Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23563-120] to HHLA2
- Suitable for: Flow Cyt
- Reacts with: Human
Product nameAnti-HHLA2 antibody [EPR23563-120]
See all HHLA2 primary antibodies
DescriptionRabbit monoclonal [EPR23563-120] to HHLA2
Tested applicationsSuitable for: Flow Cytmore details
Unsuitable for: ICC,IHC-P,IP or WB
Species reactivityReacts with: Human
Recombinant fragment within Human HHLA2 aa 1-350. The exact sequence is proprietary.
Database link: Q9UM44
- Flow cyt: A-204 cells; HEK-293T cells transfected with Myc-His tagged HHLA2 overexpression vector.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab254367 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tissue specificityExpressed in colon, small intestine, lung and kidney.
Sequence similaritiesContains 1 Ig-like C1-type (immunoglobulin-like) domain.
Contains 2 Ig-like V-type (immunoglobulin-like) domains.
- Information by UniProt
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Flow cytometric analysis of HeLa (human cervix adenocarcinoma epithelial cell line, left) / A-204 (human muscle rhabdomyosarcoma, right) cells labelling HHLA2 with ab254367 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
Negative control: Hela (PMID:23716685).
Gated on viable cells.
Flow cytometric analysis of 2% paraformaldehyde fixed, 0.1% Tween-20 permeabilized HEK-293T (Human embryonic kidney epithelial cell line) cells transfected with Myc-His tagged HHLA2 overexpression vector labelling HHLA2 with ab254367 at 1/500 dilution (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left).
A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
Cells were surface stained with rabbit IgG or ab254367. Then fixed with 2% PFA for 10min followed by intracellularly stained with anti-myc tag conjugated to Alexa Fluor®647.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab254367 has not yet been referenced specifically in any publications.