Key features and details
- Rabbit polyclonal to HEXIM1
- Suitable for: IP, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-HEXIM1 antibody
See all HEXIM1 primary antibodies
DescriptionRabbit polyclonal to HEXIM1
Tested applicationsSuitable for: IP, WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Cow
Synthetic peptide within Human HEXIM1 aa 175-225. The exact sequence is proprietary. NP_006451.1
Database link: O94992
- WB: HeLa whole cell lysate. IP: HeLa whole cell lysate. IHC-P: Human lung cancer tissue; mouse teratoma tissue.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab245494 was affinity purified using an epitope specific to HEXIM1 immobilized on solid support.
Our Abpromise guarantee covers the use of ab245494 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-10 µg/mg of lysate.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 41 kDa.|
|IHC-P||1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionTranscriptional regulator which functions as a general RNA polymerase II transcription inhibitor. In cooperation with 7SK snRNA sequesters P-TEFb in a large inactive 7SK snRNP complex preventing RNA polymerase II phosphorylation and subsequent transcriptional elongation. May also regulate NF-kappa-B, ESR1, NR3C1 and CIITA-dependent transcriptional activity.
Tissue specificityUbiquitously expressed with higher expression in placenta. HEXIM1 and HEXIM2 are differentially expressed. Expressed in endocrine tissues.
Sequence similaritiesBelongs to the HEXIM family.
DomainThe coiled-coil domain mediates oligomerization.
Cellular localizationNucleus. Cytoplasm. Binds alpha-importin and is mostly nuclear.
- Information by UniProt
- Cardiac lineage protein 1 antibody
- CLP 1 antibody
- CLP1 antibody
Formalin-fixed, paraffin-embedded mouse teratoma tissue stained for HEXIM1 using ab245494 at 1/200 dilution in immunohistochemical analysis. Detection: DAB staining.
Formalin-fixed, paraffin-embedded human lung cancer tissue stained for HEXIM1 using ab245494 at 1/1000 dilution in immunohistochemical analysis. Detection: DAB staining. Counterstained using hematoxylin (blue).
All lanes : Anti-HEXIM1 antibody (ab245494) at 0.04 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 41 kDa
Exposure time: 3 minutes
HEXIM1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab245494 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab245494 at 1 µg/ml.
Lane 1: ab245494 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 3 seconds.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab245494 has not yet been referenced specifically in any publications.