Recombinant
RabMAb

Recombinant Anti-HGFA Inhibitor 2 antibody [EPR6283(2)] (ab128926)

Overview

  • Product name

    Anti-HGFA Inhibitor 2 antibody [EPR6283(2)]
    See all HGFA Inhibitor 2 primary antibodies
  • Description

    Rabbit monoclonal [EPR6283(2)] to HGFA Inhibitor 2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cyt, ICC/IFmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human HGFA Inhibitor 2 aa 100-200 (extracellular). The exact sequence is proprietary.

  • Positive control

    • Fetal kidney, MCF-7, Human small intestine, Caco-2 and PC-3 lysates; MCF-7 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab128926 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 34 kDa (predicted molecular weight: 28 kDa).
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

ICC/IF 1/250 - 1/500.
  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function

      Inhibitor of HGF activator. Also inhibits plasmin, plasma and tissue kallikrein, and factor XIa.
    • Tissue specificity

      Expressed in placenta, kidney, pancreas, prostate, testis, thymus, and trachea.
    • Involvement in disease

      Defects in SPINT2 are the cause of diarrhea type 3 (DIAR3) [MIM:270420]; also known as congenital sodium diarrhea (CSD). DIAR3 is a rare, inherited diarrhea of infancy. A diagnosis of DIAR3 is made on the findings of a life-threatening secretory diarrhea, severe metabolic acidosis, and hyponatremia secondary to extraordinarily high fecal losses of sodium, with low or normal excretion of urinary sodium, in the absence of infectious, autoimmune, and endocrine causes.
    • Sequence similarities

      Contains 2 BPTI/Kunitz inhibitor domains.
    • Domain

      This inhibitor contains two inhibitory domains.
    • Cellular localization

      Membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • AL024025 antibody
      • Bikunin, placental antibody
      • C76321 antibody
      • DIAR3 antibody
      • FLJ45571 antibody
      • HAI-2 antibody
      • HAI2 antibody
      • Hepatocyte growth factor activator inhibitor type 2 antibody
      • Kop antibody
      • Kunitz type protease inhibitor 2 antibody
      • Kunitz-type protease inhibitor 2 antibody
      • Kunitz-type serine protease inhibitor antibody
      • MGC72638 antibody
      • PB antibody
      • Placental bikunin antibody
      • Serine peptidase inhibitor Kunitz type 2 antibody
      • Serine protease inhibitor, Kunitz type, 2 antibody
      • SPINT2 antibody
      • SPIT2_HUMAN antibody
      see all

    Images

    • All lanes : Anti-HGFA Inhibitor 2 antibody [EPR6283(2)] (ab128926) at 1/1000 dilution

      Lane 1 : Fetal kidney lysate
      Lane 2 : MCF-7 cell lysate
      Lane 3 : Human small intestine lysate
      Lane 4 : Caco-2 lysate
      Lane 5 : PC-3 lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 28 kDa
      Observed band size: 34 kDa
      why is the actual band size different from the predicted?

    • ab128926, at 1/250 dilution, staining HGFA Inhibitor 2 in MCF-7 cells by Immunofluorescence.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    • Roversi FM  et al. Up-regulation of SPINT2/HAI-2 by Azacytidine in bone marrow mesenchymal stromal cells affects leukemic stem cell survival and adhesion. J Cell Mol Med N/A:N/A (2018). Read more (PubMed: 30484958) »
    • Roversi FM  et al. Serine protease inhibitor kunitz-type 2 is downregulated in myelodysplastic syndromes and modulates cell-cell adhesion. Stem Cells Dev 23:1109-20 (2014). Read more (PubMed: 24410667) »
    See all 2 Publications for this product

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Answer

    Thank you for contacting Abcam.

    Listed in the protocol book that I sent you is the western blot protocol we recommend. Since the HGFA Inhibitor 2 protein is located on the cell membrane, we recommend using a NP-40 or RIPA buffer for cell lysis. Additionally, protease and phosphatase inhibitors should be added fresh to the lysis buffer for each preparation.

    As stated in the protocol, the samples need to be denatured and reduced. Denaturing is done by boiling in SDS at 95-100oC for 5 minutes.

    I've attached the protocol book again, which explains these steps in detail as well as troubleshooting options.

    Here is a link to our western blot guide as well:
    https://www.abcam.com/ps/pdf/protocols/WB-beginner.pdf

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

    Read More

    Answer

    Thank you for contacting Abcam.

    Forwarded below is the information that you told me over the phone regarding ab128926 and ab83056. You mentioned ab128926 did produce a positive signal in the THP cell lysate while ab83056 did not produce a positive signal in the same THP cell lysate. As we discussed, I am sending you a positive control free of charge (Order XXX, ab7914 HL60 whole cell lysate). I expect the whole cell lysate to contain the HGFA Inhibitor 2 protein based on the information I obtained from protein atlas in the following link:
    http://www.proteinatlas.org/ENSG00000167642/cell

    We also discussed some troubleshooting steps to the western blot protocol you were using, specifically for ab83056 due to the presence of non-specific bands and lack of positive bands, listed below:
    1. Lower primary antibody concentration
    2. Load 20ug of positive control rather than 10ug
    3. Block for longer than 1 hour at room temperature using 3% BSA rather than 5% milk

    I also attached our protocol book, which provides further suggestions for troubleshooting when non-specific bands are observed in western blot.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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