Recombinant
RabMAb

Recombinant Anti-Hexokinase 1 antibody [EPR10134(B)] - BSA and Azide free (ab233837)

Overview

  • Product name

    Anti-Hexokinase 1 antibody [EPR10134(B)] - BSA and Azide free
    See all Hexokinase 1 primary antibodies
  • Description

    Rabbit monoclonal [EPR10134(B)] to Hexokinase 1 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cyt, ICC/IF, IHC-Pmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Hexokinase 1 aa 100-200 (internal sequence). The exact sequence is proprietary.

  • Positive control

    • MCF7 whole cell lysate (ab3871) can be used as a positive control in WB. IHC-P: Human kidney tissue.
  • General notes

    The formulation and the concentration of this product is compatible for metal-conjugation for mass cytometry (CyTOF®). 

    ab233837 is a PBS-only buffer format of ab150423. Please refer to ab150423 for recommended dilutions, protocols, and image data.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab233837 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 102 kDa.
Flow Cyt Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

ICC/IF Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Tissue specificity

      Isoform 2 is erythrocyte specific. Isoform 3 and isoform 4 are testis-specific.
    • Pathway

      Carbohydrate metabolism; hexose metabolism.
    • Involvement in disease

      Hexokinase deficiency
      Neuropathy, hereditary motor and sensory, Russe type
    • Sequence similarities

      Belongs to the hexokinase family.
      Contains 2 hexokinase domains.
    • Domain

      The N- and C-terminal halves of this hexokinase show extensive sequence similarity to each other. The catalytic activity is associated with the C-terminus while regulatory function is associated with the N-terminus. Each domain can bind a single glucose and Gluc-6-P molecule.
    • Cellular localization

      Mitochondrion outer membrane. Its hydrophobic N-terminal sequence may be involved in membrane binding.
    • Information by UniProt
    • Database links

    • Alternative names

      • BB404130 antibody
      • Brain form hexokinase antibody
      • dea antibody
      • DrHXK1 antibody
      • EC 2.7.1.1 antibody
      • Glycolytic enzyme antibody
      • HEXOKIN antibody
      • hexokinase I antibody
      • Hexokinase PI antibody
      • Hexokinase type I antibody
      • Hexokinase, tumor isozyme antibody
      • Hexokinase-1 antibody
      • Hexokinase-A antibody
      • HK I antibody
      • HK1 antibody
      • HK1 tb antibody
      • HK1 tc antibody
      • Hk1-s antibody
      • HK1-ta antibody
      • HK1-tb antibody
      • HK1-tc antibody
      • HKD antibody
      • HKI antibody
      • HMSNR antibody
      • HXK1 antibody
      • HXK1_HUMAN antibody
      • im:7148527 antibody
      • mHk1-s antibody
      • wu:fc09d08 antibody
      • wu:fc16e02 antibody
      • wu:fc21e02 antibody
      • wu:fq14b11 antibody
      • zgc:55790 antibody
      • zgc:77618 antibody
      see all

    Images

    • Overlay histogram showing K562 cells stained with ab150423 (red line). The cells were fixed with 80% methanol (5 min)/ and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab150423, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab150423).

    • Immunofluorescent analysis of 293 cells labelling Hexokinase 1 with ab150423 at 1/100 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab150423).

    • Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling Hexokinase 1 with ab150423 at 1/50 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab150423).

    References

    ab233837 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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