Anti-HGS antibody [19-5] (ab150653)
Key features and details
- Mouse monoclonal [19-5] to HGS
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG2b
Overview
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Product name
Anti-HGS antibody [19-5]
See all HGS primary antibodies -
Description
Mouse monoclonal [19-5] to HGS -
Host species
Mouse -
Specificity
The antibody recognizes an epitope is located between the VHS and coiled-coil domain of the HRS protein.
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Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length Human HGS.
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Epitope
The epitope for ab150653 is located between the VHS and coiled-coil domain of HGS. -
Positive control
- Human brain tissue and HeLa lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.20
Constituent: 100% Phosphate Buffer -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Lyophilized protein G purified in PBS pH7.4 -
Clonality
Monoclonal -
Clone number
19-5 -
Isotype
IgG2b -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab150653 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Predicted molecular weight: 86 kDa.
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Notes |
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WB
1/1000. Predicted molecular weight: 86 kDa. |
Target
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Function
Involved in intracellular signal transduction mediated by cytokines and growth factors. When associated with STAM, it suppresses DNA signaling upon stimulation by IL-2 and GM-CSF. Could be a direct effector of PI3-kinase in vesicular pathway via early endosomes and may regulate trafficking to early and late endosomes by recruiting clathrin. May concentrate ubiquitinated receptors within clathrin-coated regions. Involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs) when complexed with STAM (ESCRT-0 complex). The ESCRT-0 complex binds ubiquitin and acts as sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting/trafficking processes. May contribute to the efficient recruitment of SMADs to the activin receptor complex. Involved in receptor recycling via its association with the CART complex, a multiprotein complex required for efficient transferrin receptor recycling but not for EGFR degradation. -
Tissue specificity
Ubiquitous expression in adult and fetal tissues with higher expression in testis and peripheral blood leukocytes. -
Sequence similarities
Contains 1 FYVE-type zinc finger.
Contains 1 UIM (ubiquitin-interacting motif) repeat.
Contains 1 VHS domain. -
Domain
Has a double-sided UIM that can bind 2 ubiquitin molecules, one on each side of the helix.
The FYVE-type zinc finger domain mediates interactions with phosphatidylinositol 3-phosphate in membranes of early endosomes and penetrates bilayers. The FYVE domain insertion into PtdIns(3)P-enriched membranes is substantially increased in acidic conditions. -
Post-translational
modificationsPhosphorylated on Tyr-334. A minor site of phosphorylation on Tyr-329 is detected (By similarity). Phosphorylation occurs in response to EGF, IL-2, GM-CSF and HGF.
Ubiquitinated by ITCH. -
Cellular localization
Cytoplasm. Early endosome membrane. Endosome > multivesicular body membrane. - Information by UniProt
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Database links
- Entrez Gene: 9146 Human
- Omim: 604375 Human
- SwissProt: O14964 Human
- Unigene: 514590 Human
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Alternative names
- Hepatocyte growth factor regulated tyrosine kinase substrate antibody
- Hepatocyte growth factor-regulated tyrosine kinase substrate antibody
- HGF-regulated tyrosine kinase substrate antibody
see all
Images
Protocols
Datasheets and documents
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Datasheet download
References (0)
ab150653 has not yet been referenced specifically in any publications.