• Product name

  • Description

    Rabbit polyclonal to Hhip
  • Host species

  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Cow
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human Hhip.

    Read Abcam's proprietary immunogen policy (Peptide available as ab39947.)

  • Positive control

    • This antibody gave a positive signal in the following lysates: HeLa Whole Cell; Mouse Heart; Mouse Liver; Mouse Pancreas.



Our Abpromise guarantee covers the use of ab39208 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 79 kDa).


  • Function

    Modulates hedgehog signaling in several cell types including brain and lung through direct interaction with members of the hedgehog family.
  • Tissue specificity

    Widely expressed in fetal and adult tissues. Highest expression in adult heart, liver and pancreas, and in fetal kidney.
  • Sequence similarities

    Belongs to the HHIP family.
    Contains 2 EGF-like domains.
  • Domain

    A flexible loop interacts with the SHH zinc binding site and contributes to zinc binding.
  • Cellular localization

    Cytoplasm and Cell membrane. Secreted. The last 22 C-terminal amino acids may participate in cell membrane attachment.
  • Information by UniProt
  • Database links

  • Alternative names

    • FLJ20992 antibody
    • FLJ90230 antibody
    • Hedgehog interacting protein antibody
    • Hedgehog-interacting protein antibody
    • hedgehoginteracting protein antibody
    • HHIP antibody
    • HHIP_HUMAN antibody
    • Hip antibody
    see all


  • All lanes : Anti-Hhip antibody (ab39208) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Heart (Mouse) Tissue Lysate
    Lane 3 : Liver (Mouse) Tissue Lysate
    Lane 4 : Pancreas (Mouse) Tissue Lysate
    Lane 5 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate with Human Hhip peptide (ab39947) at 1 µg/ml
    Lane 6 : Heart (Mouse) Tissue Lysate with Human Hhip peptide (ab39947) at 1 µg/ml
    Lane 7 : Liver (Mouse) Tissue Lysate with Human Hhip peptide (ab39947) at 1 µg/ml
    Lane 8 : Pancreas (Mouse) Tissue Lysate with Human Hhip peptide (ab39947) at 1 µg/ml

    Lysates/proteins at 10 µg per lane.

    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 79 kDa
    Observed band size: 80 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 100 kDa, 45 kDa, 47 kDa. We are unsure as to the identity of these extra bands.

    Exposure time: 5 minutes


This product has been referenced in:

  • Lee BNR  et al. Hedgehog-Interacting Protein (HIP) Regulates Apoptosis Evasion and Angiogenic Function of Late Endothelial Progenitor Cells. Sci Rep 7:12449 (2017). WB ; Mouse . Read more (PubMed: 28963460) »
  • Wang C  et al. EZH2 Mediates epigenetic silencing of neuroblastoma suppressor genes CASZ1, CLU, RUNX3, and NGFR. Cancer Res 72:315-24 (2012). Read more (PubMed: 22068036) »
See all 4 Publications for this product

Customer reviews and Q&As

Western blot
Human Cell lysate - whole cell (various incl: HeLa Ms Liver Ms Kidney)
Loading amount
30 µg
various incl: HeLa Ms Liver Ms Kidney
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

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Submitted Nov 14 2008

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