Western blot abreview for Anti-hHR23b antibody

Average
Abreviews
Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (12%)
Sample
Human Cell lysate - whole cell (U2OS)
Specification
U2OS

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% BSA and 0.02% azide in TTBS buffer
Dilution
1/500

Secondary antibody

Name
Non-Abcam antibody was used: HRP Goat Anti-Mouse IgG (H+L)
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/20000

Detection

Detection method
ECL
Bands
Specific: 60 kDa Non-specific: 50 kDa
Exposure
1 minute(s) and 0 second(s)

Additional data

Additional Notes
Cells were transfected with siRNA against RAD23B and harvested at different time points after siRNA transfection (48, 72 and 96hr) non-specific siRNA (siLUC) was used as a control

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Submitted Jan 06 2014

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