Anti-Hex antibody [OTI3E6] (ab117864)
![Western blot - Anti-Hex antibody [OTI3E6] (ab117864)](https://www.abcam.com/ps/products/117/ab117864/Images/ab117864-129582-anti-hex-antibody-oti3e6-western-blot.jpg "Western blot - Anti-Hex antibody [OTI3E6] (ab117864)")
Key features and details
- Mouse monoclonal [OTI3E6] to Hex
- Suitable for: WB, ICC/IF
- Reacts with: Dog, Human, African green monkey
- Isotype: IgG2b
Overview
-
Product name
Anti-Hex antibody [OTI3E6]
See all Hex primary antibodies -
Description
Mouse monoclonal [OTI3E6] to Hex -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Dog, Human, African green monkey
Predicted to work with: Mouse
-
Immunogen
Recombinant fragment corresponding to Human Hex aa 100 to the C-terminus. Produced in E.coli (NP_002720).
Database link: Q03014 -
Positive control
- WB: HEK-293T cells lysate transfected with pCMV6-ENTRY Hex cDNA; HepG2 and HT-29 cell lysates. ICC/IF: HT-29 cells
-
General notes
Clone OTI3E6 (formerly 3E6).
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
Purified from cell culture supernatant by affinity chromatography. -
Clonality
Monoclonal -
Clone number
OTI3E6 -
Isotype
IgG2b -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab117864 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Predicted molecular weight: 30 kDa.
|
|
| ICC/IF | (1) |
1/100.
|
| Notes |
|---|
|
WB
1/1000. Predicted molecular weight: 30 kDa. |
|
ICC/IF
1/100. |
Target
-
Function
Recognizes the DNA sequence 5'-ATTAA-3'. Transcriptional repressor. May play a role in hematopoietic differentiation. Establishes anterior identity at two levels; acts early to enhance canonical WNT-signaling by repressing expression of TLE4, and acts later to inhibit NODAL-signaling by directly targeting NODAL. -
Tissue specificity
Liver and promyelocytic leukemia cell line HL60. -
Sequence similarities
Contains 1 homeobox DNA-binding domain. -
Developmental stage
Expressed during hematopoiesis. -
Cellular localization
Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 3087 Human
- Entrez Gene: 15242 Mouse
- Omim: 604420 Human
- SwissProt: Q03014 Human
- SwissProt: P43120 Mouse
- Unigene: 118651 Human
- Unigene: 33896 Mouse
-
Alternative names
- Hematopoietically expressed homeobox antibody
- Hematopoietically-expressed homeobox protein HHEX antibody
- HEX antibody
see all
Images
-
Western blot - Anti-Hex antibody [OTI3E6] (ab117864)All lanes : Anti-Hex antibody [OTI3E6] (ab117864) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY control
Lane 2 : HEK-293T cells lysate transfected with pCMV6-ENTRY Hex cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size: 30 kDa -
![Western blot - Anti-Hex antibody [OTI3E6] (ab117864)](https://www.abcam.com/ps/products/117/ab117864/Images/ab117864-129581-anti-hex-antibody-oti3e6-western-blot.jpg)
Western blot - Anti-Hex antibody [OTI3E6] (ab117864)All lanes : Anti-Hex antibody [OTI3E6] (ab117864) at 1/1000 dilution
Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) cell extract
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract
Lane 3 : HT-29 (human colorectal adenocarcinoma cell line) cell extract
Lane 4 : A549 (human lung carcinoma cell line) cell extract
Lane 5 : COS-7 (african green monkey kidney fibroblast-like cell line) cell extract
Lane 6 : Jurkat (human T cell leukemia cell line from peripheral blood) cell extract
Lane 7 : MDCK (canine kidney cell line) cell extract
Lane 8 : PC-12 (rat adrenal gland pheochromocytoma cell line) cell extract
Lane 9 : MCF7 (human breast adenocarcinoma cell line) cell extract
Lysates/proteins at 35 µg per lane.
Predicted band size: 30 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-Hex antibody [OTI3E6] (ab117864)](https://www.abcam.com/ps/products/117/ab117864/Images/ab117864-129580-anti-hex-antibody-oti3e6-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Hex antibody [OTI3E6] (ab117864)HT-29 (human colorectal adenocarcinoma cell line) cells stained for Hex (green) using ab117864 at 1/100 dilution in ICC/IF.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
Datasheet download
References (0)
ab117864 has not yet been referenced specifically in any publications.
