Product nameAnti-Hexokinase Type III/HK3 antibody
See all Hexokinase Type III/HK3 primary antibodies
DescriptionRabbit polyclonal to Hexokinase Type III/HK3
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Recombinant fragment within Human Hexokinase Type III/HK3. The exact sequence is proprietary. Center region.
Database link: P52790
- WB: HEK-293T, A431 and HeLa whole cell lysate. IHC-P: Human hepatoma tissue. ICC/IF: HeLa cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 1% BSA, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab272872 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000.|
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
|ICC/IF||1/100 - 1/1000.|
PathwayCarbohydrate metabolism; hexose metabolism.
Sequence similaritiesBelongs to the hexokinase family.
DomainThe N- and C-terminal halves of this hexokinase show extensive sequence similarity to each other. The catalytic activity is associated with the C-terminus while regulatory function is associated with the N-terminus.
- Information by UniProt
- ATP:D hexose 6 phosphotransferase antibody
- Hexokinase type III antibody
- Hexokinase-3 antibody
All lanes : Anti-Hexokinase Type III/HK3 antibody (ab272872) at 1/3000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lysates/proteins at 30 µg per lane.
5% SDS PAGE
Paraffin-embedded human hepatoma tissue stained for Hexokinase Type III/HK3 using ab272872 at 1/500 dilution in immunohistochemical analysis. Antigen retrieval using EDTA based, pH 8.0 buffer, 15 minutes.
Ice-cold methanol fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Hexokinase Type III/HK3 using ab272872 at 1/500 dilution in ICC/IF (green).
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab272872 has not yet been referenced specifically in any publications.