Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20839] to Hexokinase II (HRP)
- Suitable for: IHC-P
- Reacts with: Human
- Conjugation: HRP
Product nameAnti-Hexokinase II antibody [EPR20839] (HRP)
See all Hexokinase II primary antibodies
DescriptionRabbit monoclonal [EPR20839] to Hexokinase II (HRP)
Tested applicationsSuitable for: IHC-Pmore details
Species reactivityReacts with: Human
Recombinant fragment within Human Hexokinase II aa 1-500. The exact sequence is proprietary.
Database link: P52789
- IHC-P: Human pancreas adenocarcinoma tissue sections.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol, 1% BSA, PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab237315 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
Tissue specificityPredominant hexokinase isozyme expressed in insulin-responsive tissues such as skeletal muscle.
PathwayCarbohydrate metabolism; hexose metabolism.
Sequence similaritiesBelongs to the hexokinase family.
Contains 2 hexokinase domains.
DomainThe N- and C-terminal halves of this hexokinase show extensive sequence similarity to each other. The catalytic activity is associated with the C-terminus while regulatory function is associated with the N-terminus. Each domain can bind a single glucose and Gluc-6-P molecule.
Cellular localizationMitochondrion outer membrane. Its hydrophobic N-terminal sequence may be involved in membrane binding.
- Information by UniProt
- DKFZp686M1669 antibody
- Hexokinase 2 antibody
- Hexokinase 2 muscle antibody
IHC image of Hexokinase II staining in a section of formalin-fixed paraffin-embedded human pancreas adenocarcinoma*, performed on a Leica BOND™.
The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH9, epitope retrieval solution 2) for 20mins. The section was then incubated with abab237315, 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab237315 has not yet been referenced specifically in any publications.