Product nameAnti-HEY1 antibody
See all HEY1 primary antibodies
DescriptionRabbit polyclonal to HEY1
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Chimpanzee, Macaque monkey, Orangutan
Synthetic peptide corresponding to Human HEY1 aa 150-250 conjugated to keyhole limpet haemocyanin.
Database link: Q9Y5J3
- This antibody gave a positive signal in Human Spinal Cord tissue lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab111723 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 33 kDa.|
FunctionDownstream effector of Notch signaling which may be required for cardiovascular development. Transcriptional repressor which binds preferentially to the canonical E box sequence 5'-CACGTG-3'. Represses transcription by the cardiac transcriptional activators GATA4 and GATA6.
Tissue specificityExpressed in the somitic mesoderm, the central nervous system, the kidney, the heart, nasal epithelium, and limbs.
Sequence similaritiesBelongs to the HEY family.
Contains 1 basic helix-loop-helix (bHLH) domain.
Contains 1 Orange domain.
- Information by UniProt
- Basic helix loop helix protein OAF1 antibody
- bHLHb31 antibody
- Cardiovascular helix loop-helix factor 2 antibody
Anti-HEY1 antibody (ab111723) at 1 µg/ml + Spinal Cord (Human) Tissue Lysate - adult normal tissue at 25 µg
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 33 kDa
Observed band size: 34 kDa why is the actual band size different from the predicted?
Exposure time: 16 minutes
This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab111723 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
ab111723 has not yet been referenced specifically in any publications.