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    hgs-antibody-c-terminal-ab155539.pdf

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Cell Biology Proteolysis / Ubiquitin Proteasome / Ubiquitin Ubiquitin
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Anti-HGS antibody - C-terminal (ab155539)

  • Datasheet
  • SDS
Reviews (3) Submit a question References (1)

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Western blot - Anti-HGS antibody - C-terminal (ab155539)
  • Western blot - Anti-HGS antibody - C-terminal (ab155539)
  • Western blot - Anti-HGS antibody - C-terminal (ab155539)
  • Immunocytochemistry/ Immunofluorescence - Anti-HGS antibody - C-terminal (ab155539)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HGS antibody - C-terminal (ab155539)

Key features and details

  • Rabbit polyclonal to HGS - C-terminal
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-HGS antibody - C-terminal
    See all HGS primary antibodies
  • Description

    Rabbit polyclonal to HGS - C-terminal
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment, corresponding to a region within C terminal amino acids 611-777 of Human HGS (UniProt ID: O14964).

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Ubiquitin
    • Signal Transduction
    • Protein Phosphorylation
    • Tyrosine Kinases
    • Receptor Tyrosine Kinases

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
    • Mouse brain tissue lysate - total protein (ab30151)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab155539 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
IHC-P
Human
WB
Mouse
Rat
Human
Application Abreviews Notes
WB (1)
1/1000 - 1/10000. Predicted molecular weight: 86 kDa.
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF (1)
1/100 - 1/1000.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 86 kDa.
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/100 - 1/1000.

Target

  • Function

    Involved in intracellular signal transduction mediated by cytokines and growth factors. When associated with STAM, it suppresses DNA signaling upon stimulation by IL-2 and GM-CSF. Could be a direct effector of PI3-kinase in vesicular pathway via early endosomes and may regulate trafficking to early and late endosomes by recruiting clathrin. May concentrate ubiquitinated receptors within clathrin-coated regions. Involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs) when complexed with STAM (ESCRT-0 complex). The ESCRT-0 complex binds ubiquitin and acts as sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting/trafficking processes. May contribute to the efficient recruitment of SMADs to the activin receptor complex. Involved in receptor recycling via its association with the CART complex, a multiprotein complex required for efficient transferrin receptor recycling but not for EGFR degradation.
  • Tissue specificity

    Ubiquitous expression in adult and fetal tissues with higher expression in testis and peripheral blood leukocytes.
  • Sequence similarities

    Contains 1 FYVE-type zinc finger.
    Contains 1 UIM (ubiquitin-interacting motif) repeat.
    Contains 1 VHS domain.
  • Domain

    Has a double-sided UIM that can bind 2 ubiquitin molecules, one on each side of the helix.
    The FYVE-type zinc finger domain mediates interactions with phosphatidylinositol 3-phosphate in membranes of early endosomes and penetrates bilayers. The FYVE domain insertion into PtdIns(3)P-enriched membranes is substantially increased in acidic conditions.
  • Post-translational
    modifications

    Phosphorylated on Tyr-334. A minor site of phosphorylation on Tyr-329 is detected (By similarity). Phosphorylation occurs in response to EGF, IL-2, GM-CSF and HGF.
    Ubiquitinated by ITCH.
  • Cellular localization

    Cytoplasm. Early endosome membrane. Endosome > multivesicular body membrane.
  • Target information above from: UniProt accession O14964 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 9146 Human
    • Entrez Gene: 15239 Mouse
    • Entrez Gene: 56084 Rat
    • Omim: 604375 Human
    • SwissProt: O14964 Human
    • SwissProt: Q99LI8 Mouse
    • SwissProt: Q9JJ50 Rat
    • Unigene: 514590 Human
    • Unigene: 7919 Mouse
    • Unigene: 21 Rat
    see all
  • Alternative names

    • Hepatocyte growth factor regulated tyrosine kinase substrate antibody
    • Hepatocyte growth factor-regulated tyrosine kinase substrate antibody
    • HGF-regulated tyrosine kinase substrate antibody
    • HGNC:4897 antibody
    • HGS antibody
    • HGS_HUMAN antibody
    • Hrs antibody
    • Human growth factor regulated tyrosine kinase substrate antibody
    • Protein pp110 antibody
    • ZFYVE8 antibody
    see all

Images

  • Western blot - Anti-HGS antibody - C-terminal (ab155539)
    Western blot - Anti-HGS antibody - C-terminal (ab155539)
    All lanes : Anti-HGS antibody - C-terminal (ab155539) at 1/10000 dilution

    Lane 1 : H1299 whole cell lysate
    Lane 2 : HeLa whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 86 kDa



    7.5% SDS PAGE
  • Western blot - Anti-HGS antibody - C-terminal (ab155539)
    Western blot - Anti-HGS antibody - C-terminal (ab155539)
    Anti-HGS antibody - C-terminal (ab155539) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg

    Predicted band size: 86 kDa



    7.5% SDS PAGE
  • Western blot - Anti-HGS antibody - C-terminal (ab155539)
    Western blot - Anti-HGS antibody - C-terminal (ab155539)
    Anti-HGS antibody - C-terminal (ab155539) at 1/1000 dilution + Rat brain whole cell lysate at 50 µg

    Predicted band size: 86 kDa



    7.5 % SDS-PAGE
  • Immunocytochemistry/ Immunofluorescence - Anti-HGS antibody - C-terminal (ab155539)
    Immunocytochemistry/ Immunofluorescence - Anti-HGS antibody - C-terminal (ab155539)
    Confocal immunofluorescence analysis of methanol-fixed A431 cells, labeling HGS with ab155539 at 1/200 dilution (green) and Alpha-tubulin filaments (red).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HGS antibody - C-terminal (ab155539)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HGS antibody - C-terminal (ab155539)
    Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue, labeling HGS with ab155539 at 1/500 dilution.

Protocols

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (1)

    Publishing research using ab155539? Please let us know so that we can cite the reference in this datasheet.

    ab155539 has been referenced in 1 publication.

    • Hammerling BC  et al. A Rab5 endosomal pathway mediates Parkin-dependent mitochondrial clearance. Nat Commun 8:14050 (2017). PubMed: 28134239

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-3 of 3 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-HGS antibody - C-terminal

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (hTERT-RPE1 cells)
    Specification
    hTERT-RPE1 cells
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - saponin
    Blocking step
    BSA as blocking agent for 20 minute(s) · Concentration: 0.02% · Temperature: 18°C
    Read More

    Abcam user community

    Verified customer

    Submitted May 09 2013

    Immunoprecipitation abreview for Anti-HGS antibody - C-terminal

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunoprecipitation
    Sample
    Human Cell lysate - whole cell (hTERT-RPE1 cells)
    Total protein in input
    4e+006 cells
    Specification
    hTERT-RPE1 cells
    Immuno-precipitation step
    Protein A/G
    Read More

    Abcam user community

    Verified customer

    Submitted May 07 2013

    Western blot abreview for Anti-HGS antibody - C-terminal

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (hTERT-RPE1 cells)
    Loading amount
    5000 cells
    Specification
    hTERT-RPE1 cells
    Gel Running Conditions
    Reduced Denaturing (10% acrylamide)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 18°C
    Read More

    Abcam user community

    Verified customer

    Submitted Apr 09 2013

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