadey KS et al. Protein kinase CK2 inhibition suppresses neointima formation via a proline-rich homeodomain-dependent mechanism. Vascul Pharmacol 99:34-44 (2017). WB, IHC-P . Read more (PubMed: 28927755) »