The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at 2-5 µg/mg of lysate.
Is unsuitable for WB.
Transcriptional regulator which functions as a general RNA polymerase II transcription inhibitor. In cooperation with 7SK snRNA sequesters P-TEFb in a large inactive 7SK snRNP complex preventing RNA polymerase II phosphorylation and subsequent transcriptional elongation.
Ubiquitously expressed with higher expression in testis. HEXIM1 and HEXIM2 are differentially expressed.
Detection of HEXIM2 by Western Blot of Immunprecipitate.
ab112041 at 1µg/ml staining HEXIM2 in HeLa whole cell lysate immunoprecipitated using ab112041 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 30 seconds.