Anti-HEY1 antibody (ab235173)
Key features and details
- Rabbit polyclonal to HEY1
- Suitable for: WB, IHC
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-HEY1 antibody
See all HEY1 primary antibodies -
Description
Rabbit polyclonal to HEY1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHCmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Cow, Dog -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human cerebellum tissue. Hey1 variant 2 human recombinant protein. WB: A172 cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab235173 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Predicted molecular weight: 33 kDa.
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IHC |
1/100 - 1/200.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Notes |
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WB
1/1000. Predicted molecular weight: 33 kDa. |
IHC
1/100 - 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
Downstream effector of Notch signaling which may be required for cardiovascular development. Transcriptional repressor which binds preferentially to the canonical E box sequence 5'-CACGTG-3'. Represses transcription by the cardiac transcriptional activators GATA4 and GATA6. -
Tissue specificity
Expressed in the somitic mesoderm, the central nervous system, the kidney, the heart, nasal epithelium, and limbs. -
Sequence similarities
Belongs to the HEY family.
Contains 1 basic helix-loop-helix (bHLH) domain.
Contains 1 Orange domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 408005 Cow
- Entrez Gene: 403420 Dog
- Entrez Gene: 23462 Human
- Entrez Gene: 15213 Mouse
- Omim: 602953 Human
- SwissProt: Q2KIN4 Cow
- SwissProt: Q9TSZ2 Dog
- SwissProt: Q9Y5J3 Human
see all -
Alternative names
- Basic helix loop helix protein OAF1 antibody
- bHLHb31 antibody
- Cardiovascular helix loop-helix factor 2 antibody
see all
Images
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Immunohistochemical analysis of human cerebellum tissue labeling Hey1 with ab235173 at 1/200 dilution (30 minutes at RT), followed by a ready to use anti-Rabbit polyclonal IgG (HRP) (8 minutes at RT). Primary staining is DAB. Counterstained with hematoxylin.
Perform heat mediated antigen retrieval using citrate buffer for 20 minutes.
Panel A: H&E staining.
Panel B: ab235173 at 20X magnification.
Panel C: ab235173 at 40X magnification. -
All lanes : Anti-HEY1 antibody (ab235173) at 1/1000 dilution
Lane 1 : Hey1 variant 2 human recombinant protein, 50ng
Lane 2 : A-172 lysate at 35 µg
Lane 3 : Jurkat cell lysate at 35 µg
Lane 4 : Mouse brain tissue lysate at 35 µg
Secondary
All lanes : Goat anti Rabbit IgG [H&L] HRP at 1/70000 dilution
Predicted band size: 33 kDa
Exposure time: 19 secondsImaged on Chemidoc program.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (1)
ab235173 has been referenced in 1 publication.
- Woods AD et al. Metastatic pediatric sclerosing epithelioid fibrosarcoma. Cold Spring Harb Mol Case Stud 7:N/A (2021). PubMed: 34362827