Overview

  • Product name

    Hexokinase II Inhibitor Assay Kit (Colorimetric)
    See all Hexokinase II kits
  • Detection method

    Colorimetric
  • Assay time

    0h 30m
  • Product overview

    Hexokinase II Inhibitor Screening Kit (Colorimetric) (ab211114) provides a sensitive, quick, and easy method for screening potential inhibitors of Hexokinase II (HK-II) in less than 30 minutes. The assay is based in the ability of HK-II to convert glucose into glucose-6-phosphate. Glucose-6-phosphate is oxidized by glucose-6- phosphate dehydrogenase to form NADH, which reduces a probe that shows strong absorbance at OD 450 nm. In the presence of a HK-II inhibitor such as Bromopyruvic Acid, the reaction is impeded, decreasing the rate or extent of generation of HK-II–dependent absorbance at OD 450 nm.


    This simple and high-throughput adaptable assay kit can be used to screen, study or characterize potential inhibitors of Hexokinase II.

  • Notes

    Hexokinases (HK, EC: 2.7.1.1) are found in many organisms including bacteria, plants and mammals and play an important role in glucose metabolism. The Hexokinase family phosphorylates glucose and generates glucose-6-phosphate for glycolysis. Four Hexokinase isoforms (HK-I, II, III and IV) are found in numerous species. HK-I, HK-II and HK-III have low affinity for glucose, one of their natural substrates, while HK-IV show high affinity for this monosaccharide. Recent studies have found increased hexokinase activity in various human metastatic tumors. Moreover, Hexokinase II (HKII) is the main isoform of the Hexokinases and is responsible for malignant phenotypes. HKII binds to the outer mitochondrial membrane via the Voltage-Dependent Anion Channel (VDAC), a Porin-like protein. HKII has become an attractive therapeutic target for its role in cancer metastasis.

  • Platform

    Microplate reader

Properties

Associated products

Images

  • Typical inhibition curve of human Hexokinase II activity by the hexokinase inhibitor Bromopyruvic Acid. IC50 was determined to be 3 µM. Assay was performed following the kit protocol.

     

Protocols

References

ab211114 has not yet been referenced specifically in any publications.

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