Anti-Hex antibody [HHE5I261] (ab50836)
![Western blot - Anti-Hex antibody [HHE5I261] (ab50836)](https://www.abcam.com/ps/products/50/ab50836/Images/ab50836-62537-anti-hex-antibody-hhe5i261-western-blot.jpg "Western blot - Anti-Hex antibody [HHE5I261] (ab50836)")
Key features and details
- Mouse monoclonal [HHE5I261] to Hex
- Suitable for: WB
- Reacts with: Recombinant fragment
- Isotype: IgG1
Overview
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Product name
Anti-Hex antibody [HHE5I261]
See all Hex primary antibodies -
Description
Mouse monoclonal [HHE5I261] to Hex -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Recombinant fragment
Predicted to work with: Human
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Immunogen
Hex recombinant fragment (Human), containing 50-200 AAs from near the N-terminal.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: BSA, 1% PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Protein G purified from culture of supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (~95%) fetal bovine serum, filtered through a 0.22µm membrane. -
Clonality
Monoclonal -
Clone number
HHE5I261 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab50836 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use at an assay dependent concentration. Detects a band of approximately 43 kDa.
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| Notes |
|---|
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WB
Use at an assay dependent concentration. Detects a band of approximately 43 kDa. |
Target
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Function
Recognizes the DNA sequence 5'-ATTAA-3'. Transcriptional repressor. May play a role in hematopoietic differentiation. Establishes anterior identity at two levels; acts early to enhance canonical WNT-signaling by repressing expression of TLE4, and acts later to inhibit NODAL-signaling by directly targeting NODAL. -
Tissue specificity
Liver and promyelocytic leukemia cell line HL60. -
Sequence similarities
Contains 1 homeobox DNA-binding domain. -
Developmental stage
Expressed during hematopoiesis. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 3087 Human
- Omim: 604420 Human
- SwissProt: Q03014 Human
- Unigene: 118651 Human
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Alternative names
- Hematopoietically expressed homeobox antibody
- Hematopoietically-expressed homeobox protein HHEX antibody
- HEX antibody
see all
Images
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Western blot - Anti-Hex antibody [HHE5I261] (ab50836)Anti-Hex antibody [HHE5I261] (ab50836)
Observed band size: 43 kDa why is the actual band size different from the predicted?This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
Datasheets and documents
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Datasheet download
References (0)
ab50836 has not yet been referenced specifically in any publications.
