Key features and details
- Rabbit polyclonal to HGD
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat
- Isotype: IgG
Product nameAnti-HGD antibody
See all HGD primary antibodies
DescriptionRabbit polyclonal to HGD
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat
Predicted to work with: Human, Orangutan
Recombinant fragment (His-T7-tag) corresponding to Mouse HGD aa 190-429. (Expressed in E.coli)
VDVFEETRGYILEVYGVHFELPDLGPIGANGLANPRDFLIPVAWYEDRRV PGGYTVINKFQGKLFACKQDVSPFNVVAWHGNYTPYKYNLENFMVINAVA FDHADPSIFTVLTAKSLRPGVAIADFVIFPPRWGVADKTFRPPYYHRNCM SEFMGLIKGHYEAKQGGFLPGGGSLHSAMTPHGPDADCFEKASKAKLEPE RIADGTMAFMFESSLSLAVTKWGLKTCSCLDENYYKCWEP
Database link: O09173
- WB: Recombinant mouse HGD protein; Mouse and rat liver lysates. IHC-P: Mouse kidney and liver tissues.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab231968 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab231968 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 50 kDa.|
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
Tissue specificityHighest expression in the prostate, small intestine, colon, kidney and liver.
PathwayAmino-acid degradation; L-phenylalanine degradation; acetoacetate and fumarate from L-phenylalanine: step 4/6.
Involvement in diseaseDefects in HGD are the cause of alkaptonuria (AKU) [MIM:203500]. AKU is an autosomal recessive error of metabolism characterized by an increase in the level of homogentisic acid. The clinical manifestations of AKU are urine that turns dark on standing and alkalinization, black ochronotic pigmentation of cartilage and collagenous tissues, and spine arthritis.
Sequence similaritiesBelongs to the homogentisate dioxygenase family.
- Information by UniProt
- 2-dioxygenase antibody
- AKU antibody
- FLJ94126 antibody
Paraffin-embedded mouse liver tissue stained for HGD using ab231968 at 20 µg/ml in immunohistochemical analysis. DAB staining.
Paraffin-embedded mouse kidney tissue stained for HGD using ab231968 at 20 µg/ml in immunohistochemical analysis. DAB staining.
All lanes : Anti-HGD antibody (ab231968) at 3 µg/ml
Lane 1 : Mouse liver lysate
Lane 2 : Rat liver lysate
All lanes : HRP-Linked Guinea pig anti-rabbit at 1/2000 dilution
Predicted band size: 50 kDa
Anti-HGD antibody (ab231968) at 3 µg/ml + Recombinant mouse HGD protein
HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 50 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab231968 has not yet been referenced specifically in any publications.