Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-Hexokinase 1 antibody [EPR10134(B)] - Mitochondrial Outer Membrane Marker (ab150423)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hexokinase 1 antibody [EPR10134(B)] - Mitochondrial Outer Membrane Marker (ab150423)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hexokinase 1 antibody [EPR10134(B)] - Mitochondrial Outer Membrane Marker (ab150423)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hexokinase 1 antibody [EPR10134(B)] - Mitochondrial Outer Membrane Marker (ab150423)
  • Western blot - Anti-Hexokinase 1 antibody [EPR10134(B)] - Mitochondrial Outer Membrane Marker (ab150423)
  • Flow Cytometry - Anti-Hexokinase 1 antibody [EPR10134(B)] - Mitochondrial Outer Membrane Marker (ab150423)
  • Immunocytochemistry - Anti-Hexokinase 1 antibody [EPR10134(B)] - Mitochondrial Outer Membrane Marker (ab150423)
  • Western blot - Anti-Hexokinase 1 antibody [EPR10134(B)] - Mitochondrial Outer Membrane Marker (ab150423)
  • Anti-Hexokinase 1 antibody [EPR10134(B)] - Mitochondrial Outer Membrane Marker (ab150423)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10134(B)] to Hexokinase 1 - Mitochondrial Outer Membrane Marker
  • Suitable for: WB, IHC-P, Flow Cyt, ICC
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Hexokinase 1 antibody [EPR10134(B)] - Mitochondrial Outer Membrane Marker
    See all Hexokinase 1 primary antibodies

  • Description

    Rabbit monoclonal [EPR10134(B)] to Hexokinase 1 - Mitochondrial Outer Membrane Marker

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt, ICCmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide corresponding to Human Hexokinase 1 aa 100-200 (internal sequence).

  • Positive control

    • WB: HEK-293T and MCF7 cells, human mouse and rat brain lysates; IHC: Human, mouse and rat kidney tissue; ICC: MCF7 lysate; Flow cyt: K-562 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab150423 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (4)
1/1000 - 1/10000. Predicted molecular weight: 102 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt
1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC
1/50.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 102 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt
1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC
1/50.

Target

  • Tissue specificity

    Isoform 2 is erythrocyte specific. Isoform 3 and isoform 4 are testis-specific.

  • Pathway

    Carbohydrate metabolism; hexose metabolism.

  • Involvement in disease

    Hexokinase deficiency
    Neuropathy, hereditary motor and sensory, Russe type

  • Sequence similarities

    Belongs to the hexokinase family.
    Contains 2 hexokinase domains.

  • Domain

    The N- and C-terminal halves of this hexokinase show extensive sequence similarity to each other. The catalytic activity is associated with the C-terminus while regulatory function is associated with the N-terminus. Each domain can bind a single glucose and Gluc-6-P molecule.

  • Cellular localization

    Mitochondrion outer membrane. Its hydrophobic N-terminal sequence may be involved in membrane binding.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • BB404130 antibody
    • Brain form hexokinase antibody
    • dea antibody
    • DrHXK1 antibody
    • EC 2.7.1.1 antibody
    • Glycolytic enzyme antibody
    • HEXOKIN antibody
    • hexokinase I antibody
    • Hexokinase PI antibody
    • Hexokinase type I antibody
    • Hexokinase, tumor isozyme antibody
    • Hexokinase-1 antibody
    • Hexokinase-A antibody
    • HK I antibody
    • HK1 antibody
    • HK1 tb antibody
    • HK1 tc antibody
    • Hk1-s antibody
    • HK1-ta antibody
    • HK1-tb antibody
    • HK1-tc antibody
    • HKD antibody
    • HKI antibody
    • HMSNR antibody
    • HXK1 antibody
    • HXK1_HUMAN antibody
    • im:7148527 antibody
    • mHk1-s antibody
    • wu:fc09d08 antibody
    • wu:fc16e02 antibody
    • wu:fc21e02 antibody
    • wu:fq14b11 antibody
    • zgc:55790 antibody
    • zgc:77618 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hexokinase 1 antibody [EPR10134(B)] - Mitochondrial Outer Membrane Marker (ab150423)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hexokinase 1 antibody [EPR10134(B)] - Mitochondrial Outer Membrane Marker (ab150423)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat kidney tissue sections labeling Hexokinase 1 with purified ab150423 at 1/50 dilution (4.14 µg/mL). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hexokinase 1 antibody [EPR10134(B)] - Mitochondrial Outer Membrane Marker (ab150423)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hexokinase 1 antibody [EPR10134(B)] - Mitochondrial Outer Membrane Marker (ab150423)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse kidney tissue sections labeling Hexokinase 1 with purified ab150423 at 1/50 dilution (4.14 µg/mL). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hexokinase 1 antibody [EPR10134(B)] - Mitochondrial Outer Membrane Marker (ab150423)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hexokinase 1 antibody [EPR10134(B)] - Mitochondrial Outer Membrane Marker (ab150423)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human kidney tissue sections labeling Hexokinase 1 with purified ab150423 at 1/50 dilution (4.14 µg/mL). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Western blot - Anti-Hexokinase 1 antibody [EPR10134(B)] - Mitochondrial Outer Membrane Marker (ab150423)
    Western blot - Anti-Hexokinase 1 antibody [EPR10134(B)] - Mitochondrial Outer Membrane Marker (ab150423)
    All lanes : Anti-Hexokinase 1 antibody [EPR10134(B)] - Mitochondrial Outer Membrane Marker (ab150423) at 1/1000 dilution (Purified)

