Anti-HGS antibody - C-terminal (ab155539)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-HGS antibody - C-terminal
See all HGS primary antibodies -
Description
Rabbit polyclonal to HGS - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: IP, WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment, corresponding to a region within C terminal amino acids 611-777 of Human HGS (UniProt ID: O14964).
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Positive control
- H1299, HeLa, Mouse brain and Rat brain whole cell lysates; Human colon cancer tissue; A431 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab155539 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | 1/500. | |
| WB | 1/1000 - 1/10000. Predicted molecular weight: 86 kDa. | |
| IHC-P | 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| ICC/IF | 1/100 - 1/1000. |
Target
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Function
Involved in intracellular signal transduction mediated by cytokines and growth factors. When associated with STAM, it suppresses DNA signaling upon stimulation by IL-2 and GM-CSF. Could be a direct effector of PI3-kinase in vesicular pathway via early endosomes and may regulate trafficking to early and late endosomes by recruiting clathrin. May concentrate ubiquitinated receptors within clathrin-coated regions. Involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs) when complexed with STAM (ESCRT-0 complex). The ESCRT-0 complex binds ubiquitin and acts as sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting/trafficking processes. May contribute to the efficient recruitment of SMADs to the activin receptor complex. Involved in receptor recycling via its association with the CART complex, a multiprotein complex required for efficient transferrin receptor recycling but not for EGFR degradation. -
Tissue specificity
Ubiquitous expression in adult and fetal tissues with higher expression in testis and peripheral blood leukocytes. -
Sequence similarities
Contains 1 FYVE-type zinc finger.
Contains 1 UIM (ubiquitin-interacting motif) repeat.
Contains 1 VHS domain. -
Domain
Has a double-sided UIM that can bind 2 ubiquitin molecules, one on each side of the helix.
The FYVE-type zinc finger domain mediates interactions with phosphatidylinositol 3-phosphate in membranes of early endosomes and penetrates bilayers. The FYVE domain insertion into PtdIns(3)P-enriched membranes is substantially increased in acidic conditions. -
Post-translational
modificationsPhosphorylated on Tyr-334. A minor site of phosphorylation on Tyr-329 is detected (By similarity). Phosphorylation occurs in response to EGF, IL-2, GM-CSF and HGF.
Ubiquitinated by ITCH. -
Cellular localization
Cytoplasm. Early endosome membrane. Endosome > multivesicular body membrane. - Information by UniProt
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Database links
- Entrez Gene: 9146 Human
- Entrez Gene: 15239 Mouse
- Entrez Gene: 56084 Rat
- Omim: 604375 Human
- SwissProt: O14964 Human
- SwissProt: Q99LI8 Mouse
- SwissProt: Q9JJ50 Rat
- Unigene: 514590 Human
see all -
Alternative names
- Hepatocyte growth factor regulated tyrosine kinase substrate antibody
- Hepatocyte growth factor-regulated tyrosine kinase substrate antibody
- HGF-regulated tyrosine kinase substrate antibody
see all
Images
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Western blot - Anti-HGS antibody - C-terminal (ab155539)All lanes : Anti-HGS antibody - C-terminal (ab155539) at 1/10000 dilution
Lane 1 : H1299 whole cell lysate
Lane 2 : HeLa whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 86 kDa
7.5% SDS PAGE -
Immunoprecipitation - Anti-HGS antibody - C-terminal (ab155539)This image is courtesy of an anonymous Abreviewab155539 immunoprecipitating HGS in human hTERT-RPE1 whole cell lysate. Cell lysate from 4000000 cells was incubated with primary antibody (1/500 in triton 1% buffer) for 12 hours at 4°C. Western blotting confirmed successful immunoprecipation. Lower 50kDa band corresponds to IgG.
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Western blot - Anti-HGS antibody - C-terminal (ab155539)Anti-HGS antibody - C-terminal (ab155539) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg
Predicted band size: 86 kDa
7.5% SDS PAGE -
Western blot - Anti-HGS antibody - C-terminal (ab155539)Anti-HGS antibody - C-terminal (ab155539) at 1/1000 dilution + Rat brain whole cell lysate at 50 µg
Predicted band size: 86 kDa
7.5 % SDS-PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-HGS antibody - C-terminal (ab155539)Confocal immunofluorescence analysis of methanol-fixed A431 cells, labeling HGS with ab155539 at 1/200 dilution (green) and Alpha-tubulin filaments (red). -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HGS antibody - C-terminal (ab155539)Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue, labeling HGS with ab155539 at 1/500 dilution.
Protocols
References
This product has been referenced in:
- Hammerling BC et al. A Rab5 endosomal pathway mediates Parkin-dependent mitochondrial clearance. Nat Commun 8:14050 (2017). Read more (PubMed: 28134239) »
