1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/100 - 1/1000.
Involved in intracellular signal transduction mediated by cytokines and growth factors. When associated with STAM, it suppresses DNA signaling upon stimulation by IL-2 and GM-CSF. Could be a direct effector of PI3-kinase in vesicular pathway via early endosomes and may regulate trafficking to early and late endosomes by recruiting clathrin. May concentrate ubiquitinated receptors within clathrin-coated regions. Involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs) when complexed with STAM (ESCRT-0 complex). The ESCRT-0 complex binds ubiquitin and acts as sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting/trafficking processes. May contribute to the efficient recruitment of SMADs to the activin receptor complex. Involved in receptor recycling via its association with the CART complex, a multiprotein complex required for efficient transferrin receptor recycling but not for EGFR degradation.
Ubiquitous expression in adult and fetal tissues with higher expression in testis and peripheral blood leukocytes.
Has a double-sided UIM that can bind 2 ubiquitin molecules, one on each side of the helix. The FYVE-type zinc finger domain mediates interactions with phosphatidylinositol 3-phosphate in membranes of early endosomes and penetrates bilayers. The FYVE domain insertion into PtdIns(3)P-enriched membranes is substantially increased in acidic conditions.
Phosphorylated on Tyr-334. A minor site of phosphorylation on Tyr-329 is detected (By similarity). Phosphorylation occurs in response to EGF, IL-2, GM-CSF and HGF. Ubiquitinated by ITCH.
Cytoplasm. Early endosome membrane. Endosome > multivesicular body membrane.
Human growth factor regulated tyrosine kinase substrate antibody
Protein pp110 antibody
Immunoprecipitation - Anti-HGS antibody - C-terminal (ab155539)This image is courtesy of an anonymous Abreview
ab155539 immunoprecipitating HGS in human hTERT-RPE1 whole cell lysate. Cell lysate from 4000000 cells was incubated with primary antibody (1/500 in triton 1% buffer) for 12 hours at 4°C. Western blotting confirmed successful immunoprecipation. Lower 50kDa band corresponds to IgG.