Anti-HGS antibody - C-terminal (ab155539)
Key features and details
- Rabbit polyclonal to HGS - C-terminal
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-HGS antibody - C-terminal
See all HGS primary antibodies -
Description
Rabbit polyclonal to HGS - C-terminal -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P HumanWB MouseRatHuman
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Immunogen
Recombinant fragment, corresponding to a region within C terminal amino acids 611-777 of Human HGS (UniProt ID: O14964).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab155539 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Guaranteed
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted
Predicted to work for this combination of applications and species but not guaranteed.
Incompatible
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| ICC/IF |
Human
|
| IHC-P |
Human
|
| WB |
Mouse
Rat
Human
|
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 86 kDa.
|
| IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
| ICC/IF | (1) |
1/100 - 1/1000.
|
| Notes |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 86 kDa. |
|
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
ICC/IF
1/100 - 1/1000. |
Target
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Function
Involved in intracellular signal transduction mediated by cytokines and growth factors. When associated with STAM, it suppresses DNA signaling upon stimulation by IL-2 and GM-CSF. Could be a direct effector of PI3-kinase in vesicular pathway via early endosomes and may regulate trafficking to early and late endosomes by recruiting clathrin. May concentrate ubiquitinated receptors within clathrin-coated regions. Involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs) when complexed with STAM (ESCRT-0 complex). The ESCRT-0 complex binds ubiquitin and acts as sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting/trafficking processes. May contribute to the efficient recruitment of SMADs to the activin receptor complex. Involved in receptor recycling via its association with the CART complex, a multiprotein complex required for efficient transferrin receptor recycling but not for EGFR degradation. -
Tissue specificity
Ubiquitous expression in adult and fetal tissues with higher expression in testis and peripheral blood leukocytes. -
Sequence similarities
Contains 1 FYVE-type zinc finger.
Contains 1 UIM (ubiquitin-interacting motif) repeat.
Contains 1 VHS domain. -
Domain
Has a double-sided UIM that can bind 2 ubiquitin molecules, one on each side of the helix.
The FYVE-type zinc finger domain mediates interactions with phosphatidylinositol 3-phosphate in membranes of early endosomes and penetrates bilayers. The FYVE domain insertion into PtdIns(3)P-enriched membranes is substantially increased in acidic conditions. -
Post-translational
modificationsPhosphorylated on Tyr-334. A minor site of phosphorylation on Tyr-329 is detected (By similarity). Phosphorylation occurs in response to EGF, IL-2, GM-CSF and HGF.
Ubiquitinated by ITCH. -
Cellular localization
Cytoplasm. Early endosome membrane. Endosome > multivesicular body membrane. - Information by UniProt
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Database links
- Entrez Gene: 9146 Human
- Entrez Gene: 15239 Mouse
- Entrez Gene: 56084 Rat
- Omim: 604375 Human
- SwissProt: O14964 Human
- SwissProt: Q99LI8 Mouse
- SwissProt: Q9JJ50 Rat
- Unigene: 514590 Human
see all -
Alternative names
- Hepatocyte growth factor regulated tyrosine kinase substrate antibody
- Hepatocyte growth factor-regulated tyrosine kinase substrate antibody
- HGF-regulated tyrosine kinase substrate antibody
see all
Images
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Western blot - Anti-HGS antibody - C-terminal (ab155539)All lanes : Anti-HGS antibody - C-terminal (ab155539) at 1/10000 dilution
Lane 1 : H1299 whole cell lysate
Lane 2 : HeLa whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 86 kDa
7.5% SDS PAGE -
Western blot - Anti-HGS antibody - C-terminal (ab155539)Anti-HGS antibody - C-terminal (ab155539) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg
Predicted band size: 86 kDa
7.5% SDS PAGE -
Western blot - Anti-HGS antibody - C-terminal (ab155539)Anti-HGS antibody - C-terminal (ab155539) at 1/1000 dilution + Rat brain whole cell lysate at 50 µg
Predicted band size: 86 kDa
7.5 % SDS-PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-HGS antibody - C-terminal (ab155539)Confocal immunofluorescence analysis of methanol-fixed A431 cells, labeling HGS with ab155539 at 1/200 dilution (green) and Alpha-tubulin filaments (red). -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HGS antibody - C-terminal (ab155539)Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue, labeling HGS with ab155539 at 1/500 dilution.
Protocols
References (1)
ab155539 has been referenced in 1 publication.
- Hammerling BC et al. A Rab5 endosomal pathway mediates Parkin-dependent mitochondrial clearance. Nat Commun 8:14050 (2017). PubMed: 28134239
