Anti-Hex antibody [HHE5I261] (ab50836)
- Datasheet
- References
- Protocols
Overview
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Product nameAnti-Hex antibody [HHE5I261]
See all Hex primary antibodies -
DescriptionMouse monoclonal [HHE5I261] to Hex
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Host speciesMouse
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SpecificityThis antibody reacts with Hex.
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Tested applicationsSuitable for: WB, ELISAmore details
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Species reactivityReacts with: Recombinant fragment
Predicted to work with: Human
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Immunogen
Hex recombinant fragment (Human), containing 50-200 AAs from near the N-terminal.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
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Storage bufferPreservative: 0.05% Sodium azide
Constituents: BSA, 1% PBS -
Concentration information loading... -
PurityProtein G purified
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Purification notesProtein G purified from culture of supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (~95%) fetal bovine serum, filtered through a 0.22µm membrane.
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ClonalityMonoclonal
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Clone numberHHE5I261
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IsotypeIgG1
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab50836 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use at an assay dependent dilution. Detects a band of approximately 43 kDa. | |
| ELISA | Use at an assay dependent dilution. |
Target
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FunctionRecognizes the DNA sequence 5'-ATTAA-3'. Transcriptional repressor. May play a role in hematopoietic differentiation. Establishes anterior identity at two levels; acts early to enhance canonical WNT-signaling by repressing expression of TLE4, and acts later to inhibit NODAL-signaling by directly targeting NODAL.
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Tissue specificityLiver and promyelocytic leukemia cell line HL60.
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Sequence similaritiesContains 1 homeobox DNA-binding domain.
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Developmental stageExpressed during hematopoiesis.
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Cellular localizationNucleus.
- Information by UniProt
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Database links
- Entrez Gene: 3087 Human
- Omim: 604420 Human
- SwissProt: Q03014 Human
- Unigene: 118651 Human
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Alternative names
- Hematopoietically expressed homeobox antibody
- Hematopoietically-expressed homeobox protein HHEX antibody
- HEX antibody
see all
Images
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![Western blot - Anti-Hex antibody [HHE5I261] (ab50836)](https://www.abcam.com/ps/products/50/ab50836/Images/ab50836.jpg)
Western blot - Anti-Hex antibody [HHE5I261] (ab50836)Anti-Hex antibody [HHE5I261] (ab50836)
Observed band size: 43 kDa why is the actual band size different from the predicted?This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
Protocols
Datasheets and documents
References
ab50836 has not yet been referenced specifically in any publications.
