siRNA against RAD23B was used to check for the antibody specificity.
Non specific siRNA (siLUC) was used as a negative control.
The staining is not specific to RAD23B!!!!
We appreciate the valuable feedback provided in this Abreview. The Western Blot data provided by the same customer (Review ID 40359) shows that ab88503 seems to detect an additional band which cannot be reduced after siRNA treatment. We are unsure about the identity of this band. However, this might explain the staining for cells as shown in this Review. We are therefore investigating if this antibody is suitable for ICC/IF and IHC-P applications.
Abcam user community
Submitted Jan 06 2014
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