Overview

  • Product name

  • Description

    Rabbit polyclonal to HEY2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    3 synthetic peptides (Human) conjugated to KLH

Properties

Applications

Our Abpromise guarantee covers the use of ab86010 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).

Target

  • Function

    Downstream effector of Notch signaling which may be required for cardiovascular development. Transcriptional repressor which binds preferentially to the canonical E box sequence 5'-CACGTG-3'. Represses transcription by the cardiac transcriptional activators GATA4 and GATA6.
  • Sequence similarities

    Belongs to the HEY family.
    Contains 1 basic helix-loop-helix (bHLH) domain.
    Contains 1 Orange domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • bHLHb32 antibody
    • Cardiovascular helix loop helix factor 1 antibody
    • Cardiovascular helix-loop-helix factor 1 antibody
    • CHF1 antibody
    • Class B basic helix-loop-helix protein 32 antibody
    • GRIDLOCK antibody
    • GRL antibody
    • Hairy and enhancer of split related 2 antibody
    • Hairy and enhancer of split-related protein 2 antibody
    • Hairy related transcription factor 2 antibody
    • Hairy-related transcription factor 2 antibody
    • Hairy/enhancer-of-split related with YRPW motif protein 2 antibody
    • hCHF1 antibody
    • HERP antibody
    • HERP1 antibody
    • HES related repressor protein 1 antibody
    • HES-related repressor protein 2 antibody
    • HESR-2 antibody
    • HESR2 antibody
    • hey2 antibody
    • HEY2_HUMAN antibody
    • hHRT2 antibody
    • HRT-2 antibody
    • HRT2 antibody
    • MGC10720 antibody
    • Protein gridlock homolog antibody
    see all

Images

  • All lanes : Anti-HEY2 antibody (ab86010) at 1/1000 dilution

    Lane 1 : Uninduced culture
    Lane 2 : Induced culture of E.coli

    Secondary
    All lanes : anti-Rabbit IgG HRP at 1/1000 dilution

    Predicted band size: 50 kDa

References

This product has been referenced in:

  • Xu H  et al. NFIX Circular RNA Promotes Glioma Progression by Regulating miR-34a-5p via Notch Signaling Pathway. Front Mol Neurosci 11:225 (2018). Read more (PubMed: 30072869) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
IHC - Wholemount
Sample
Mouse Tissue (Cochlea)
Specification
Cochlea

Ms. Michelle Randle

Verified customer

Submitted Mar 24 2015

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HEY2 overexpressing human embryonic stem cells)
Permeabilization
Yes - saponin
Specification
HEY2 overexpressing human embryonic stem cells
Blocking step
Serum as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Jan 12 2015

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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