Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: PBS, pH 7.4
Concentration information loading...
PurityProtein A purified
Purification notesPurified from serum by protein A chromatography.
Our Abpromise guarantee covers the use of ab93385 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionRNA exonuclease that binds to the 3'-end of histone mRNAs and degrades them, suggesting that it plays an essential role in histone mRNA decay after replication. A 2' and 3'-hydroxyl groups at the last nucleotide of the histone 3'-end is required for efficient degradation of RNA substrates. Also able to degrade the 3'-overhangs of short interfering RNAs (siRNAs) in vitro, suggesting a possible role as regulator of RNA interference (RNAi). Requires for binding the 5'-ACCCA-3' sequence present in stem-loop structure. Able to bind other mRNAs. Required for 5.8S rRNA 3'-end processing. Also binds to 5.8s ribosomal RNA. Binds with high affinity to the stem-loop structure of replication-dependent histone pre-mRNAs.
Sequence similaritiesContains 1 exonuclease domain.
Contains 1 SAP domain.
DomainThe SAP domain is necessary for binding to the stem-loop structure of histone mRNAs and to form the ternary complex with SLBP, but not for 3'-end histone mRNA exonuclease activity.
Cellular localizationCytoplasm. Nucleus. Nucleus > nucleolus.
- Information by UniProt
- 3' 5' exonuclease ERI 1 antibody
- 3' 5' exonuclease ERI1 antibody
- 3' EXO antibody
ab93385 has not yet been referenced specifically in any publications.