Anti-Hex antibody [OTI3E6] (ab117864)

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Key features and details

  • Mouse monoclonal [OTI3E6] to Hex
  • Suitable for: WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG2b

Overview

  • Product name

    Anti-Hex antibody [OTI3E6]
    See all Hex primary antibodies

  • Description

    Mouse monoclonal [OTI3E6] to Hex

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse

  • Immunogen

    Recombinant fragment corresponding to Human Hex aa 100-270. Produced in E.coli (NP_002720).
    Sequence:

    LYPFPRTVNDYTHALLRHDPLGKPLLWSPFLQRPLHKRKGGQVRFSNDQT IELEKKFETQKYLSPPERKRLAKMLQLSERQVKTWFQNRRAKWRRLKQEN PQSNKKEELESLDSSCDQRQDLPSEQNKGASLDSSQCSPSPASQEDLESE ISEDSDQEVDIEGDKSYFNAG


    Database link: Q03014

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: HEK-293T cells lysate transfected with pCMV6-ENTRY Hex cDNA; HepG2 and HT-29 cell lysates. ICC/IF: HT-29 cells

  • General notes

    Clone OTI3E6 (formerly 3E6).

Properties

Applications

Our Abpromise guarantee covers the use of ab117864 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 30 kDa.
ICC/IF 1/100.

Target

  • Function

    Recognizes the DNA sequence 5'-ATTAA-3'. Transcriptional repressor. May play a role in hematopoietic differentiation. Establishes anterior identity at two levels; acts early to enhance canonical WNT-signaling by repressing expression of TLE4, and acts later to inhibit NODAL-signaling by directly targeting NODAL.

  • Tissue specificity

    Liver and promyelocytic leukemia cell line HL60.

  • Sequence similarities

    Contains 1 homeobox DNA-binding domain.

  • Developmental stage

    Expressed during hematopoiesis.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    • Alternative names

      • Hematopoietically expressed homeobox antibody
      • Hematopoietically-expressed homeobox protein HHEX antibody
      • HEX antibody
      • HHEX antibody
      • HHEX_HUMAN antibody
      • HMPH antibody
      • Homeobox hematopoietically expressed antibody
      • Homeobox protein HEX antibody
      • Homeobox protein PRH antibody
      • HOX11L PEN antibody
      • PRH antibody
      • PRHX antibody
      • Proline rich homeodomain containing transcription factor antibody
      see all

    Images

    • Western blot - Anti-Hex antibody [OTI3E6] (ab117864)
      Western blot - Anti-Hex antibody [OTI3E6] (ab117864)
      All lanes : Anti-Hex antibody [OTI3E6] (ab117864) at 1/1000 dilution

      Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY control
      Lane 2 : HEK-293T cells lysate transfected with pCMV6-ENTRY Hex cDNA

      Lysates/proteins at 5 µg per lane.

      Predicted band size: 30 kDa

    • Western blot - Anti-Hex antibody [OTI3E6] (ab117864)
      Western blot - Anti-Hex antibody [OTI3E6] (ab117864)
      All lanes : Anti-Hex antibody [OTI3E6] (ab117864) at 1/1000 dilution

      Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) cell extract
      Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract
      Lane 3 : HT-29 (human colorectal adenocarcinoma cell line) cell extract
      Lane 4 : A549 (human lung carcinoma cell line) cell extract
      Lane 5 : COS-7 (african green monkey kidney fibroblast-like cell line) cell extract
      Lane 6 : Jurkat (human T cell leukemia cell line from peripheral blood) cell extract
      Lane 7 : MDCK (canine kidney cell line) cell extract
      Lane 8 : PC-12 (rat adrenal gland pheochromocytoma cell line) cell extract
      Lane 9 : MCF7 (human breast adenocarcinoma cell line) cell extract

      Lysates/proteins at 35 µg per lane.

      Predicted band size: 30 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-Hex antibody [OTI3E6] (ab117864)
      Immunocytochemistry/ Immunofluorescence - Anti-Hex antibody [OTI3E6] (ab117864)

      HT-29 (human colorectal adenocarcinoma cell line) cells stained for Hex (green) using ab117864 at 1/100 dilution in ICC/IF.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References

    Publishing research using ab117864? Please let us know so that we can cite the reference in this datasheet.

    ab117864 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Immunocytochemistry/ Immunofluorescence abreview for Anti-Hex antibody [3E6]

     Good
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (H9 embryonic stem cells)
    Specification
    H9 embryonic stem cells
    Blocking step
    Serum as blocking agent for 10 minute(s) · Concentration: 5% · Temperature: 4°C
    Fixative
    Paraformaldehyde
    Read More

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    Verified customer

    Submitted Oct 22 2014

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