Key features and details
- Rabbit polyclonal to HEY1
- Suitable for: WB, IHC
- Reacts with: Human
- Isotype: IgG
Product nameAnti-HEY1 antibody
See all HEY1 primary antibodies
DescriptionRabbit polyclonal to HEY1
Tested applicationsSuitable for: WB, IHCmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Cow, Dog
Synthetic peptide. This information is considered to be commercially sensitive.
- IHC-P: Human cerebellum tissue. Hey1 variant 2 human recombinant protein. WB: A172 cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Concentration information loading...
Our Abpromise guarantee covers the use of ab235173 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 33 kDa.|
|IHC||1/100 - 1/200.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
FunctionDownstream effector of Notch signaling which may be required for cardiovascular development. Transcriptional repressor which binds preferentially to the canonical E box sequence 5'-CACGTG-3'. Represses transcription by the cardiac transcriptional activators GATA4 and GATA6.
Tissue specificityExpressed in the somitic mesoderm, the central nervous system, the kidney, the heart, nasal epithelium, and limbs.
Sequence similaritiesBelongs to the HEY family.
Contains 1 basic helix-loop-helix (bHLH) domain.
Contains 1 Orange domain.
- Information by UniProt
- Basic helix loop helix protein OAF1 antibody
- bHLHb31 antibody
- Cardiovascular helix loop-helix factor 2 antibody
Immunohistochemical analysis of human cerebellum tissue labeling Hey1 with ab235173 at 1/200 dilution (30 minutes at RT), followed by a ready to use anti-Rabbit polyclonal IgG (HRP) (8 minutes at RT). Primary staining is DAB. Counterstained with hematoxylin.
Perform heat mediated antigen retrieval using citrate buffer for 20 minutes.
Panel A: H&E staining.
Panel B: ab235173 at 20X magnification.
Panel C: ab235173 at 40X magnification.
All lanes : Anti-HEY1 antibody (ab235173) at 1/1000 dilution
Lane 1 : Hey1 variant 2 human recombinant protein, 50ng
Lane 2 : A-172 lysate at 35 µg
Lane 3 : Jurkat cell lysate at 35 µg
Lane 4 : Mouse brain tissue lysate at 35 µg
All lanes : Goat anti Rabbit IgG [H&L] HRP at 1/70000 dilution
Predicted band size: 33 kDa
Exposure time: 19 seconds
Imaged on Chemidoc program.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab235173 has not yet been referenced specifically in any publications.