Overview

  • Product name
  • Description
    Rabbit polyclonal to Hey L
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IF, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog
  • Immunogen

    A region within synthetic peptide: MKRPKEPSGS DGESDGPIDV GQEGQLSQMA RPLSTPSSSQ MQARKKRRGI, corresponding to N terminal amino acids 1-50 of Human Hey L

  • Positive control
    • Foetal muscle lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab26138 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 35 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ICC/IF Use at an assay dependent concentration.
ELISA 1/312500.

Target

  • Function
    Downstream effector of Notch signaling which may be required for cardiovascular development (By similarity). Transcriptional repressor which binds preferentially to the canonical E box sequence 5'-CACGTG-3' (By similarity). Represses transcription by the cardiac transcriptional activators GATA4 and GATA6.
  • Sequence similarities
    Belongs to the HEY family.
    Contains 1 basic helix-loop-helix (bHLH) domain.
    Contains 1 Orange domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • bHLHb33 antibody
    • Class B basic helix loop helix protein 33 antibody
    • Class B basic helix-loop-helix protein 33 antibody
    • Hairy enhancer of split related with YRPW motif like antibody
    • Hairy related transcription factor 3 antibody
    • Hairy-related transcription factor 3 antibody
    • Hairy/enhancer of split related with YRPW motif 3 antibody
    • Hairy/enhancer of split related with YRPW motif like antibody
    • Hairy/enhancer of split related with YRPW motif like protein antibody
    • Hairy/enhancer-of-split related with YRPW motif-like protein antibody
    • HEY like protein antibody
    • HEY3 antibody
    • heyl antibody
    • HEYL protein antibody
    • HEYL_HUMAN antibody
    • hHeyL antibody
    • hHRT3 antibody
    • HRT 3 antibody
    • HRT-3 antibody
    • HRT3 antibody
    • MGC12623 antibody
    see all

Images

  • Anti-Hey L antibody (ab26138) at 1.25 µg/ml + Foetal muscle lysate

    Secondary
    HRP conjugated anti-Rabbit IgG

    Predicted band size: 35 kDa

  • Immunofluorescence analysis of HUVEC cells, staining Hey L with ab26138.

    Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 1% BSA for 30 minutes at 25°C. Samples were incubated with primary antibody (1/200 in diluent) for 16 hours at 4°C. An AlexaFluor®488-conjugated goat anti-rabbit polyclonal IgG (1/500) was used as the secondary antibody.

    See Abreview

References

This product has been referenced in:
  • Morrow D  et al. Sonic Hedgehog induces Notch target gene expression in vascular smooth muscle cells via VEGF-A. Arterioscler Thromb Vasc Biol 29:1112-8 (2009). Read more (PubMed: 19407245) »
See 1 Publication for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Monkey Cell (COS1 cell line)
Specification
COS1 cell line
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton X100
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Feb 11 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HUVEK cells)
Specification
HUVEK cells
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton X100
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Nov 23 2012

Question

DESCRIPTION OF THE PROBLEM No staining.I used the antibody as the immunogen used (Human HeyL 1-50) was very close to the mouse HeyL protein which matched 49 of the aa. However it did not give any staining in the positive control (mouse E11.5 embryo section) with or without antigen retreival. I have seen that recently you have added two chicken anti HeyL antibodies which I would like to try though I am not sure if I should spend another $300 to get an antibody that does not work. SAMPLE Tissue PRIMARY ANTIBODY HeyL ab26138 Abcam DETECTION METHOD Immunofluorescence POSITIVE AND NEGATIVE CONTROLS USED Positive - Mouse E11.5 embryo section (worked in in situ hybridization) Negative - Secondary antibody control ANTIBODY STORAGE CONDITIONS -20 C (aliquots) FIXATION OF SAMPLE Paraformaldehyde ANTIGEN RETRIEVAL Heat mediated (sodium citrate) PERMEABILIZATION STEP 0.25% Triton X BLOCKING CONDITIONS 1% BSA with 0.25% Triton X 100, 10% NGS SECONDARY ANTIBODY Goat anti Rabbit IgG. Invitrogen Molecular Probes HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 2 HAVE YOU RUN A "NO PRIMARY" CONTROL? Yes DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes WHAT STEPS HAVE YOU ALTERED? Antigen retrieval ADDITIONAL NOTES I used the antibody as the immunogen used (Human HeyL 1-50) was very close to the mouse HeyL protein which matched 49 of the aa. However it did not give any staining in the positive control (mouse E11.5 embryo section) with or without antigen retreival. I have seen that recently you have added two chicken anti HeyL antibodies which I would like to try though I am not sure if I should spend another $300 to get an antibody that does not work.

Read More
Answer

Thank you for your enquiry. I am sorry to hear that you have been having difficulties with this antibody. Rabbit polyclonal to Hey L (ab26138) has been tested and shown to work in ELISA and western blotting. To date it has not been tested for its application in mouse. However, as you mention given the sequence similarity in the immunogen I would certainly predict reactivity in this species. Unfortunately we cannot guarantee this antibody to work for this purpose as it is yet to be tested. The antibody may be incapable of recognizing its epitope under these conditions. I would like to recommend that you leave some feedback of your experience with this antibody by navigating to the antibody datasheet and clicking on the "Abreviews" tab. For doing so you will be awarded with "Abpoints" that can be used to redeem discounts against future purchases. For further information please see www.abcam.com/abreviews.

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