Product nameAnti-HGFA Inhibitor 2 antibody [EPR6283(2)]
See all HGFA Inhibitor 2 primary antibodies
DescriptionRabbit monoclonal [EPR6283(2)] to HGFA Inhibitor 2
Tested applicationsSuitable for: WB, Flow Cyt, ICC/IFmore details
Unsuitable for: IHC-P or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human HGFA Inhibitor 2 aa 100-200 (extracellular). The exact sequence is proprietary.
- Fetal kidney, MCF-7, Human small intestine, Caco-2 and PC-3 lysates; MCF-7 cells.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 7.70 x 10 -11 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab128926 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 34 kDa (predicted molecular weight: 28 kDa).|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|ICC/IF||1/250 - 1/500.|
FunctionInhibitor of HGF activator. Also inhibits plasmin, plasma and tissue kallikrein, and factor XIa.
Tissue specificityExpressed in placenta, kidney, pancreas, prostate, testis, thymus, and trachea.
Involvement in diseaseDefects in SPINT2 are the cause of diarrhea type 3 (DIAR3) [MIM:270420]; also known as congenital sodium diarrhea (CSD). DIAR3 is a rare, inherited diarrhea of infancy. A diagnosis of DIAR3 is made on the findings of a life-threatening secretory diarrhea, severe metabolic acidosis, and hyponatremia secondary to extraordinarily high fecal losses of sodium, with low or normal excretion of urinary sodium, in the absence of infectious, autoimmune, and endocrine causes.
Sequence similaritiesContains 2 BPTI/Kunitz inhibitor domains.
DomainThis inhibitor contains two inhibitory domains.
- Information by UniProt
- AL024025 antibody
- Bikunin, placental antibody
- C76321 antibody
All lanes : Anti-HGFA Inhibitor 2 antibody [EPR6283(2)] (ab128926) at 1/1000 dilution
Lane 1 : Fetal kidney lysate
Lane 2 : MCF-7 cell lysate
Lane 3 : Human small intestine lysate
Lane 4 : Caco-2 lysate
Lane 5 : PC-3 lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 28 kDa
Observed band size: 34 kDa why is the actual band size different from the predicted?
ab128926, at 1/250 dilution, staining HGFA Inhibitor 2 in MCF-7 cells by Immunofluorescence.
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
This product has been referenced in:
- Roversi FM et al. Up-regulation of SPINT2/HAI-2 by Azacytidine in bone marrow mesenchymal stromal cells affects leukemic stem cell survival and adhesion. J Cell Mol Med N/A:N/A (2018). Read more (PubMed: 30484958) »
- Roversi FM et al. Serine protease inhibitor kunitz-type 2 is downregulated in myelodysplastic syndromes and modulates cell-cell adhesion. Stem Cells Dev 23:1109-20 (2014). Read more (PubMed: 24410667) »