Anti-Hhip antibody (ab39208)
Key features and details
- Rabbit polyclonal to Hhip
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Hhip antibody
See all Hhip primary antibodies -
Description
Rabbit polyclonal to Hhip -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Cow -
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human Hhip.
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Positive control
- This antibody gave a positive signal in the following lysates: HeLa Whole Cell; Mouse Heart; Mouse Liver; Mouse Pancreas.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab39208 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 79 kDa).
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Notes |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 79 kDa). |
Target
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Function
Modulates hedgehog signaling in several cell types including brain and lung through direct interaction with members of the hedgehog family. -
Tissue specificity
Widely expressed in fetal and adult tissues. Highest expression in adult heart, liver and pancreas, and in fetal kidney. -
Sequence similarities
Belongs to the HHIP family.
Contains 2 EGF-like domains. -
Domain
A flexible loop interacts with the SHH zinc binding site and contributes to zinc binding. -
Cellular localization
Cytoplasm and Cell membrane. Secreted. The last 22 C-terminal amino acids may participate in cell membrane attachment. - Information by UniProt
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Database links
- Entrez Gene: 540830 Cow
- Entrez Gene: 64399 Human
- Entrez Gene: 15245 Mouse
- Omim: 606178 Human
- SwissProt: Q96QV1 Human
- SwissProt: Q7TN16 Mouse
- Unigene: 507991 Human
- Unigene: 254493 Mouse
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Alternative names
- FLJ20992 antibody
- FLJ90230 antibody
- Hedgehog interacting protein antibody
see all
Images
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All lanes : Anti-Hhip antibody (ab39208) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Heart (Mouse) Tissue Lysate
Lane 3 : Liver (Mouse) Tissue Lysate
Lane 4 : Pancreas (Mouse) Tissue Lysate
Lane 5 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate with Human Hhip peptide (ab39947) at 1 µg/ml
Lane 6 : Heart (Mouse) Tissue Lysate with Human Hhip peptide (ab39947) at 1 µg/ml
Lane 7 : Liver (Mouse) Tissue Lysate with Human Hhip peptide (ab39947) at 1 µg/ml
Lane 8 : Pancreas (Mouse) Tissue Lysate with Human Hhip peptide (ab39947) at 1 µg/ml
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 79 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?
Additional bands at: 100 kDa, 45 kDa, 47 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 5 minutes
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab39208 has been referenced in 5 publications.
- Qiu S et al. LncRNA EGOT decreases breast cancer cell viability and migration via inactivation of the Hedgehog pathway. FEBS Open Bio 10:817-826 (2020). PubMed: 32150666
- Lee BNR et al. Hedgehog-Interacting Protein (HIP) Regulates Apoptosis Evasion and Angiogenic Function of Late Endothelial Progenitor Cells. Sci Rep 7:12449 (2017). WB ; Mouse . PubMed: 28963460
- Wang C et al. EZH2 Mediates epigenetic silencing of neuroblastoma suppressor genes CASZ1, CLU, RUNX3, and NGFR. Cancer Res 72:315-24 (2012). PubMed: 22068036
- Zhou X et al. Identification of a chronic obstructive pulmonary disease genetic determinant that regulates HHIP. Hum Mol Genet 21:1325-35 (2012). WB ; Human . PubMed: 22140090
- Angeloni NL et al. The role of hedgehog-interacting protein in maintaining cavernous nerve integrity and adult penile morphology. J Sex Med 6:2480-93 (2009). IHC-Fr ; Rat . PubMed: 19515211