Recombinant
RabMAb

Recombinant Anti-HFE antibody [EPR6751(2)] (ab133639)

Overview

  • Product name

    Anti-HFE antibody [EPR6751(2)]
    See all HFE primary antibodies
  • Description

    Rabbit monoclonal [EPR6751(2)] to HFE
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WBmore details
    Unsuitable for: Flow Cyt,ICC or IHC-P
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues in the intracellular domain of Human HFE (UniProt Q30201).

  • Positive control

    • HeLa, A375 and Caco-2 cell lysates
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab133639 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/10 - 1/100.
WB 1/1000 - 1/10000. Predicted molecular weight: 40 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IHC-P.
  • Target

    • Function

      Binds to transferrin receptor (TFR) and reduces its affinity for iron-loaded transferrin.
    • Tissue specificity

      Expressed in all tissues tested except brain.
    • Involvement in disease

      Defects in HFE are a cause of hemochromatosis (HFE) [MIM:235200]. A disorder of iron metabolism characterized by iron overload. Excess iron is deposited in a variety of organs leading to their failure, and resulting in serious illnesses including cirrhosis, hepatomas, diabetes, cardiomyopathy, arthritis, and hypogonadotropic hypogonadism. Severe effects of the disease usually do not appear until after decades of progressive iron loading.
      Defects in HFE are associated with variegate porphyria (VP) [MIM:176200]. Porphyrias are inherited defects in the biosynthesis of heme, resulting in the accumulation and increased excretion of porphyrins or porphyrin precursors. They are classified as erythropoietic or hepatic, depending on whether the enzyme deficiency occurs in red blood cells or in the liver. VP is the most common form of porphyria in South Africa. It is characterized by skin hyperpigmentation and hypertrichosis, abdominal pain, tachycardia, hypertension and neuromuscular disturbances. High fecal levels of protoporphyrin and coproporphyrin, increased urine uroporphyrins and iron overload are typical markers of the disease. Note=Iron overload due to HFE mutations is a precipitating or exacerbating factor in variegate porphyria.
      Defects in HFE are associated with susceptibility to microvascular complications of diabetes type 7 (MVCD7) [MIM:612635]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis.
    • Sequence similarities

      Belongs to the MHC class I family.
      Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
    • Cellular localization

      Membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • dJ221C16.10.1 antibody
      • Hemochromatosis antibody
      • Hemochromatosis protein antibody
      • Hereditary hemochromatosis protein antibody
      • Hereditary hemochromatosis protein HLA H antibody
      • HFE 1 antibody
      • HFE antibody
      • HFE_HUMAN antibody
      • HFE1 antibody
      • HH antibody
      • High Fe antibody
      • HLA H antibody
      • HLA-H antibody
      • HLAH antibody
      • MGC:150812 antibody
      • MGC10379 antibody
      • MGC103790 antibody
      • MHC class I like protein HFE antibody
      • MVCD7 antibody
      • TFQTL2 antibody
      see all

    Images

    • Lanes 1-2 : Anti-HFE antibody [EPR6751(2)] (ab133639) at 1/1000 dilution
      Lanes 3-4 : Rabbit monoclonal anti-myc tag

      Lanes 1 & 3 : HepG2 whole cell lysate - untransfected
      Lanes 2 & 4 : HepG2 whole cell lysate - transfected with pCMV6 human HFE Tag Flag Myc

      Lysates/proteins at 25 µg per lane.

      Secondary
      All lanes : HRP-conjugated anti-rabbit IgG polyclonal at 1/10000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 40 kDa
      Observed band size: 40 kDa
      Additional bands at: 60 kDa (possible non-specific binding)


      Exposure time: 5 seconds

      See Abreview

    • All lanes : Anti-HFE antibody [EPR6751(2)] (ab133639) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : A375 cell lysate
      Lane 3 : Caco-2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 40 kDa

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    ab133639 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Application
    Western blot
    Loading amount
    25 µg
    Gel Running Conditions
    Reduced Denaturing (12% Homemade SDS-PAGE)
    Sample
    Human Cell lysate - whole cell (HepG2)
    Specification
    HepG2
    Treatment
    Transfected with pCMV6 Human HFE Tag Flag Myc
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

    Miss. Chloe Latour

    Verified customer

    Submitted Sep 26 2014

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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