Recombinant Anti-HGF antibody [EPR12230] - BSA and Azide free (ab240204)
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- References
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Overview
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Product name
Anti-HGF antibody [EPR12230] - BSA and Azide free
See all HGF primary antibodies -
Description
Rabbit monoclonal [EPR12230] to HGF - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Synthetic peptide within Human HGF aa 100-200 (Cysteine residue). The exact sequence is proprietary.
Database link: P14210 -
Positive control
- IP: Human placenta lysate.
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General notes
Ab240204 is the carrier-free version of ab178395. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab240204 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR12230 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab240204 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use at an assay dependent concentration. Predicted molecular weight: 83 kDa. | |
| IP | Use at an assay dependent concentration. |
Target
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Function
Potent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types. Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization. -
Involvement in disease
Deafness, autosomal recessive, 39 -
Sequence similarities
Belongs to the peptidase S1 family. Plasminogen subfamily.
Contains 4 kringle domains.
Contains 1 PAN domain.
Contains 1 peptidase S1 domain. - Information by UniProt
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Database links
- Entrez Gene: 3082 Human
- Omim: 142409 Human
- SwissProt: P14210 Human
- Unigene: 396530 Human
- Unigene: 561679 Human
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Alternative names
- DFNB39 antibody
- F TCF antibody
- Fibroblast derived tumor cytotoxic factor antibody
see all
Images
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![Immunoprecipitation - Anti-HGF antibody [EPR12230] - BSA and Azide free (ab240204)](https://www.abcam.com/ps/products/240/ab240204/Images/ab240204-337462-anti-hgf-antibody-epr12230-immunoprecipitation.jpg)
Immunoprecipitation - Anti-HGF antibody [EPR12230] - BSA and Azide free (ab240204)ab178395 (purified) at 1:30 dilution (2µg) immunoprecipitating HGF in Human placenta lysate.
Lane 1 (input): Human placenta lysate 10µg
Lane 2 (+): ab178395 & Human placenta lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab178395 in Human placenta lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST.
Datasheets and documents
References
ab240204 has not yet been referenced specifically in any publications.
