Key features and details
- Assay type: Semi-quantitative
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 30 min
- Sample type: Inhibitor compounds
Product nameHexokinase Inhibitor Assay Kit (Colorimetric)
See all Hexokinase kits
Sample typeInhibitor compounds
Assay time0h 30m
Hexokinase Inhibitor Screening Kit (Colorimetric) (ab211107) provides a rapid, simple and adaptable test for high-throughput screening of Hexokinase inhibitors within 30 minutes. Glucose is converted to glucose-6-phosphate by hexokinase; the glucose-6-phosphate is oxidized by glucose-6-phosphate dehydrogenase to form NADH, which reduces a colorless probe to a colored product with strong absorbance at OD = 450 nm. In the presence of a Hexokinase inhibitor, the reaction is impeded/abolished resulting in decrease or total loss of absorbance.
This simple and high-throughput adaptable assay kit can be used to screen, study or characterize potential inhibitors of Hexokinase.
Hexokinase (HK, 6-Phosphate glucose kinase, ATP:D-Hexose 6- Phosphotransferase, ATP-dependent hexokinase, EC 220.127.116.11) is responsible for phosphorylating hexoses (six-carbon sugars) to form hexose phosphate. Hexokinases play an important role in glucose metabolism, as glucose is the most important substrate of hexokinases. Hexokinases are found in many organisms including bacteria, plants and mammals. In mammals, there are four isoforms (HK-I, II, III and IV). HK-I, HK-II, and HK-III are referred as “low Km” because of their high affinity for glucose (Km < 1mM), while HK-IV (also known as Glucokinase) has a Km for glucose 100-fold higher and can only phosphorylate glucose when the substrate concentration is high enough.
Hexokinase deficiency leads to diseases such as X-linked muscular dystrophy and rare autosomal recessive hemolytic anemia. On the other hand, increased hexokinase activity is detected in various human tumors and is associated with metastasis.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 100 tests HK Assay Buffer 1 x 25ml Hexokinase (0.5 U) 1 vial HK Coenzyme (25 mg) 1 vial HK Converter (10 U) 1 vial HK Developer (30 mg) 1 vial HK Inhibitor Control (40 µmole) 1 vial HK Substrate 1 x 1ml
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
ab211107 has not yet been referenced specifically in any publications.