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    hhr23b-antibody-ab125918.pdf

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Epigenetics and Nuclear Signaling DNA / RNA DNA Damage & Repair Nucl. Excision Repair
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Anti-hHR23b antibody (ab125918)

  • Datasheet
  • SDS
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Western blot - Anti-hHR23b antibody (ab125918)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hHR23b antibody (ab125918)

Key features and details

  • Rabbit polyclonal to hHR23b
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

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Secondary
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Protein
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Recombinant Human hHR23b protein (ab205997)

View more associated products

Overview

  • Product name

    Anti-hHR23b antibody
    See all hHR23b primary antibodies
  • Description

    Rabbit polyclonal to hHR23b
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-298 of the Human hHR23b protein (P54727).

  • Positive control

    • 293T, A431, Jurkat and Raji cell lysates and Human OVCA tissues.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • Nucl. Excision Repair

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • Jurkat cytoplasmic extract lysate (ab14842)
    • Raji whole cell lysate (ab30124)
    • A-431 whole cell lysate (ab30132)
    • Jurkat whole cell lysate (ab7899)
    • A-431 whole cell lysate (ab7909)
  • Recombinant Protein

    • Recombinant Human hHR23b protein (ab205997)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab125918 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Human
WB
Human
All applications
Mouse
Rat
Cow
Application Abreviews Notes
WB
1/5000 - 1/20000. Predicted molecular weight: 43 kDa.
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. citrate buffe-10mM
Notes
WB
1/5000 - 1/20000. Predicted molecular weight: 43 kDa.
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. citrate buffe-10mM

Target

  • Function

    Multiubiquitin chain receptor involved in modulation of proteasomal degradation. Binds to polyubiquitin chains. Proposed to be capable to bind simultaneously to the 26S proteasome and to polyubiquitinated substrates and to deliver ubiquitinated proteins to the proteasome. May play a role in endoplasmatic reticulum-associated degradation (ERAD) of misfolded glycoproteins by association with PNGase and delivering deglycosylated proteins to the proteasome.
    Involved in global genome nucleotide excision repair (GG-NER) by acting as component of the XPC complex. Cooperatively with CETN2 appears to stabilize XPC. May protect XPC from proteasomal degradation.
    The XPC complex is proposed to represent the first factor bound at the sites of DNA damage and together with other core recognition factors, XPA, RPA and the TFIIH complex, is part of the pre-incision (or initial recognition) complex. The XPC complex recognizes a wide spectrum of damaged DNA characterized by distortions of the DNA helix such as single-stranded loops, mismatched bubbles or single stranded overhangs. The orientation of XPC complex binding appears to be crucial for inducing a productive NER. XPC complex is proposed to recognize and to interact with unpaired bases on the undamaged DNA strand which is followed by recruitment of the TFIIH complex and subsequent scanning for lesions in the opposite strand in a 5'-to-3' direction by the NER machinery. Cyclobutane pyrimidine dimers (CPDs) which are formed upon UV-induced DNA damage esacpe detection by the XPC complex due to a low degree of structural perurbation. Instead they are detected by the UV-DDB complex which in turn recruits and cooperates with the XPC complex in the respective DNA repair. In vitro, the XPC:RAD23B dimer is sufficient to initiate NER; it preferentially binds to cisplatin and UV-damaged double-stranded DNA and also binds to a variety of chemically and structurally diverse DNA adducts. XPC:RAD23B contacts DNA both 5' and 3' of a cisplatin lesion with a preference for the 5' side. XPC:RAD23B induces a bend in DNA upon binding. XPC:RAD23B stimulates the activity of DNA glycosylases TDG and SMUG1.
  • Sequence similarities

    Belongs to the RAD23 family.
    Contains 1 STI1 domain.
    Contains 2 UBA domains.
    Contains 1 ubiquitin-like domain.
  • Domain

    The ubiquitin-like domain mediates interaction with ATXN3.
  • Cellular localization

    Nucleus. Cytoplasm. The intracellular distribution is cell cycle dependent. Localized to the nucleus and the cytoplasm during G1 phase. Nuclear levels decrease during S-phase; upon entering mitosis, relocalizes in the cytoplasm without association with chromatin.
  • Target information above from: UniProt accession P54727 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 530189 Cow
    • Entrez Gene: 5887 Human
    • Entrez Gene: 19359 Mouse
    • Entrez Gene: 298012 Rat
    • Omim: 600062 Human
    • SwissProt: Q29RK4 Cow
    • SwissProt: P54727 Human
    • SwissProt: P54728 Mouse
    • SwissProt: Q4KMA2 Rat
    • Unigene: 521640 Human
    • Unigene: 196846 Mouse
    • Unigene: 67042 Rat
    see all
  • Alternative names

    • hHR 23b antibody
    • hHR23B antibody
    • HR 23B antibody
    • HR23B antibody
    • mHR 23B antibody
    • mHR23B antibody
    • p58 antibody
    • RAD 23B antibody
    • RAD23 (S. cerevisiae) homolog B antibody
    • RAD23 homolog B (S. cerevisiae) antibody
    • RAD23 homolog B antibody
    • RAD23 yeast homolog of B antibody
    • Rad23b antibody
    • RD23B_HUMAN antibody
    • UV excision repair protein RAD23 homolog B antibody
    • XP C repair complementing complex 58 kDa antibody
    • XP C repair complementing complex 58 kDa protein antibody
    • XP C repair complementing protein antibody
    • XP-C repair-complementing complex 58 kDa protein antibody
    • XPC repair complementing complex 58 kDa antibody
    • XPC repair complementing complex 58 kDa protein antibody
    • XPC repair complementing protein antibody
    see all

Images

  • Western blot - Anti-hHR23b antibody (ab125918)
    Western blot - Anti-hHR23b antibody (ab125918)
    All lanes : Anti-hHR23b antibody (ab125918) at 1/10000 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : A431 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 43 kDa



    Gel concentration:12%
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hHR23b antibody (ab125918)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hHR23b antibody (ab125918)
    ab125918, at 1/250, staining hHR23b in Human OVCA tissue by immunohistochemistry [Paraffin Embedded Tissues (IHC-P)].

Protocols

  • Western blot protocols
  • Immunohistochemistry protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (0)

    Publishing research using ab125918? Please let us know so that we can cite the reference in this datasheet.

    ab125918 has not yet been referenced specifically in any publications.

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