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    hhv8-antibody-hhv83606-ab267354.pdf

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Microbiology Organism Virus DNA Virus double stranded DNA Virus HHV
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Anti-HHV8 antibody [HHV8/3606] (ab267354)

  • Datasheet
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HHV8 antibody [HHV8/3606] (ab267354)

    Key features and details

    • Mouse monoclonal [HHV8/3606] to HHV8
    • Suitable for: IHC-P
    • Reacts with: Human herpesvirus
    • Isotype: IgG1

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    Overview

    • Product name

      Anti-HHV8 antibody [HHV8/3606]
      See all HHV8 primary antibodies
    • Description

      Mouse monoclonal [HHV8/3606] to HHV8
    • Host species

      Mouse
    • Tested applications

      Suitable for: IHC-Pmore details
    • Species reactivity

      Reacts with: Human herpesvirus
    • Immunogen

      Recombinant full length protein corresponding to Human herpesvirus HHV8. Recombinant protein corresponding to the latent nuclear antigen 1 molecule (product of the viral gene orf 73) of HHV8.

    • Positive control

      • IHC-P: Human Kaposi's sarcoma tissue.
    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.2
      Preservative: 0.05% Sodium azide
      Constituents: PBS, 0.05% BSA
    • Concentration information loading...
    • Purity

      Protein A/G purified
    • Purification notes

      Purified from bioreactor concentrate.
    • Clonality

      Monoclonal
    • Clone number

      HHV8/3606
    • Isotype

      IgG1
    • Light chain type

      kappa
    • Research areas

      • Microbiology
      • Organism
      • Virus
      • DNA Virus
      • double stranded DNA Virus
      • HHV

    Associated products

    • Alternative Versions

      • Anti-HHV8 antibody [HHV8/3606] - BSA and Azide free (ab267355)
    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab267354 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IHC-P
    Use a concentration of 1 - 2 µg/ml.

    Primary incucubation for 30 minutes at room temperature.

    Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes.

    Notes
    IHC-P
    Use a concentration of 1 - 2 µg/ml.

    Primary incucubation for 30 minutes at room temperature.

    Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes.

    Target

    • Relevance

      HHV8 has been found to be associated with three different diseases observed in AIDS patients; kaposi's sarcoma, primary effusion lymphoma (which is a rare type of non-Hodgkin lymphoma affecting the body cavities) and multicentric Castleman's disease. To date there is much evidence to support a direct role for HHV8 in kaposi's sarcoma.
    • Database links

      • Entrez Gene: 935114 Human herpesvirus
      • SwissProt: Q76SB0 Human herpesvirus
      • Alternative names

        • HHV 8 antibody
        • Human Herpes Virus 8 antibody
        • Human herpesvirus 8 antibody
        • Kaposi's sarcoma associated herpes virus antibody
        • KSHV antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HHV8 antibody [HHV8/3606] (ab267354)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HHV8 antibody [HHV8/3606] (ab267354)

        Formalin-fixed, paraffin-embedded human Kaposi's sarcoma tissue stained for HHV8 using ab267354 at 2 μg/ml in immunohistochemical analysis.

        Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes.

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet download

        Download

      References (0)

      Publishing research using ab267354? Please let us know so that we can cite the reference in this datasheet.

      ab267354 has not yet been referenced specifically in any publications.

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