Immunocytochemistry/ Immunofluorescence abreview for Anti-hHR23b antibody

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Abreviews
This product is known to not work in this application or species.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (u2os)
Specification
u2os
Fixative
Paraformaldehyde

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: antibody diluent solution
Dilution
1/100

Secondary antibody

Name
Non-Abcam antibody was used: Alexa Fluor 568 donkey anti-mouse
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor­ 568
Dilution
1/500

Additional data

Additional Notes
siRNA against RAD23B was used to check for the antibody specificity. Non specific siRNA (siLUC) was used as a negative control. The staining is not specific to RAD23B!!!!
Abcam response
We appreciate the valuable feedback provided in this Abreview. The Western Blot data provided by the same customer (Review ID 40359) shows that ab88503 seems to detect an additional band which cannot be reduced after siRNA treatment. We are unsure about the identity of this band. However, this might explain the staining for cells as shown in this Review. We are therefore investigating if this antibody is suitable for ICC/IF and IHC-P applications.

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Submitted Jan 06 2014

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