Overview

  • Product name

    Anti-hHR23A antibody [EPR4818]
    See all hHR23A primary antibodies
  • Description

    Rabbit monoclonal [EPR4818] to hHR23A
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human hHR23A aa 200-300. The exact sequence is proprietary.

  • Positive control

    • MCF7, HeLa, Raji, and Jurkat cell lysates; Jurkat cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.40
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR4818
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab108592 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 40 kDa.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC,IHC-P or IP.
  • Target

    • Function

      Multiubiquitin chain receptor involved in modulation of proteasomal degradation. Binds to 'Lys-48'-linked polyubiquitin chains in a length-dependent manner and with a lower affinity to 'Lys-63'-linked polyubiquitin chains. Proposed to be capable to bind simultaneously to the 26S proteasome and to polyubiquitinated substrates and to deliver ubiquitinated proteins to the proteasome.
      Involved in nucleotide excision repair and is thought to be functional equivalent for RAD23B in global genome nucleotide excision repair (GG-NER) by association with XPC. In vitro, the XPC:RAD23A dimer has NER activity. Can stabilize XPC.
      Involved in vpr-dependent replication of HIV-1 in non-proliferating cells and primary macrophages. Required for the association of HIV-1 vpr with the host proteasome.
    • Sequence similarities

      Belongs to the RAD23 family.
      Contains 2 UBA domains.
      Contains 1 ubiquitin-like domain.
    • Domain

      The ubiquitin-like domain mediates interaction with ATXN3.
      The ubiquitin-like (UBL) and the UBA (ubiquitin-associated) domains interact intramolecularly in a highly dynamic manner, as each UBA domain competes for an overlapping UBL domain surface. Binding of ubiquitin or proteasome subunit PSMD4 disrupt the UBL-UBA domain interactions and drive RAD23A in to an open conformation.
    • Post-translational
      modifications

      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization

      Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • hHR 23A antibody
      • hHR23A antibody
      • HR23A antibody
      • MGC111083 antibody
      • RAD 23a antibody
      • RAD23 homolog A (S. cerevisiae) antibody
      • RAD23 homolog A antibody
      • RAD23 yeast homolog A antibody
      • RAD23A antibody
      • RD23A_HUMAN antibody
      • UV excision repair protein RAD23 antibody
      • UV excision repair protein RAD23 homolog A antibody
      see all

    Images

    • All lanes : Anti-hHR23A antibody [EPR4818] (ab108592) at 1/10000 dilution

      Lane 1 : MCF7 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : Raji cell lysate
      Lane 4 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 40 kDa

    • ab108592 at 1/10 dilution staining hHR23A in permeabilized Jurkat cells by Flow cytometry (shown in red). Rabbit IgG (negative) in green.

    References

    This product has been referenced in:

    • Ren B  et al. Comparative proteomics reveals the neurotoxicity mechanism of ER stressors tunicamycin and dithiothreitol. Neurotoxicology 68:25-37 (2018). Read more (PubMed: 30003905) »
    See 1 Publication for this product

    Customer reviews and Q&As

    1-3 of 3 Abreviews or Q&A

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Permeabilization
    Yes - 0.5% Triton X-100
    Specification
    HeLa
    Fixative
    Paraformaldehyde

    Dr. Kirk Mcmanus

    Verified customer

    Submitted Jul 03 2018

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (u2os)
    Specification
    u2os
    Permeabilization
    No
    Fixative
    Formaldehyde

    Abcam user community

    Verified customer

    Submitted Jan 02 2014

    Application
    Western blot
    Loading amount
    30 µg
    Gel Running Conditions
    Reduced Denaturing (12%)
    Sample
    Human Cell lysate - whole cell (U2OS)
    Specification
    U2OS
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted Jan 02 2014

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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