Key features and details
- Mouse monoclonal  to HHV6AgQ1 - BSA and Azide free
- Suitable for: ELISA, WB, ICC/IF, IP, Flow Cyt
- Reacts with: Human herpesvirus
- Isotype: IgG
Product nameAnti-HHV6AgQ1 antibody  - BSA and Azide free
DescriptionMouse monoclonal  to HHV6AgQ1 - BSA and Azide free
Specificityab179902 reacts with gQ1 of HHV6A and HHV6B. This antibody is not recommended for IP of HHV-6B.
Tested applicationsSuitable for: ELISA, WB, ICC/IF, IP, Flow Cytmore details
Species reactivityReacts with: Human herpesvirus
Recombinant fragment (His-tag) corresponding to Human Herpesvirus HHV6AgQ1 aa 3-422. (Expressed in E. coli).
TARLSAMKPPRSCALIFLCAFSMATAPTNATAHRRAGTVKSTPPPEDKHS YTAKYYDKDIYFNIYEGRNSTPRRRTLSEIISKFSTSEMLSLKRVKAFVP VDENPTTTLEDIADILNYAVCDDNSCGCTIETQARIMFGDIIICVPLSAD NKGVRNFKDRIMPKGLSQILSSSLGLHLSLLYGAFGSNYNSLAYMRRLKP LTAMTAIRFCPMTTKLELRQNYKVKETLCELIVSIEILKIRNNGGQTMKT LTSFAIVRKDNDGQDWETCTRFAPVNIEDILRYKRVANDTCCRHRDVQHG RRTLESSNSWTQTQYFEPWQDIVDVYVPINDTHCPNDSYVVFETLQGFEW CSRLNKNETKNYLSSVLGFRNALFETEELMETIAMRLASQILSMVGQQGT TIRDIDPAIVSALWHSLPEN
Database link: Q69572
- T-cell line HSB-2 cells infected with HHV-6A; 293T cells transfected with HHV6 U100 expression plasmid.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 6
Constituents: 50% Glycerol (glycerin, glycerine), 50% PBS
Concentration information loading...
Purification notesab179902 is produced in serum-free medium and purified by proprietary chromatography procedures under mild conditions. ab179902 is 90~95% pure by SDS-PAGE and is filter-sterilised.
Our Abpromise guarantee covers the use of ab179902 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.
|WB||1/500 - 1/1000.|
|ICC/IF||1/100 - 1/3200.|
|IP||Use at an assay dependent concentration.
RelevanceHuman herpesvirus 6 (HHV-6) is the common collective name for Human herpesvirus 6A (HHV-6A) and Human herpesvirus 6B (HHV-6B). These closely related viruses are two of the nine herpesviruses known to have humans as their primary host. HHV-6A and HHV-6B are double stranded DNA viruses within the betaherpesvirinae subfamily and of the genus Roseolovirus. HHV-6A and HHV-6B infects almost all of the human populations tested. The overall nucleotide sequence identity between HHV-6A and HHV-6B is 90% and they are now classified as distinct species. HHV-6A has been described as more neurovirulent, and as such is more frequently found in patients with neuroinflammatory diseases such as multiple sclerosis. HHV-6B primary infection is the cause of the common childhood illness exanthem subitum (also known as roseola infantum or sixth disease). Additionally, HHV-6B reactivation is common in transplant recipients, which can cause several clinical manifestations such as encephalitis, bone marrow suppression and pneumonitis. gQ1 encoded by the U100 gene of HHV6 is glycoprotein, complexes with gH, gL and gQ2 to form HHV6A ligand to CD46 receptor and HHV6B ligand to CD134 receptor. It is expressed in two different forms: an 80-kDa form (gQ1-80K) and a 74-kDa form (gQ1-74K) - only gQ1-80K, but not gQ1-74K, forms the ligand complex with gQ2, gH, and gL. It associates with lipid rafts.
All lanes : Anti-HHV6AgQ1 antibody  - BSA and Azide free (ab179902)
Lane 1 : Non-treated
Lane 2 : Treated with end-glycosilase
Lane 3 : Treated with peptide N -glycosidase
gQ1 is detected as two glycoproteins with 80 kDa and 74 kDa molecular masses
All lanes : Anti-HHV6AgQ1 antibody  - BSA and Azide free (ab179902)
Lane 1 : HSB-2 cells
Lane 2 : HSB-2 cells infected with HHV-6A GS strain
Immunofluorescent analysis of HHV6A-infected HSB-2 cells labeling HHV6 U100 using FITC-conjugated ab179902 at 1/200 dilution. The infected cells were harvested 3 days postinfection, fixed in cold acetone.
293T cells were transfected with gQ1 expression plasmid. The cells were harvested on second day postinfection, fixed with 4% PFA, permeabilized with 0.1% Triton-X100, incubated with anti-gQ1 antibody (119) and then with FITC-conjugated anti-mouse IgG antibody.Histograms show fluorescence intensities measured in arbitary units on a log scale (x axis) and relative cell numbers on a linear scale (y axis).Black line is control and red line is gQ1 introduced cells.
ab179902 has been referenced in 7 publications.
- Tang H et al. Human herpesvirus 6 glycoprotein complex formation is required for folding and trafficking of the gH/gL/gQ1/gQ2 complex and its cellular receptor binding. J Virol 85:11121-30 (2011). PubMed: 21849437
- Tang H et al. Human herpesvirus 6 encoded glycoprotein Q1 gene is essential for virus growth. Virology 407:360-7 (2010). PubMed: 20863544
- Huang H et al. Human herpesvirus 6 envelope cholesterol is required for virus entry. J Gen Virol 87:277-85 (2006). PubMed: 16432012
- Mori Y et al. Discovery of a second form of tripartite complex containing gH-gL of human herpesvirus 6 and observations on CD46. J Virol 78:4609-16 (2004). PubMed: 15078943
- Akkapaiboon P et al. Intracellular processing of human herpesvirus 6 glycoproteins Q1 and Q2 into tetrameric complexes expressed on the viral envelope. J Virol 78:7969-83 (2004). PubMed: 15254169
- Mori Y et al. The human herpesvirus 6 U100 gene product is the third component of the gH-gL glycoprotein complex on the viral envelope. J Virol 77:2452-8 (2003). PubMed: 12551983
- Mori Y et al. Human herpesvirus 6 variant A glycoprotein H-glycoprotein L-glycoprotein Q complex associates with human CD46. J Virol 77:4992-9 (2003). PubMed: 12663806