Product nameAnti-Hexokinase 1 antibody
See all Hexokinase 1 primary antibodies
DescriptionRabbit polyclonal to Hexokinase 1
Tested applicationsSuitable for: ELISA, WB, ICC/IF, IHC-Frmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide derived from an internal sequence of human Hexokinase 1. [Swiss-Prot P19367]
- Extracts from HeLa cells
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Our Abpromise guarantee covers the use of ab65069 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 102 kDa (predicted molecular weight: 102 kDa).|
|ICC/IF||1/250. (see Abreview)|
|IHC-Fr||1/200 - 1/250. (see Abreviews)|
Tissue specificityIsoform 2 is erythrocyte specific. Isoform 3 and isoform 4 are testis-specific.
PathwayCarbohydrate metabolism; hexose metabolism.
Involvement in diseaseHexokinase deficiency
Neuropathy, hereditary motor and sensory, Russe type
Sequence similaritiesBelongs to the hexokinase family.
Contains 2 hexokinase domains.
DomainThe N- and C-terminal halves of this hexokinase show extensive sequence similarity to each other. The catalytic activity is associated with the C-terminus while regulatory function is associated with the N-terminus. Each domain can bind a single glucose and Gluc-6-P molecule.
Cellular localizationMitochondrion outer membrane. Its hydrophobic N-terminal sequence may be involved in membrane binding.
- Information by UniProt
- BB404130 antibody
- Brain form hexokinase antibody
- dea antibody
All lanes : Anti-Hexokinase 1 antibody (ab65069) at 1/500 dilution
Lane 1 : extracts from HeLa cells
Lane 2 : extracts from HeLa cells with immunizing peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 102 kDa
Observed band size: 102 kDa
Additional bands at: 60 kDa. We are unsure as to the identity of these extra bands.
ab65069 staining Hexokinase 1 (green) in Rat brain tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with formaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 5% BSA for 2 hours at 25°C. Samples were incubated with primary antibody (1/250 in PBS-T) for 2 hours at 25°C. An Alexa Fluor®488-conjugated Goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody. Nuclei were stained by DAPI (blue).
ab65069 staining Hexokinase 1 in murine bone marrow leukocytes by Immunocytochemistry/ Immunofluorescence. The cells were fixed in methanol and then blocked using 5% serum for 2 hours at 25°C. Samples were then incubated with the primary antibody at 1/250 for 16 hours at 4°C. The secondary antibody used was a goat anti-rabbit IgG conjugated to Alexa Fluor® 488 (green) used at a 1/500 dilution. DAPI was used for staining nuclei.
This product has been referenced in:
- Yu L et al. Silencing the Girdin gene enhances radio-sensitivity of hepatocellular carcinoma via suppression of glycolytic metabolism. J Exp Clin Cancer Res 36:110 (2017). Read more (PubMed: 28810896) »
- Tegnebratt T et al. Evaluation of efficacy of a new MEK inhibitor, RO4987655, in human tumor xenografts by [(18)F] FDG-PET imaging combined with proteomic approaches. EJNMMI Res 4:34 (2014). IHC . Read more (PubMed: 26116108) »