Overview

  • Product name

    Hexokinase Inhibitor Assay Kit (Colorimetric)
    See all Hexokinase kits
  • Detection method

    Colorimetric
  • Sample type

    Inhibitor compounds
  • Assay type

    Semi-quantitative
  • Assay time

    0h 30m
  • Product overview

    Hexokinase Inhibitor Screening Kit (Colorimetric) (ab211107) provides a rapid, simple and adaptable test for high-throughput screening of Hexokinase inhibitors within 30 minutes. Glucose is converted to glucose-6-phosphate by hexokinase; the glucose-6-phosphate is oxidized by glucose-6-phosphate dehydrogenase to form NADH, which reduces a colorless probe to a colored product with strong absorbance at OD = 450 nm. In the presence of a Hexokinase inhibitor, the reaction is impeded/abolished resulting in decrease or total loss of absorbance.


    This simple and high-throughput adaptable assay kit can be used to screen, study or characterize potential inhibitors of Hexokinase.

  • Notes

    Hexokinase (HK, 6-Phosphate glucose kinase, ATP:D-Hexose 6- Phosphotransferase, ATP-dependent hexokinase, EC 1.1.1.49) is responsible for phosphorylating hexoses (six-carbon sugars) to form hexose phosphate. Hexokinases play an important role in glucose metabolism, as glucose is the most important substrate of hexokinases. Hexokinases are found in many organisms including bacteria, plants and mammals. In mammals, there are four isoforms (HK-I, II, III and IV). HK-I, HK-II, and HK-III are referred as “low Km” because of their high affinity for glucose (Km < 1mM), while HK-IV (also known as Glucokinase) has a Km for glucose 100-fold higher and can only phosphorylate glucose when the substrate concentration is high enough.

    Hexokinase deficiency leads to diseases such as X-linked muscular dystrophy and rare autosomal recessive hemolytic anemia. On the other hand, increased hexokinase activity is detected in various human tumors and is associated with metastasis.

  • Platform

    Microplate reader

Properties

Images

  • Typical inhibition curve of Hexokinase activity by Hexokinase Inhibitor (Bromopyruvic Acid). Assay was performed following the kit protocol.

Protocols

References

ab211107 has not yet been referenced specifically in any publications.

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