Overview

  • Product name

    Anti-HEY2 antibody - N-terminal
    See all HEY2 primary antibodies
  • Description

    Rabbit polyclonal to HEY2 - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human HEY2 aa 1-136 (N terminal). The exact sequence is proprietary.
    Database link: Q9UBP5

  • Positive control

    • A549, H1299, HCT116 and MCF-7 whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab184642 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 35 kDa.

Target

  • Function

    Downstream effector of Notch signaling which may be required for cardiovascular development. Transcriptional repressor which binds preferentially to the canonical E box sequence 5'-CACGTG-3'. Represses transcription by the cardiac transcriptional activators GATA4 and GATA6.
  • Sequence similarities

    Belongs to the HEY family.
    Contains 1 basic helix-loop-helix (bHLH) domain.
    Contains 1 Orange domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • bHLHb32 antibody
    • Cardiovascular helix loop helix factor 1 antibody
    • Cardiovascular helix-loop-helix factor 1 antibody
    • CHF1 antibody
    • Class B basic helix-loop-helix protein 32 antibody
    • GRIDLOCK antibody
    • GRL antibody
    • Hairy and enhancer of split related 2 antibody
    • Hairy and enhancer of split-related protein 2 antibody
    • Hairy related transcription factor 2 antibody
    • Hairy-related transcription factor 2 antibody
    • Hairy/enhancer-of-split related with YRPW motif protein 2 antibody
    • hCHF1 antibody
    • HERP antibody
    • HERP1 antibody
    • HES related repressor protein 1 antibody
    • HES-related repressor protein 2 antibody
    • HESR-2 antibody
    • HESR2 antibody
    • hey2 antibody
    • HEY2_HUMAN antibody
    • hHRT2 antibody
    • HRT-2 antibody
    • HRT2 antibody
    • MGC10720 antibody
    • Protein gridlock homolog antibody
    see all

Images

  • All lanes : Anti-HEY2 antibody - N-terminal (ab184642) at 1/1000 dilution

    Lane 1 : A549 whole cell lysate/extract
    Lane 2 : H1299 whole cell lysate/extract
    Lane 3 : HCT116 whole cell lysate/extract
    Lane 4 : MCF7 whole cell lysate/extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 35 kDa



    10 % SDS-PAGE

References

ab184642 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HEY2 overexpressing human embryonic stem cells)
Permeabilization
Yes - saponin
Specification
HEY2 overexpressing human embryonic stem cells
Blocking step
Serum as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Apr 21 2015

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