Anti-Hex antibody (ab34222)
Key features and details
- Rabbit polyclonal to Hex
- Suitable for: ChIP, WB, IHC-FoFr
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-Hex antibody
See all Hex primary antibodies -
Description
Rabbit polyclonal to Hex -
Host species
Rabbit -
Tested applications
Suitable for: ChIP, WB, IHC-FoFrmore details -
Species reactivity
Reacts with: Mouse
Predicted to work with: Rat, Human, Pig
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Immunogen
Synthetic peptide corresponding to Human Hex aa 200 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab28465) -
Positive control
- This antibody gave a positive signal in Mouse Liver Tissue Lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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ChIP Related Products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab34222 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ChIP | Use at an assay dependent concentration. | |
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 30 kDa).Can be blocked with Human Hex peptide (ab28465). | |
| IHC-FoFr | 1/100. |
Target
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Function
Recognizes the DNA sequence 5'-ATTAA-3'. Transcriptional repressor. May play a role in hematopoietic differentiation. Establishes anterior identity at two levels; acts early to enhance canonical WNT-signaling by repressing expression of TLE4, and acts later to inhibit NODAL-signaling by directly targeting NODAL. -
Tissue specificity
Liver and promyelocytic leukemia cell line HL60. -
Sequence similarities
Contains 1 homeobox DNA-binding domain. -
Developmental stage
Expressed during hematopoiesis. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 3087 Human
- Entrez Gene: 15242 Mouse
- Entrez Gene: 397232 Pig
- Entrez Gene: 79237 Rat
- Omim: 604420 Human
- SwissProt: Q03014 Human
- SwissProt: P43120 Mouse
- Unigene: 118651 Human
see all -
Alternative names
- Hematopoietically expressed homeobox antibody
- Hematopoietically-expressed homeobox protein HHEX antibody
- HEX antibody
see all
Images
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Western blot - Anti-Hex antibody (ab34222)Anti-Hex antibody (ab34222) at 1 µg/ml + Liver (Mouse) Tissue Lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Performed under reducing conditions.
Predicted band size: 30 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
Additional bands at: 42 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds -
Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Hex antibody (ab34222)This image is courtesy of an Abreview submitted by Dr Sophie Pezetab34222 staining Hex in mouse brain tissue section by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue from 4% PFA perfused animals underwent overnight fixation in 4% paraformaldehyde, cryoprotected in 30% sucrose and cut using cryostat.The primary antibody was diluted, 1/100 (PBS + 0.3% Triton X100) and incubated with sample for 18 hours at 20°C. An Alexa Fluor®488 conjugated goat polyclonal to rabbit IgG at 1/1000 dilution, was used as secondary.
Protocols
Datasheets and documents
References (3)
ab34222 has been referenced in 3 publications.
- Dyikanov DT et al. Optimization of CRISPR/Cas9 Technology to Knock Out Genes of Interest in Aneuploid Cell Lines. Tissue Eng Part C Methods 25:168-175 (2019). PubMed: 30747044
- Wadey KS et al. Protein kinase CK2 inhibition suppresses neointima formation via a proline-rich homeodomain-dependent mechanism. Vascul Pharmacol 99:34-44 (2017). WB, IHC-P . PubMed: 28927755
- Stepanova V et al. Urokinase-type Plasminogen Activator (uPA) Promotes Angiogenesis by Attenuating Proline-rich Homeodomain Protein (PRH) Transcription Factor Activity and De-repressing Vascular Endothelial Growth Factor (VEGF) Receptor Expression. J Biol Chem 291:15029-45 (2016). Mouse . PubMed: 27151212
