Key features and details
- Rabbit polyclonal to HGS
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-HGS antibody
See all HGS primary antibodies
DescriptionRabbit polyclonal to HGS
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
- WB: HEK-293T overexpressing HGS cell lysate. IHC-P: Human testis and cerebral cortex tissue. ICC/IF: U-2 OS cells.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab223753 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 86 kDa.|
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
FunctionInvolved in intracellular signal transduction mediated by cytokines and growth factors. When associated with STAM, it suppresses DNA signaling upon stimulation by IL-2 and GM-CSF. Could be a direct effector of PI3-kinase in vesicular pathway via early endosomes and may regulate trafficking to early and late endosomes by recruiting clathrin. May concentrate ubiquitinated receptors within clathrin-coated regions. Involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs) when complexed with STAM (ESCRT-0 complex). The ESCRT-0 complex binds ubiquitin and acts as sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting/trafficking processes. May contribute to the efficient recruitment of SMADs to the activin receptor complex. Involved in receptor recycling via its association with the CART complex, a multiprotein complex required for efficient transferrin receptor recycling but not for EGFR degradation.
Tissue specificityUbiquitous expression in adult and fetal tissues with higher expression in testis and peripheral blood leukocytes.
Sequence similaritiesContains 1 FYVE-type zinc finger.
Contains 1 UIM (ubiquitin-interacting motif) repeat.
Contains 1 VHS domain.
DomainHas a double-sided UIM that can bind 2 ubiquitin molecules, one on each side of the helix.
The FYVE-type zinc finger domain mediates interactions with phosphatidylinositol 3-phosphate in membranes of early endosomes and penetrates bilayers. The FYVE domain insertion into PtdIns(3)P-enriched membranes is substantially increased in acidic conditions.
modificationsPhosphorylated on Tyr-334. A minor site of phosphorylation on Tyr-329 is detected (By similarity). Phosphorylation occurs in response to EGF, IL-2, GM-CSF and HGF.
Ubiquitinated by ITCH.
Cellular localizationCytoplasm. Early endosome membrane. Endosome > multivesicular body membrane.
- Information by UniProt
- Hepatocyte growth factor regulated tyrosine kinase substrate antibody
- Hepatocyte growth factor-regulated tyrosine kinase substrate antibody
- HGF-regulated tyrosine kinase substrate antibody
Paraffin-embedded human cerebral cortex tissue stained for HGS using ab223753 at 1/50 dilution in immunohistochemical analysis.
All lanes : Anti-HGS antibody (ab223753) at 1/100 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) vector only transfected cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) overexpressing HGS (co-expressed with a C-terminal myc-DDK tag), cell lysate
Developed using the ECL technique.
Predicted band size: 86 kDa
Paraffin-embedded human testis tissue stained for HGS using ab223753 at 1/50 dilution in immunohistochemical analysis.
PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for HGS (green) using ab223753 at 4 µg/ml in ICC/IF.
ab223753 has not yet been referenced specifically in any publications.