Key features and details
- Rabbit polyclonal to Hey L
- Suitable for: WB, ICC/IF, ELISA
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Hey L antibody
See all Hey L primary antibodies
DescriptionRabbit polyclonal to Hey L
Tested applicationsSuitable for: WB, ICC/IF, ELISAmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Dog
- Foetal muscle lysate.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab26138 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 35 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|ICC/IF||Use at an assay dependent concentration.|
FunctionDownstream effector of Notch signaling which may be required for cardiovascular development (By similarity). Transcriptional repressor which binds preferentially to the canonical E box sequence 5'-CACGTG-3' (By similarity). Represses transcription by the cardiac transcriptional activators GATA4 and GATA6.
Sequence similaritiesBelongs to the HEY family.
Contains 1 basic helix-loop-helix (bHLH) domain.
Contains 1 Orange domain.
- Information by UniProt
- bHLHb33 antibody
- Class B basic helix loop helix protein 33 antibody
- Class B basic helix-loop-helix protein 33 antibody
Anti-Hey L antibody (ab26138) at 1.25 µg/ml + Foetal muscle lysate
HRP conjugated anti-Rabbit IgG
Predicted band size: 35 kDa
Immunofluorescence analysis of HUVEC cells, staining Hey L with ab26138.
Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 1% BSA for 30 minutes at 25°C. Samples were incubated with primary antibody (1/200 in diluent) for 16 hours at 4°C. An AlexaFluor®488-conjugated goat anti-rabbit polyclonal IgG (1/500) was used as the secondary antibody.
ab26138 has been referenced in 1 publication.
- Morrow D et al. Sonic Hedgehog induces Notch target gene expression in vascular smooth muscle cells via VEGF-A. Arterioscler Thromb Vasc Biol 29:1112-8 (2009). PubMed: 19407245