Key features and details
- Rabbit polyclonal to HGD
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-HGD antibody
See all HGD primary antibodies
DescriptionRabbit polyclonal to HGD
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Orangutan
Recombinant fragment corresponding to Human HGD aa 226-445.
DFLIPIAWYEDRQVPGGYTVINKYQGKLFAAKQDVSPFNVVAWHGNYTPY KYNLKNFMVINSVAFDHADPSIFTVLTAKSVRPGVAIADFVIFPPRWGVA DKTFRPPYYHRNCMSEFMGLIRGHYEAKQGGFLPGGGSLHSTMTPHGPDA DCFEKASKVKLAPERIADGTMAFMFESSLSLAVTKWGLKASRCLDENYHK CWEPLKSHFTPNSRNPAEPN
Database link: Q93099
- WB: HeLa and HEK-293T whole cell lysate. IHC-P: Human liver and kidney tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab225926 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
|WB||1/1000 - 1/5000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).|
Tissue specificityHighest expression in the prostate, small intestine, colon, kidney and liver.
PathwayAmino-acid degradation; L-phenylalanine degradation; acetoacetate and fumarate from L-phenylalanine: step 4/6.
Involvement in diseaseDefects in HGD are the cause of alkaptonuria (AKU) [MIM:203500]. AKU is an autosomal recessive error of metabolism characterized by an increase in the level of homogentisic acid. The clinical manifestations of AKU are urine that turns dark on standing and alkalinization, black ochronotic pigmentation of cartilage and collagenous tissues, and spine arthritis.
Sequence similaritiesBelongs to the homogentisate dioxygenase family.
- Information by UniProt
- 2-dioxygenase antibody
- AKU antibody
- FLJ94126 antibody
All lanes : Anti-HGD antibody (ab225926) at 1/1000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
All lanes : Goat polyclonal to Rabbit IgG at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 50 kDa
Observed band size: 50 kDa
Paraffin embedded human liver tissue stained for HGD with ab225926 (1/100 dilution) in immunohistochemical analysis.
Paraffin embedded human kidney tissue stained for HGD with ab225926 (1/100 dilution) in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab225926 has not yet been referenced specifically in any publications.