Overview

  • Product name

    Anti-HGD antibody [EPR7874]
    See all HGD primary antibodies
  • Description

    Rabbit monoclonal [EPR7874] to HGD
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human HGD aa 400-500 (C terminal). The exact sequence is proprietary.

  • Positive control

    • Caco-2, HepG2, and Human fetal liver lysates; Human kidney tissue; HepG2 cells
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab131035 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Tissue specificity

      Highest expression in the prostate, small intestine, colon, kidney and liver.
    • Pathway

      Amino-acid degradation; L-phenylalanine degradation; acetoacetate and fumarate from L-phenylalanine: step 4/6.
    • Involvement in disease

      Defects in HGD are the cause of alkaptonuria (AKU) [MIM:203500]. AKU is an autosomal recessive error of metabolism characterized by an increase in the level of homogentisic acid. The clinical manifestations of AKU are urine that turns dark on standing and alkalinization, black ochronotic pigmentation of cartilage and collagenous tissues, and spine arthritis.
    • Sequence similarities

      Belongs to the homogentisate dioxygenase family.
    • Information by UniProt
    • Database links

    • Alternative names

      • 2-dioxygenase antibody
      • AKU antibody
      • FLJ94126 antibody
      • hgd antibody
      • HGD_HUMAN antibody
      • HGO antibody
      • Homogentisate 1 2 dioxygenase antibody
      • Homogentisate 1 antibody
      • Homogentisate oxidase antibody
      • Homogentisate oxygenase antibody
      • Homogentisic acid oxidase antibody
      • Homogentisicase antibody
      see all

    Images

    • All lanes : Anti-HGD antibody [EPR7874] (ab131035) at 1/1000 dilution

      Lane 1 : Caco-2 cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : Human fetal liver lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 50 kDa
      Observed band size: 50 kDa

    • Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling HGD with ab131035 antibody at 1/100 dilution.
    • Immunofluorescence analysis of HepG2 cells labelling HGD with ab131035 antibody at 1/100 dilution.

    References

    ab131035 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab131035.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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