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    hgf-antibody-epr12230-bsa-and-azide-free-ab240204.pdf

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RecombinantRabMAb

Recombinant Anti-HGF antibody [EPR12230] - BSA and Azide free (ab240204)

  • Datasheet
  • Certificate of Compliance
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Immunoprecipitation - Anti-HGF antibody [EPR12230] - BSA and Azide free (ab240204)
  • Anti-HGF antibody [EPR12230] - BSA and Azide free (ab240204)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12230] to HGF - BSA and Azide free
  • Suitable for: WB, IP
  • Reacts with: Human

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Recombinant human HGF protein (Active) (ab632)

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Overview

  • Product name

    Anti-HGF antibody [EPR12230] - BSA and Azide free
    See all HGF primary antibodies
  • Description

    Rabbit monoclonal [EPR12230] to HGF - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: IHC-P
  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human HGF aa 100-200 (Cysteine residue). The exact sequence is proprietary.
    Database link: P14210

  • Positive control

    • IP: Human placenta lysate.
  • General notes

    Ab240204 is the carrier-free version of ab178395. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab240204 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR12230
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Growth Factors/Hormones
    • Hormones
    • Cancer
    • Invasion/microenvironment
    • Angiogenesis
    • Angiogenic growth factors

Associated products

  • Alternative Versions

    • Anti-HGF antibody [EPR12230] (ab178395)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant human HGF protein (Active) (ab632)
  • Related Products

    • Recombinant human HGF protein (ab105061)
    • Recombinant Human HGF protein (ab166881)
    • Recombinant human HGF protein (Active) (ab632)

Applications

Our Abpromise guarantee covers the use of ab240204 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 83 kDa.
IP Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function

      Potent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types. Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization.
    • Involvement in disease

      Deafness, autosomal recessive, 39
    • Sequence similarities

      Belongs to the peptidase S1 family. Plasminogen subfamily.
      Contains 4 kringle domains.
      Contains 1 PAN domain.
      Contains 1 peptidase S1 domain.
    • Target information above from: UniProt accession P14210 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 3082 Human
      • Omim: 142409 Human
      • SwissProt: P14210 Human
      • Unigene: 396530 Human
      • Unigene: 561679 Human
      • Alternative names

        • DFNB39 antibody
        • F TCF antibody
        • Fibroblast derived tumor cytotoxic factor antibody
        • Hepatocyte growth factor (hepapoietin A; scatter factor) antibody
        • Hepatocyte growth factor antibody
        • Hepatocyte growth factor beta chain antibody
        • Hepatocyte growth factor precursor antibody
        • Hepatopoietin A antibody
        • Hepatopoietin-A antibody
        • Hgf antibody
        • HGF_HUMAN antibody
        • HGFB antibody
        • HPTA antibody
        • Lung fibroblast derived mitogen antibody
        • OTTHUMP00000161349 antibody
        • OTTHUMP00000206710 antibody
        • OTTHUMP00000206711 antibody
        • OTTHUMP00000206712 antibody
        • OTTHUMP00000206713 antibody
        • OTTHUMP00000206730 antibody
        • Scatter factor antibody
        • SF antibody
        see all

      Images

      • Immunoprecipitation - Anti-HGF antibody [EPR12230] - BSA and Azide free (ab240204)
        Immunoprecipitation - Anti-HGF antibody [EPR12230] - BSA and Azide free (ab240204)

        ab178395 (purified) at 1:30 dilution (2µg) immunoprecipitating HGF in Human placenta lysate.
        Lane 1 (input): Human placenta lysate 10µg
        Lane 2 (+): ab178395 & Human placenta lysate
        Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab178395 in Human placenta lysate
        For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
        Blocking and diluting buffer: 5% NFDM/TBST.

      • Anti-HGF antibody [EPR12230] - BSA and Azide free (ab240204)
        Anti-HGF antibody [EPR12230] - BSA and Azide free (ab240204)

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet

      Certificate of Compliance

      To download a Certificate of Compliance, please enter your Lot number below:

    • References (0)

      Publishing research using ab240204? Please let us know so that we can cite the reference in this datasheet.

      ab240204 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

      Show All Reviews Q&A
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      There are currently no Customer reviews or Questions for ab240204.
      Please use the links above to contact us or submit feedback about this product.

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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