Product nameAnti-Hexokinase II antibody
See all Hexokinase II primary antibodies
DescriptionRabbit polyclonal to Hexokinase II
Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
Species reactivityReacts with: Human
This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected within aa 400~500 of human HK2.
- A375 cell lysate; Human hepatocarcinoma tissue
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Concentration information loading...
PurityProtein G purified
Purification notesThis antibody is purified through a protein G column and eluted out with both high and low pH buffers and neutralized immediately after elution then followed by dialysis against PBS.
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Our Abpromise guarantee covers the use of ab37593 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/100 - 1/500. Detects a band of approximately 102 kDa (predicted molecular weight: 102 kDa).|
|IHC-P||1/50 - 1/100.|
Tissue specificityPredominant hexokinase isozyme expressed in insulin-responsive tissues such as skeletal muscle.
PathwayCarbohydrate metabolism; hexose metabolism.
Sequence similaritiesBelongs to the hexokinase family.
Contains 2 hexokinase domains.
DomainThe N- and C-terminal halves of this hexokinase show extensive sequence similarity to each other. The catalytic activity is associated with the C-terminus while regulatory function is associated with the N-terminus. Each domain can bind a single glucose and Gluc-6-P molecule.
Cellular localizationMitochondrion outer membrane. Its hydrophobic N-terminal sequence may be involved in membrane binding.
- Information by UniProt
- DKFZp686M1669 antibody
- Hexokinase 2 antibody
- Hexokinase 2 muscle antibody
Anti-Hexokinase II antibody (ab37593) at 1/500 dilution + A375 cell lysate
Predicted band size: 102 kDa
Observed band size: 102 kDa
Additional bands at: 30 kDa, 40 kDa, 60 kDa, 75 kDa. We are unsure as to the identity of these extra bands.
ab37593 staining Hexokinase II in Human renal cancer tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% serum for 20 minutes; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/200) for 45 minutes at 22°C. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/400) was used as the secondary antibody.
ab37593, at 1/100 dilution, staining human hepatocarcinoma tissue by immunohistochemistry. Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
This product has been referenced in:
- Huang FT et al. The novel long intergenic noncoding RNA UCC promotes colorectal cancer progression by sponging miR-143. Cell Death Dis 8:e2778 (2017). WB ; Human . Read more (PubMed: 28492554) »
- Zhao Y et al. MicroRNA-33b inhibits cell proliferation and glycolysis by targeting hypoxia-inducible factor-1a in malignant melanoma. Exp Ther Med 14:1299-1306 (2017). Read more (PubMed: 28781622) »