Key features and details
- Rabbit polyclonal to Hexokinase Type III/HK3
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Hexokinase Type III/HK3 antibody
See all Hexokinase Type III/HK3 primary antibodies
DescriptionRabbit polyclonal to Hexokinase Type III/HK3
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Recombinant fragment corresponding to Human Hexokinase Type III/HK3 aa 587-786.
Database link: P52790
- HeLa whole cell lysate (ab150035); A549 and HCT116 cell lysates; HeLa cells
This product was previously labelled as Hexokinase Type III
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Our Abpromise guarantee covers the use of ab126217 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 99 kDa.|
|ICC/IF||1/100 - 1/500.|
PathwayCarbohydrate metabolism; hexose metabolism.
Sequence similaritiesBelongs to the hexokinase family.
DomainThe N- and C-terminal halves of this hexokinase show extensive sequence similarity to each other. The catalytic activity is associated with the C-terminus while regulatory function is associated with the N-terminus.
- Information by UniProt
- ATP:D hexose 6 phosphotransferase antibody
- Hexokinase type III antibody
- Hexokinase-3 antibody
Anti-Hexokinase Type III/HK3 antibody (ab126217) at 1/1000 dilution + Hela whole cell lysate at 30 µg
Predicted band size: 99 kDa
7.5% SDS PAGE
ab126217, at 1/500 dilution, staining Hexokinase Type III/HK3 in methanol fixed Hela cells by Immunofluorescence.
The image in the lower panel was costained with Hoechst 33342.
ab126217 has been referenced in 3 publications.
- Mehta MM et al. Hexokinase 2 is dispensable for T cell-dependent immunity. Cancer Metab 6:10 (2018). PubMed: 30140438
- Kuang R et al. GLUT3 upregulation promotes metabolic reprogramming associated with antiangiogenic therapy resistance. JCI Insight 2:e88815 (2017). WB ; Human . PubMed: 28138554
- Cao Y et al. Metabolic reprogramming towards aerobic glycolysis correlates with greater proliferative ability and resistance to metabolic inhibition in CD8 versus CD4 T cells. PLoS One 9:e104104 (2014). WB ; Mouse . PubMed: 25090630