Recombinant Anti-HGF antibody [EPR12230] (ab178395)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12230] to HGF
- Suitable for: WB, IP
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-HGF antibody [EPR12230]
See all HGF primary antibodies -
Description
Rabbit monoclonal [EPR12230] to HGF -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Synthetic peptide within Human HGF aa 100-200 (Cysteine residue). The exact sequence is proprietary.
Database link: P14210 -
Positive control
- Human placenta and U-87 MG cell lysates and Human HGF (aa 32-728) recombinant protein. IP: Human placenta lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR12230 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab178395 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/200. Predicted molecular weight: 83 kDa.
For unpurified use at 1/1000- 1/5000. |
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IP |
1/10 - 1/100.
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Notes |
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WB
1/200. Predicted molecular weight: 83 kDa. For unpurified use at 1/1000- 1/5000. |
IP
1/10 - 1/100. |
Target
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Function
Potent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types. Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization. -
Involvement in disease
Deafness, autosomal recessive, 39 -
Sequence similarities
Belongs to the peptidase S1 family. Plasminogen subfamily.
Contains 4 kringle domains.
Contains 1 PAN domain.
Contains 1 peptidase S1 domain. - Information by UniProt
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Database links
- Entrez Gene: 3082 Human
- Omim: 142409 Human
- SwissProt: P14210 Human
- Unigene: 396530 Human
- Unigene: 561679 Human
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Alternative names
- DFNB39 antibody
- F TCF antibody
- Fibroblast derived tumor cytotoxic factor antibody
see all
Images
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Anti-HGF antibody [EPR12230] (ab178395) at 1/1000 dilution (Purified) + U-87 MG (Human glioblastoma-astrocytoma epithelial cell) whole cell lysates at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 83 kDa
Observed band size: 83 kDa -
Anti-HGF antibody [EPR12230] (ab178395) at 1/1000 dilution (Purified) + Human HGF (aa 32-728) recombinant protein
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 83 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted? -
Anti-HGF antibody [EPR12230] (ab178395) at 1/200 dilution (Purified) + Human placenta lysates at 15 µg
Secondary
Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 83 kDa
Observed band size: 83 kDa -
ab178395 (purified) at 1:30 dilution (2µg) immunoprecipitating HGF in Human placenta lysate.
Lane 1 (input): Human placenta lysate 10µg
Lane 2 (+): ab178395 & Human placenta lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab178395 in Human placenta lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (10)
ab178395 has been referenced in 10 publications.
- Zhu C et al. Tumor-derived extracellular vesicles inhibit HGF/c-Met and EGF/EGFR pathways to accelerate the radiosensitivity of nasopharyngeal carcinoma cells via microRNA-142-5p delivery. Cell Death Discov 8:17 (2022). PubMed: 35013115
- Sun H et al. TOPK/PBK is phosphorylated by ERK2 at serine 32, promotes tumorigenesis and is involved in sorafenib resistance in RCC. Cell Death Dis 13:450 (2022). PubMed: 35546143
- Chen H et al. Metformin reduces HGF-induced resistance to alectinib via the inhibition of Gab1. Cell Death Dis 11:111 (2020). PubMed: 32041944
- Kurmyshkina O et al. Markers of Angiogenesis, Lymphangiogenesis, and Epithelial-Mesenchymal Transition (Plasticity) in CIN and Early Invasive Carcinoma of the Cervix: Exploring Putative Molecular Mechanisms Involved in Early Tumor Invasion. Int J Mol Sci 21:N/A (2020). PubMed: 32899940
- Hu C et al. Gene transfer of a naked plasmid (pUDK-HGF) encoding human hepatocyte growth factor attenuates skin/muscle incision and retraction-induced chronic post-surgical pain in rats. Eur J Pain 22:961-972 (2018). PubMed: 29377496
- Hu C et al. Gene therapy for neuropathic pain induced by spared nerve injury with naked plasmid encoding hepatocyte growth factor. J Gene Med 19:N/A (2017). PubMed: 29059509
- Deying W et al. CAF-derived HGF promotes cell proliferation and drug resistance by up-regulating the c-Met/PI3K/Akt and GRP78 signalling in ovarian cancer cells. Biosci Rep 37:N/A (2017). PubMed: 28258248
- Kwon MJ et al. Programmed death ligand-1 and MET co-expression is a poor prognostic factor in gastric cancers after resection. Oncotarget 8:82399-82414 (2017). PubMed: 29137273
- Zhang S et al. Synergistic Inhibitory Effect of Traditional Chinese Medicine Astragaloside IV and Curcumin on Tumor Growth and Angiogenesis in an Orthotopic Nude-Mouse Model of Human Hepatocellular Carcinoma. Anticancer Res 37:465-473 (2017). PubMed: 28179291
- Kwon MJ et al. Frequent hepatocyte growth factor overexpression and low frequency of c-Met gene amplification in human papillomavirus-negative tonsillar squamous cell carcinoma and their prognostic significances. Hum Pathol 45:1327-38 (2014). PubMed: 24810547