Product nameAnti-Hho1 antibody
DescriptionRabbit polyclonal to Hho1
SpecificityThis antibody recognizes linker histone from S. Cerevisiae.
Tested applicationsSuitable for: ChIP, CHIPseq, WBmore details
Species reactivityReacts with: Saccharomyces cerevisiae
Recombinant full length protein purified from E. coli.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: 50% Glycerol, Whole serum
Our Abpromise guarantee covers the use of ab71833 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ChIP||Use at an assay dependent concentration. PubMed: 22689825|
|CHIPseq||Use at an assay dependent concentration. PubMed: 22689825|
|WB||1/1000. Predicted molecular weight: 28 kDa.|
RelevanceHho1 could act as an H1-type linker histone. It has been shown to bind DNA.
- Hho1p antibody
- Histone H1, a linker histone required for nucleosome packaging at restricted sites antibody
- LPI17C antibody
1: 50 ng recombinant Hho1.
2: WCE from Hho1 knockout yeast expressing deletion construct A.
3: WCE from Hho1 knockout yeast expressing deletion construct B.
4: WCE from Hho1 knockout yeast expressing deletion construct C.
5: WCE from Hho1 knockout yeast expressing deletion construct D.
6: WCE from Hho1 knockout yeast expressing deletion construct E.
7: WCE from hho1 knockout yeast expressing full-length Hho1(F).
8: WCE from hho1 knockout yeast carrying an empty vector.
9: WCE from hho1 knockout yeast.
10: WCE from wild-type yeast.
All lanes : Anti-Hho1 antibody (ab71833) at 1/1000 dilution
Lane 1 : Wild type S. cerevisiae whole cell lysate
Lane 2 : Hho1 knock-out S. cerevisiae whole cell lysate
Lane 3 : S. cerevisiae whole cell lysate expressing Hho1 with tag
Lysates/proteins at 15 µg per lane.
All lanes : HRP-conjugated goat anti-rabbit polyclonal IgG at 1/5000 dilution
Predicted band size: 28 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
This product has been referenced in:
- Lawrence MBD et al. Histone Acetylation, Not Stoichiometry, Regulates Linker Histone Binding in Saccharomyces cerevisiae. Genetics 207:347-355 (2017). Read more (PubMed: 28739661) »
- Brush GS Evidence that histone H1 is dispensable for proper meiotic recombination in budding yeast. BMC Res Notes 8:275 (2015). Read more (PubMed: 26122007) »