    Lane 1 : Human brain lysate
    Lane 2 : Mouse brain lysate
    Lane 3 : Rat brain lysate
    Lane 4 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate
    Lane 5 : MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 102 kDa

  • Flow Cytometry - Anti-Hexokinase 1 antibody [EPR10134(B)] - Mitochondrial Outer Membrane Marker (ab150423)
    Flow Cytometry - Anti-Hexokinase 1 antibody [EPR10134(B)] - Mitochondrial Outer Membrane Marker (ab150423)
    Flow Cytometry analysis of K-562 (Human chronic myelogenous leukemia lymphoblast) cells labeling Hexokinase 1 with Purified ab150423 at 1:20 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor ® 488 ,ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
  • Immunocytochemistry - Anti-Hexokinase 1 antibody [EPR10134(B)] - Mitochondrial Outer Membrane Marker (ab150423)
    Immunocytochemistry - Anti-Hexokinase 1 antibody [EPR10134(B)] - Mitochondrial Outer Membrane Marker (ab150423)
    Immunocytochemistry analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling Hexokinase 1 with Purified ab150423 at 1:50 dilution (4.1 µg/ml). Cells were fixed in 100% Methanol and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488,ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
  • Western blot - Anti-Hexokinase 1 antibody [EPR10134(B)] - Mitochondrial Outer Membrane Marker (ab150423)
    Western blot - Anti-Hexokinase 1 antibody [EPR10134(B)] - Mitochondrial Outer Membrane Marker (ab150423)
    All lanes : Anti-Hexokinase 1 antibody [EPR10134(B)] - Mitochondrial Outer Membrane Marker (ab150423) at 1/1000 dilution

    Lane 1 : Wild-type HEK-293T cell lysate
    Lane 2 : HK1 knockout HeLa cell lysate
    Lane 3 : MCF7 cell lysate
    Lane 4 : HEPG2 cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 102 kDa
    Observed band size: 102 kDa



    Lanes 1-4: Merged signal (red and green). Green - ab150423 observed at 102 kDa. Red - loading control, ab8245 observed at 37 kDa.

    ab150423 Anti-Hexokinase 1 antibody [EPR10134(B)] - Mitochondrial was shown to specifically react with Hexokinase 1 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab267279 (knockout cell lysate ab257161) was used. Wild-type and Hexokinase 1 knockout samples were subjected to SDS-PAGE. ab150423 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

     

  • Anti-Hexokinase 1 antibody [EPR10134(B)] - Mitochondrial Outer Membrane Marker (ab150423)
    Anti-Hexokinase 1 antibody [EPR10134(B)] - Mitochondrial Outer Membrane Marker (ab150423)

References (6)

Publishing research using ab150423? Please let us know so that we can cite the reference in this datasheet.

ab150423 has been referenced in 6 publications.

  • Dijk SN  et al. Mitochondria as target to inhibit proliferation and induce apoptosis of cancer cells: the effects of doxycycline and gemcitabine. Sci Rep 10:4363 (2020). PubMed: 32152409
  • Yang HY  et al. Tankyrase Promotes Aerobic Glycolysis and Proliferation of Ovarian Cancer through Activation of Wnt/ß-Catenin Signaling. Biomed Res Int 2019:2686340 (2019). PubMed: 30915350
  • Xue D  et al. Quantitative proteomic analysis of sperm in unexplained recurrent pregnancy loss. Reprod Biol Endocrinol 17:52 (2019). PubMed: 31288842
  • Zawislak A  et al. Neuron-derived transthyretin modulates astrocytic glycolysis in hormone-independent manner. Oncotarget 8:106625-106638 (2017). PubMed: 29290976
  • Hauser DN  et al. Hexokinases link DJ-1 to the PINK1/parkin pathway. Mol Neurodegener 12:70 (2017). ICC/IF . PubMed: 28962651
  • Ben-Hail D  et al. Novel Compounds Targeting the Mitochondrial Protein VDAC1 Inhibit Apoptosis and Protect against Mitochondrial Dysfunction. J Biol Chem 291:24986-25003 (2016). PubMed: 27738100

Customer reviews and Q&As

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1-4 of 4 Abreviews

Western blot abreview for Anti-Hexokinase 1 antibody [EPR10134(B)] - Mitochondrial Outer Membrane Marker

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (brain)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
brain
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Dr. Yonatan Feuermann

Verified customer

Submitted Apr 12 2021

Western blot abreview for Anti-Hexokinase 1 antibody [EPR10134(B)] - Mitochondrial Outer Membrane Marker

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Rat Tissue lysate - whole (Retina)
Gel Running Conditions
Reduced Denaturing (12% gel)
Loading amount
20 µg
Specification
Retina
Blocking step
Licor Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 4°C
Read More

Abcam user community

Verified customer

Submitted Aug 01 2017

Western blot abreview for Anti-Hexokinase 1 antibody [EPR10134(B)] - Mitochondrial Outer Membrane Marker

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (hfRPE)
Gel Running Conditions
Reduced Denaturing (12% gel)
Loading amount
20 µg
Specification
hfRPE
Blocking step
Licor Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Read More

Abcam user community

Verified customer

Submitted Mar 20 2017

Western blot abreview for Anti-Hexokinase 1 antibody [EPR10134(B)] - Mitochondrial Outer Membrane Marker

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (Retina, RPE)
Gel Running Conditions
Reduced Denaturing (12% gel)
Loading amount
20 µg
Specification
Retina, RPE
Blocking step
Licor Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Read More

Abcam user community

Verified customer

Submitted Mar 20 2017

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