Product nameAnti-HIBADH antibody [EPR12519(B)]
See all HIBADH primary antibodies
DescriptionRabbit monoclonal [EPR12519(B)] to HIBADH
Tested applicationsSuitable for: IHC-P, WB, ICC/IF, IP, Flow Cytmore details
Species reactivityReacts with: Human
Predicted to work with: Cow, OrangutanDoes not react with: Mouse, Rat
Synthetic peptide within Human HIBADH aa 50-150 (Cysteine residue). The exact sequence is proprietary.
Database link: P31937
- Human fetal liver, fetal heart and fetal kidney tissue lysate and HepG2 cell lysate; Human kidney and liver tissue; HepG2 cells
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab175203 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/1000 - 1/10000. Predicted molecular weight: 35 kDa.|
|ICC/IF||1/100 - 1/250.|
|IP||1/10 - 1/100.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
- Information by UniProt
- 3 hydroxy 2 methylpropanoate:NAD(+) oxidoreductase antibody
- 3 hydroxyisobutyrate dehydrogenase antibody
- 3 hydroxyisobutyrate dehydrogenase mitochondrial antibody
All lanes : Anti-HIBADH antibody [EPR12519(B)] (ab175203) at 1/1000 dilution
Lane 1 : Human fetal liver tissue lysate
Lane 2 : Human fetal heart tissue lysate
Lane 3 : HepG2 cell lysate
Lane 4 : Human fetal kidney tissue lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 35 kDa
Immunofluorescent staining of HepG2 cells labeling HIBADH with ab175203 at 1/100 dilution (red). DAPI nuclear staining (blue).
Immunohistochemical analysis of paraffin embedded Human kidney tissue labeling HIBADH with ab175203 at 1/50 dilution.
Immunohistochemical analysis of paraffin embedded Human liver tissue labeling HIBADH with ab175203 at 1/50 dilution.
Flow cytometric analysis of permeabilized HepG2 cells labeling HIBADH with ab175203 at 1/10 dilution (red) compared to a Rabbit IgG (green).
Western blot analysis on immunoprecipitation pellet from HepG2 cell lysate labeling HIBADH with ab175203 at 1/10 dilution.
ab175203 has not yet been referenced specifically in any publications.