Overview

  • Product name
    Anti-HIBADH antibody [EPR12519(B)]
    See all HIBADH primary antibodies
  • Description
    Rabbit monoclonal [EPR12519(B)] to HIBADH
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IF, IP, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cow, OrangutanDoes not react with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human HIBADH aa 50-150 (Cysteine residue). The exact sequence is proprietary.
    Database link: P31937

  • Positive control
    • Human fetal liver, fetal heart and fetal kidney tissue lysate and HepG2 cell lysate; Human kidney and liver tissue; HepG2 cells
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab175203 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000 - 1/10000. Predicted molecular weight: 35 kDa.
ICC/IF 1/100 - 1/250.
IP 1/10 - 1/100.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

Images

  • Immunofluorescent staining of HepG2 cells labeling HIBADH with ab175203 at 1/100 dilution (red). DAPI nuclear staining (blue).

  • All lanes : Anti-HIBADH antibody [EPR12519(B)] (ab175203) at 1/1000 dilution

    Lane 1 : Human fetal liver tissue lysate
    Lane 2 : Human fetal heart tissue lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : Human fetal kidney tissue lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 35 kDa

  • Immunohistochemical analysis of paraffin embedded Human kidney tissue labeling HIBADH with ab175203 at 1/50 dilution.

  • Immunohistochemical analysis of paraffin embedded Human liver tissue labeling HIBADH with ab175203 at 1/50 dilution.

  • Flow cytometric analysis of permeabilized HepG2 cells labeling HIBADH with ab175203 at 1/10 dilution (red) compared to a Rabbit IgG (green).

  • Western blot analysis on immunoprecipitation pellet from HepG2 cell lysate labeling HIBADH with ab175203 at 1/10 dilution.

References

ab175203 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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