Overview

  • Product name
    Anti-HEY2 antibody
    See all HEY2 primary antibodies
  • Description
    Rabbit polyclonal to HEY2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    3 synthetic peptides (Human) conjugated to KLH

Properties

Applications

Our Abpromise guarantee covers the use of ab86010 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).

Target

  • Function
    Downstream effector of Notch signaling which may be required for cardiovascular development. Transcriptional repressor which binds preferentially to the canonical E box sequence 5'-CACGTG-3'. Represses transcription by the cardiac transcriptional activators GATA4 and GATA6.
  • Sequence similarities
    Belongs to the HEY family.
    Contains 1 basic helix-loop-helix (bHLH) domain.
    Contains 1 Orange domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
    • Alternative names
      • bHLHb32 antibody
      • Cardiovascular helix loop helix factor 1 antibody
      • Cardiovascular helix-loop-helix factor 1 antibody
      • CHF1 antibody
      • Class B basic helix-loop-helix protein 32 antibody
      • GRIDLOCK antibody
      • GRL antibody
      • Hairy and enhancer of split related 2 antibody
      • Hairy and enhancer of split-related protein 2 antibody
      • Hairy related transcription factor 2 antibody
      • Hairy-related transcription factor 2 antibody
      • Hairy/enhancer-of-split related with YRPW motif protein 2 antibody
      • hCHF1 antibody
      • HERP antibody
      • HERP1 antibody
      • HES related repressor protein 1 antibody
      • HES-related repressor protein 2 antibody
      • HESR-2 antibody
      • HESR2 antibody
      • hey2 antibody
      • HEY2_HUMAN antibody
      • hHRT2 antibody
      • HRT-2 antibody
      • HRT2 antibody
      • MGC10720 antibody
      • Protein gridlock homolog antibody
      see all

    Images

    • Western blot - Anti-HEY2 antibody (ab86010)
      Western blot - Anti-HEY2 antibody (ab86010)
      All lanes : Anti-HEY2 antibody (ab86010) at 1/1000 dilution

      Lane 1 : Uninduced culture
      Lane 2 : Induced culture of E.coli

      Secondary
      All lanes : anti-Rabbit IgG HRP at 1/1000 dilution

      Predicted band size: 50 kDa

    References

    ab86010 has not yet been referenced specifically in any publications.

    Publishing research using ab86010? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    2 Abreviews

    10
    Votes: Highest First

    IHC - Wholemount abreview for Anti-HEY2 antibody

     Inconclusive
    Abreviews
    Application
    IHC - Wholemount
    Sample
    Mouse Tissue (Cochlea)
    Specification
    Cochlea
    Read More
    abreview image
    Username

    Ms. Michelle Randle

    Verified customer

    Submitted Mar 24 2015

    Immunocytochemistry/ Immunofluorescence abreview for Anti-HEY2 antibody

     Average
    Abreviews
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HEY2 overexpressing human embryonic stem cells)
    Permeabilization
    Yes - saponin
    Specification
    HEY2 overexpressing human embryonic stem cells
    Blocking step
    Serum as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More
    abreview image
    Username

    Abcam user community

    Verified customer

    Submitted Jan 12 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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