Product nameAnti-HFE antibody [EPR6751(2)]
See all HFE primary antibodies
DescriptionRabbit monoclonal [EPR6751(2)] to HFE
Tested applicationsSuitable for: IP, WBmore details
Unsuitable for: Flow Cyt,ICC or IHC-P
Species reactivityReacts with: Human
Synthetic peptide corresponding to residues in the intracellular domain of Human HFE (UniProt Q30201).
- HeLa, A375 and Caco-2 cell lysates
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 2.30 x 10 -11 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab133639 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||1/10 - 1/100.|
|WB||1/1000 - 1/10000. Predicted molecular weight: 40 kDa.|
FunctionBinds to transferrin receptor (TFR) and reduces its affinity for iron-loaded transferrin.
Tissue specificityExpressed in all tissues tested except brain.
Involvement in diseaseDefects in HFE are a cause of hemochromatosis (HFE) [MIM:235200]. A disorder of iron metabolism characterized by iron overload. Excess iron is deposited in a variety of organs leading to their failure, and resulting in serious illnesses including cirrhosis, hepatomas, diabetes, cardiomyopathy, arthritis, and hypogonadotropic hypogonadism. Severe effects of the disease usually do not appear until after decades of progressive iron loading.
Defects in HFE are associated with variegate porphyria (VP) [MIM:176200]. Porphyrias are inherited defects in the biosynthesis of heme, resulting in the accumulation and increased excretion of porphyrins or porphyrin precursors. They are classified as erythropoietic or hepatic, depending on whether the enzyme deficiency occurs in red blood cells or in the liver. VP is the most common form of porphyria in South Africa. It is characterized by skin hyperpigmentation and hypertrichosis, abdominal pain, tachycardia, hypertension and neuromuscular disturbances. High fecal levels of protoporphyrin and coproporphyrin, increased urine uroporphyrins and iron overload are typical markers of the disease. Note=Iron overload due to HFE mutations is a precipitating or exacerbating factor in variegate porphyria.
Defects in HFE are associated with susceptibility to microvascular complications of diabetes type 7 (MVCD7) [MIM:612635]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis.
Sequence similaritiesBelongs to the MHC class I family.
Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
- Information by UniProt
- dJ221C16.10.1 antibody
- Hemochromatosis antibody
- Hemochromatosis protein antibody
Lanes 1-2 : Anti-HFE antibody [EPR6751(2)] (ab133639) at 1/1000 dilution
Lanes 3-4 : Rabbit monoclonal anti-myc tag
Lanes 1 & 3 : HepG2 whole cell lysate - untransfected
Lanes 2 & 4 : HepG2 whole cell lysate - transfected with pCMV6 human HFE Tag Flag Myc
Lysates/proteins at 25 µg per lane.
All lanes : HRP-conjugated anti-rabbit IgG polyclonal at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 40 kDa
Observed band size: 40 kDa
Additional bands at: 60 kDa (possible non-specific binding)
Exposure time: 5 seconds
All lanes : Anti-HFE antibody [EPR6751(2)] (ab133639) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : A375 cell lysate
Lane 3 : Caco-2 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 40 kDa
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
ab133639 has not yet been referenced specifically in any publications.