Anti-Hexokinase II antibody [3D3] (ab104836)
Key features and details
- Mouse monoclonal [3D3] to Hexokinase II
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Hexokinase II antibody [3D3]
See all Hexokinase II primary antibodies -
Description
Mouse monoclonal [3D3] to Hexokinase II -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-P, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human Hexokinase II.
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Positive control
- WB: Jurkat, HeLa and HEK293 cell lysates. IHC-P: Human esophagus and lung cancer tissues. Flow Cyt: K562 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.03% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
3D3 -
Isotype
IgG1 -
Research areas
Associated products
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Assay kits
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab104836 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (4) |
1/500 - 1/2000. Predicted molecular weight: 102 kDa.
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IHC-P | (3) |
1/200 - 1/1000.
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Flow Cyt |
Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Notes |
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WB
1/500 - 1/2000. Predicted molecular weight: 102 kDa. |
IHC-P
1/200 - 1/1000. |
Flow Cyt
Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
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Tissue specificity
Predominant hexokinase isozyme expressed in insulin-responsive tissues such as skeletal muscle. -
Pathway
Carbohydrate metabolism; hexose metabolism. -
Sequence similarities
Belongs to the hexokinase family.
Contains 2 hexokinase domains. -
Domain
The N- and C-terminal halves of this hexokinase show extensive sequence similarity to each other. The catalytic activity is associated with the C-terminus while regulatory function is associated with the N-terminus. Each domain can bind a single glucose and Gluc-6-P molecule. -
Cellular localization
Mitochondrion outer membrane. Its hydrophobic N-terminal sequence may be involved in membrane binding. - Information by UniProt
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Database links
- Entrez Gene: 3099 Human
- Omim: 601125 Human
- SwissProt: P52789 Human
- Unigene: 406266 Human
- Unigene: 591588 Human
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Alternative names
- DKFZp686M1669 antibody
- Hexokinase 2 antibody
- Hexokinase 2 muscle antibody
see all
Images
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All lanes : Anti-Hexokinase II antibody [3D3] (ab104836) at 1/500 dilution
Lane 1 : Wild-type HEK-293T cell lysate
Lane 2 : HK2 CRISPR/Cas9 edited HEK-293T cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : A549 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 102 kDa
Observed band size: 102 kDaLanes 1 - 4: Merged signal (red and green). Green - ab104836 observed at 102 kDa. Red - loading control ab181602 (Rabbit Anti-GAPDH antibody [EPR16891]) observed at 37kDa.
ab104836 was shown to react with Hexokinase II in wild-type HEK-293T cells in western blot. The band observed in HK2 CRISPR/Cas9 edited sample lane below 102 kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type and HK2 CRISPR/Cas9 edited HEK-293T cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab104836 and ab181602 (Rabbit Anti-GAPDH antibody [EPR16891]) overnight at 4°C at a 1 in 500 Dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Immunohistochemical analysis of human skin tissue, staining Hexokinase II with ab104836.
Tissue was fixed with paraformaldehyde and blocked with 10% BSA for 2 hours at room temperature; antigen retrieval was by heat mediation in citrate buffer. Samples were incubated with primary antibody (1/250 in 5% NGS + 10% BSA in PBS) for 16 hours at 4°C. An HRP-conjugated goat anti-mouse polyclonal IgG (1/250) was used as the secondary antibody. -
ab104836 staining Hexokinase II in K562 cells by Immunofluorescence (green) and negative control (purple).
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All lanes : Anti-Hexokinase II antibody [3D3] (ab104836) at 1/500 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : HEK293 cell lysate
Predicted band size: 102 kDa -
ab104836 at 1/200 dilution staining Hexokinase II in human esophagus cancer by Immunohistochemistry, Paraffin-embedded tissue with DAB staining.
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ab104836 at 1/200 dilution staining Hexokinase II in human lung cancer by Immunohistochemistry, Paraffin-embedded tissue with DAB staining.
Protocols
Datasheets and documents
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Datasheet download
References (66)
ab104836 has been referenced in 66 publications.
- Qian Y et al. CCL2 activates AKT signaling to promote glycolysis and chemoresistance in glioma cells. Cell Biol Int 46:819-828 (2022). PubMed: 35178826
- Lu L et al. Exosomal lncRNA TUG1 from cancer-associated fibroblasts promotes liver cancer cell migration, invasion, and glycolysis by regulating the miR-524-5p/SIX1 axis. Cell Mol Biol Lett 27:17 (2022). PubMed: 35193488
- Park Y et al. Impact of Exogenous Treatment with Histidine on Hepatocellular Carcinoma Cells. Cancers (Basel) 14:N/A (2022). PubMed: 35267513
- Wang Y et al. HLA complex P5 upregulation is correlated with poor prognosis and tumor progression in esophageal squamous cell carcinoma. Bioengineered 13:9301-9311 (2022). PubMed: 35389828
- Ishihara S et al. The lactate sensor GPR81 regulates glycolysis and tumor growth of breast cancer. Sci Rep 12:6261 (2022). PubMed: 35428832