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    hex-antibody-hhe5i261-ab50836.pdf

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Epigenetics and Nuclear Signaling Transcription Domain Families Developmental Families Other
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Anti-Hex antibody [HHE5I261] (ab50836)

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Western blot - Anti-Hex antibody [HHE5I261] (ab50836)

    Key features and details

    • Mouse monoclonal [HHE5I261] to Hex
    • Suitable for: WB
    • Reacts with: Recombinant fragment
    • Isotype: IgG1

    You may also be interested in

    Secondary
    Product image
    Goat Anti-Mouse IgG H&L (HRP) (ab205719)

    View more associated products

    Overview

    • Product name

      Anti-Hex antibody [HHE5I261]
      See all Hex primary antibodies
    • Description

      Mouse monoclonal [HHE5I261] to Hex
    • Host species

      Mouse
    • Specificity

      This antibody reacts with Hex.
    • Tested Applications & Species

      Application Species
      WB
      Recombinant fragment
      See all applications and species data
    • Immunogen

      Hex recombinant fragment (Human), containing 50-200 AAs from near the N-terminal.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
    • Storage buffer

      pH: 7.4
      Preservative: 0.05% Sodium azide
      Constituents: BSA, 1% PBS
    • Concentration information loading...
    • Purity

      Protein G purified
    • Purification notes

      Protein G purified from culture of supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (~95%) fetal bovine serum, filtered through a 0.22µm membrane.
    • Clonality

      Monoclonal
    • Clone number

      HHE5I261
    • Isotype

      IgG1
    • Research areas

      • Epigenetics and Nuclear Signaling
      • Transcription
      • Domain Families
      • Developmental Families
      • Other
      • Stem Cells
      • Lineage Markers
      • Mesoderm
      • Developmental Biology
      • Embryogenesis
      • Axis formation and symmetry
      • Developmental Biology
      • Lineage specification
      • Mesoderm

    Associated products

    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Isotype control

      • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Recombinant Protein

      • Recombinant Human Hex protein (ab134846)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab50836 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    WB
    Recombinant fragment
    All applications
    Human
    Application Abreviews Notes
    WB
    Use at an assay dependent concentration. Detects a band of approximately 43 kDa.
    Notes
    WB
    Use at an assay dependent concentration. Detects a band of approximately 43 kDa.

    Target

    • Function

      Recognizes the DNA sequence 5'-ATTAA-3'. Transcriptional repressor. May play a role in hematopoietic differentiation. Establishes anterior identity at two levels; acts early to enhance canonical WNT-signaling by repressing expression of TLE4, and acts later to inhibit NODAL-signaling by directly targeting NODAL.
    • Tissue specificity

      Liver and promyelocytic leukemia cell line HL60.
    • Sequence similarities

      Contains 1 homeobox DNA-binding domain.
    • Developmental stage

      Expressed during hematopoiesis.
    • Cellular localization

      Nucleus.
    • Target information above from: UniProt accession Q03014 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 3087 Human
      • Omim: 604420 Human
      • SwissProt: Q03014 Human
      • Unigene: 118651 Human
      • Alternative names

        • Hematopoietically expressed homeobox antibody
        • Hematopoietically-expressed homeobox protein HHEX antibody
        • HEX antibody
        • HHEX antibody
        • HHEX_HUMAN antibody
        • HMPH antibody
        • Homeobox hematopoietically expressed antibody
        • Homeobox protein HEX antibody
        • Homeobox protein PRH antibody
        • HOX11L PEN antibody
        • PRH antibody
        • PRHX antibody
        • Proline rich homeodomain containing transcription factor antibody
        see all

      Images

      • Western blot - Anti-Hex antibody [HHE5I261] (ab50836)
        Western blot - Anti-Hex antibody [HHE5I261] (ab50836)
        Anti-Hex antibody [HHE5I261] (ab50836)
        Observed band size: 43 kDa
        why is the actual band size different from the predicted?



        This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (0)

      Publishing research using ab50836? Please let us know so that we can cite the reference in this datasheet.

      ab50836 has not yet been referenced specifically in any publications.

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