Anti-Hex antibody (ab34222)

Reviews (4)Q&A (1)References (3)
Western blot - Anti-Hex antibody (ab34222)

    Key features and details

    • Rabbit polyclonal to Hex
    • Suitable for: WB
    • Reacts with: Mouse
    • Isotype: IgG

    Overview

    • Product name

      Anti-Hex antibody
      See all Hex primary antibodies

    • Description

      Rabbit polyclonal to Hex

    • Host species

      Rabbit

    • Tested applications

      Suitable for: WBmore details

    • Species reactivity

      Reacts with: Mouse
      Predicted to work with: Rat, Human, Pig

    • Immunogen

      Synthetic peptide corresponding to Human Hex aa 200 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
      (Peptide available as ab28465)

    • Positive control

      • This antibody gave a positive signal in Mouse Liver Tissue Lysate.

    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab34222 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB (1)
    Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 30 kDa).
    Notes
    WB
    Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 30 kDa).

    Target

    • Function

      Recognizes the DNA sequence 5'-ATTAA-3'. Transcriptional repressor. May play a role in hematopoietic differentiation. Establishes anterior identity at two levels; acts early to enhance canonical WNT-signaling by repressing expression of TLE4, and acts later to inhibit NODAL-signaling by directly targeting NODAL.

    • Tissue specificity

      Liver and promyelocytic leukemia cell line HL60.

    • Sequence similarities

      Contains 1 homeobox DNA-binding domain.

    • Developmental stage

      Expressed during hematopoiesis.

    • Cellular localization

      Nucleus.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • Hematopoietically expressed homeobox antibody
      • Hematopoietically-expressed homeobox protein HHEX antibody
      • HEX antibody
      • HHEX antibody
      • HHEX_HUMAN antibody
      • HMPH antibody
      • Homeobox hematopoietically expressed antibody
      • Homeobox protein HEX antibody
      • Homeobox protein PRH antibody
      • HOX11L PEN antibody
      • PRH antibody
      • PRHX antibody
      • Proline rich homeodomain containing transcription factor antibody
      see all

    Images

    • Western blot - Anti-Hex antibody (ab34222)
      Western blot - Anti-Hex antibody (ab34222)
      Anti-Hex antibody (ab34222) at 1 µg/ml + Liver (Mouse) Tissue Lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

      Performed under reducing conditions.

      Predicted band size: 30 kDa
      Observed band size: 37 kDa why is the actual band size different from the predicted?
      Additional bands at: 42 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 30 seconds

    References (3)

    Publishing research using ab34222? Please let us know so that we can cite the reference in this datasheet.

    ab34222 has been referenced in 3 publications.

    • Dyikanov DT  et al. Optimization of CRISPR/Cas9 Technology to Knock Out Genes of Interest in Aneuploid Cell Lines. Tissue Eng Part C Methods 25:168-175 (2019). PubMed: 30747044
    • Wadey KS  et al. Protein kinase CK2 inhibition suppresses neointima formation via a proline-rich homeodomain-dependent mechanism. Vascul Pharmacol 99:34-44 (2017). WB, IHC-P . PubMed: 28927755
    • Stepanova V  et al. Urokinase-type Plasminogen Activator (uPA) Promotes Angiogenesis by Attenuating Proline-rich Homeodomain Protein (PRH) Transcription Factor Activity and De-repressing Vascular Endothelial Growth Factor (VEGF) Receptor Expression. J Biol Chem 291:15029-45 (2016). Mouse . PubMed: 27151212

    Customer reviews and Q&As

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    1-5 of 5 Abreviews or Q&A

    IHC - Wholemount abreview for Anti-Hex antibody

     Poor
    Abreviews
    Application
    IHC - Wholemount
    Sample
    Mouse Tissue (Wholemount immunostaining of E7.5 mouse embryo.)
    Specification
    Wholemount immunostaining of E7.5 mouse embryo.
    Read More

    DR. Sophie Morgani

    Verified customer

    Submitted Aug 03 2018

    Western blot abreview for Anti-Hex antibody - ChIP Grade

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    30 µg
    Gel Running Conditions
    Reduced Denaturing (8%)
    Sample
    Human Cell lysate - whole cell (16HBE14o- cells and Caco2 cells)
    Specification
    16HBE14o- cells and Caco2 cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Abcam user community

    Verified customer

    Submitted Sep 12 2013

    ChIP abreview for Anti-Hex antibody - ChIP Grade

     Good
    Abreviews
    abreview image
    Application
    ChIP
    Sample
    Mouse Cell lysate - nuclear (haematopoietic progenitor cell line HPC-7)
    Specification
    haematopoietic progenitor cell line HPC-7
    Type
    Cross-linking (X-ChIP)
    Duration of cross-linking step: 10 minute(s) and 0 second(s)
    Specification of the cross-linking agent: 0.4% formaldehyde
    Detection step
    Real-time PCR
    Positive control
    region known to bind this transcription factor
    Negative control
    region known to be devoid of this transcription factor
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 12 2010

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Hex antibody - ChIP Grade

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Mouse Tissue sections (Brain sections)
    Specification
    Brain sections
    Fixative
    Paraformaldehyde
    Antigen retrieval step
    None
    Permeabilization
    No
    Read More

    DR. Sophie Pezet

    Verified customer

    Submitted Jun 12 2009

    Question

    I have received ab34222 and ab21624 and both vials did not contain the expected volume of product. ab34222 contained about 50ul instead of 200ul and ab21624 was about 70ul and should have been 100ul.

    Read More
    Answer

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab34222 and one vial of ab13537.

    To check the status of the order please contact our Customer Service team and reference this number.

    Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

    I wish you the best of luck with your research.

    Read More

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