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  1. Link

    hex-antibody-oti3e6-ab117864.pdf

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Epigenetics and Nuclear Signaling Transcription Domain Families Developmental Families Other
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Anti-Hex antibody [OTI3E6] (ab117864)

  • Datasheet
  • SDS
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Western blot - Anti-Hex antibody [OTI3E6] (ab117864)
  • Western blot - Anti-Hex antibody [OTI3E6] (ab117864)
  • Immunocytochemistry/ Immunofluorescence - Anti-Hex antibody [OTI3E6] (ab117864)

Key features and details

  • Mouse monoclonal [OTI3E6] to Hex
  • Suitable for: WB, ICC/IF
  • Reacts with: Dog, Human, African green monkey
  • Isotype: IgG2b

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Anti-Nanog antibody [NNG-811] (ab62734)

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Overview

  • Product name

    Anti-Hex antibody [OTI3E6]
    See all Hex primary antibodies
  • Description

    Mouse monoclonal [OTI3E6] to Hex
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Dog, Human, African green monkey
    Predicted to work with: Mouse
  • Immunogen

    Recombinant fragment corresponding to Human Hex aa 100 to the C-terminus. Produced in E.coli (NP_002720).
    Database link: Q03014

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • WB: HEK-293T cells lysate transfected with pCMV6-ENTRY Hex cDNA; HepG2 and HT-29 cell lysates. ICC/IF: HT-29 cells
  • General notes

    Clone OTI3E6 (formerly 3E6).

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 1% BSA, 50% Glycerol
  • Concentration information loading...
  • Purity

    Affinity purified
  • Purification notes

    Purified from cell culture supernatant by affinity chromatography.
  • Clonality

    Monoclonal
  • Clone number

    OTI3E6
  • Isotype

    IgG2b
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Developmental Families
    • Other
    • Stem Cells
    • Lineage Markers
    • Mesoderm
    • Developmental Biology
    • Embryogenesis
    • Axis formation and symmetry
    • Developmental Biology
    • Lineage specification
    • Mesoderm

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control (ab170192)
  • Positive Controls

    • Hep G2 whole cell lysate (ab166833)
  • Recombinant Protein

    • Recombinant Human Hex protein (ab134846)
  • Related Products

    • Recombinant Human Hex protein (ab134846)
    • Recombinant Human Hex protein (ab167885)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab117864 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000. Predicted molecular weight: 30 kDa.
ICC/IF (1)
1/100.
Notes
WB
1/1000. Predicted molecular weight: 30 kDa.
ICC/IF
1/100.

Target

  • Function

    Recognizes the DNA sequence 5'-ATTAA-3'. Transcriptional repressor. May play a role in hematopoietic differentiation. Establishes anterior identity at two levels; acts early to enhance canonical WNT-signaling by repressing expression of TLE4, and acts later to inhibit NODAL-signaling by directly targeting NODAL.
  • Tissue specificity

    Liver and promyelocytic leukemia cell line HL60.
  • Sequence similarities

    Contains 1 homeobox DNA-binding domain.
  • Developmental stage

    Expressed during hematopoiesis.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession Q03014 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3087 Human
    • Entrez Gene: 15242 Mouse
    • Omim: 604420 Human
    • SwissProt: Q03014 Human
    • SwissProt: P43120 Mouse
    • Unigene: 118651 Human
    • Unigene: 33896 Mouse
    • Alternative names

      • Hematopoietically expressed homeobox antibody
      • Hematopoietically-expressed homeobox protein HHEX antibody
      • HEX antibody
      • HHEX antibody
      • HHEX_HUMAN antibody
      • HMPH antibody
      • Homeobox hematopoietically expressed antibody
      • Homeobox protein HEX antibody
      • Homeobox protein PRH antibody
      • HOX11L PEN antibody
      • PRH antibody
      • PRHX antibody
      • Proline rich homeodomain containing transcription factor antibody
      see all

    Images

    • Western blot - Anti-Hex antibody [OTI3E6] (ab117864)
      Western blot - Anti-Hex antibody [OTI3E6] (ab117864)
      All lanes : Anti-Hex antibody [OTI3E6] (ab117864) at 1/1000 dilution

      Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY control
      Lane 2 : HEK-293T cells lysate transfected with pCMV6-ENTRY Hex cDNA

      Lysates/proteins at 5 µg per lane.

      Predicted band size: 30 kDa

    • Western blot - Anti-Hex antibody [OTI3E6] (ab117864)
      Western blot - Anti-Hex antibody [OTI3E6] (ab117864)
      All lanes : Anti-Hex antibody [OTI3E6] (ab117864) at 1/1000 dilution

      Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) cell extract
      Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract
      Lane 3 : HT-29 (human colorectal adenocarcinoma cell line) cell extract
      Lane 4 : A549 (human lung carcinoma cell line) cell extract
      Lane 5 : COS-7 (african green monkey kidney fibroblast-like cell line) cell extract
      Lane 6 : Jurkat (human T cell leukemia cell line from peripheral blood) cell extract
      Lane 7 : MDCK (canine kidney cell line) cell extract
      Lane 8 : PC-12 (rat adrenal gland pheochromocytoma cell line) cell extract
      Lane 9 : MCF7 (human breast adenocarcinoma cell line) cell extract

      Lysates/proteins at 35 µg per lane.

      Predicted band size: 30 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-Hex antibody [OTI3E6] (ab117864)
      Immunocytochemistry/ Immunofluorescence - Anti-Hex antibody [OTI3E6] (ab117864)

      HT-29 (human colorectal adenocarcinoma cell line) cells stained for Hex (green) using ab117864 at 1/100 dilution in ICC/IF.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab117864? Please let us know so that we can cite the reference in this datasheet.

    ab117864 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Hex antibody [3E6]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (H9 embryonic stem cells)
    Specification
    H9 embryonic stem cells
    Blocking step
    Serum as blocking agent for 10 minute(s) · Concentration: 5% · Temperature: 4°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Oct 22 2014

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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