Key features and details
- Rabbit polyclonal to Hhip
- Suitable for: IHC-P, WB
- Reacts with: Rat, Human
- Isotype: IgG
Product nameAnti-Hhip antibody
See all Hhip primary antibodies
DescriptionRabbit polyclonal to Hhip
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Rat, Human
Recombinant fragment corresponding to Human Hhip aa 18-220.
FFEGDAKFGERNEGSGARRRRCLNGNPPKRLKRRDRRMMSQLELLSGGEM LCGGFYPRLSCCLRSDSPGLGRLENKIFSVTNNTECGKLLEEIKCALCSP HSQSLFHSPEREVLERDLVLPLLCKDYCKEFFYTCRGHIPGFLQTTADEF CFYYARKDGGLCFPDFPRKQVRGPASNYLDQMEEYDKVEEISRKHKHNCF CIQ
Database link: Q96QV1
- WB: Rat heart tissue lysate. IHC-P: Human testis and brain tissue.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab230271 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
|WB||1/1000 - 1/5000. Detects a band of approximately 79 kDa (predicted molecular weight: 79, 36 kDa).|
FunctionModulates hedgehog signaling in several cell types including brain and lung through direct interaction with members of the hedgehog family.
Tissue specificityWidely expressed in fetal and adult tissues. Highest expression in adult heart, liver and pancreas, and in fetal kidney.
Sequence similaritiesBelongs to the HHIP family.
Contains 2 EGF-like domains.
DomainA flexible loop interacts with the SHH zinc binding site and contributes to zinc binding.
Cellular localizationCytoplasm and Cell membrane. Secreted. The last 22 C-terminal amino acids may participate in cell membrane attachment.
- Information by UniProt
- FLJ20992 antibody
- FLJ90230 antibody
- Hedgehog interacting protein antibody
Anti-Hhip antibody (ab230271) at 1/1000 dilution + Rat heart tissue lysate
Goat polyclonal to rabbit IgG at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 79, 36 kDa
Observed band size: 79 kDa why is the actual band size different from the predicted?
Paraffin-embedded human testis tissue stained for Hhip with ab230271 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human brain tissue stained for Hhip with ab230271 at 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab230271 has not yet been referenced specifically in any publications.