• Product name

  • Description

    Rabbit polyclonal to HEY1
  • Host species

  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chimpanzee, Macaque monkey, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human HEY1 aa 150-250 conjugated to keyhole limpet haemocyanin.
    Database link: Q9Y5J3

  • Positive control

    • This antibody gave a positive signal in Human Spinal Cord tissue lysate.



Our Abpromise guarantee covers the use of ab111723 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 33 kDa.


  • Function

    Downstream effector of Notch signaling which may be required for cardiovascular development. Transcriptional repressor which binds preferentially to the canonical E box sequence 5'-CACGTG-3'. Represses transcription by the cardiac transcriptional activators GATA4 and GATA6.
  • Tissue specificity

    Expressed in the somitic mesoderm, the central nervous system, the kidney, the heart, nasal epithelium, and limbs.
  • Sequence similarities

    Belongs to the HEY family.
    Contains 1 basic helix-loop-helix (bHLH) domain.
    Contains 1 Orange domain.
  • Cellular localization

  • Information by UniProt
  • Database links

  • Alternative names

    • Basic helix loop helix protein OAF1 antibody
    • bHLHb31 antibody
    • Cardiovascular helix loop-helix factor 2 antibody
    • Cardiovascular helix-loop-helix factor 2 antibody
    • CHF-2 antibody
    • CHF2 antibody
    • Class B basic helix-loop-helix protein 31 antibody
    • Hairy and enhancer of split related protein 1 antibody
    • Hairy and enhancer of split-related protein 1 antibody
    • Hairy enhancer-of-split related with YRPW motif protein 1 antibody
    • Hairy related transcription factor 1 antibody
    • Hairy-related transcription factor 1 antibody
    • Hairy/enhancer of split related with YRPW motif 1 antibody
    • Hairy/enhancer-of-split related with YRPW motif protein 1 antibody
    • HERP2 antibody
    • HES related repressor protein 1 antibody
    • HES-related repressor protein 1 antibody
    • HES-related repressor protein 2 antibody
    • HESR-1 antibody
    • HESR1 antibody
    • HEY 1 antibody
    • hey1 antibody
    • HEY1_HUMAN antibody
    • hHRT1 antibody
    • HRT-1 antibody
    • HRT1 antibody
    • OAF1 antibody
    see all


  • Anti-HEY1 antibody (ab111723) at 1 µg/ml + Spinal Cord (Human) Tissue Lysate - adult normal tissue at 25 µg

    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 33 kDa
    Observed band size: 34 kDa
    why is the actual band size different from the predicted?

    Exposure time: 16 minutes

    This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab111723 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.


ab111723 has not yet been referenced specifically in any publications.

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