Recombinant
RabMAb

Recombinant Anti-HIBADH antibody [EPR12525(B)] (ab172955)

Overview

  • Product name

    Anti-HIBADH antibody [EPR12525(B)]
    See all HIBADH primary antibodies
  • Description

    Rabbit monoclonal [EPR12525(B)] to HIBADH
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, Flow Cyt, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human HIBADH aa 300 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: P31937

  • Positive control

    • Fetal kidney, Fetal liver, 293T, MCF-7 and HepG2 lysates; Human breast carcinoma and liver tissues; HepG2 cells; 293T cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab172955 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 35 kDa.
IHC-P 1/100 - 1/250.
ICC/IF 1/250 - 1/500.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

IP 1/100 - 1/500.

Target

Images

  • All lanes : Anti-HIBADH antibody [EPR12525(B)] (ab172955) at 1/10000 dilution

    Lane 1 : Fetal kidney lysate
    Lane 2 : Fetal liver lysate
    Lane 3 : 293T lysate
    Lane 4 : MCF-7 lysate
    Lane 5 : HepG2 lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 35 kDa

  • Immunofluorescent analysis of HepG2 cells labeling HIBADH with ab172955 at 1/250 dilution.

  • Western blot analysis on immunoprecipitation pellet from HepG2 cell lysate using ab172955 at a 1/100 dilution.

  • Flow cytometric analysis of permeabilized 293T cells labeling HIBADH with ab172955 at 1/10 dilution (red) compared to a rabbit IgG negative control (green).

  • Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling HIBADH with ab172955 at 1/100 dilution.

  • Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling HIBADH with ab172955 at 1/100 dilution.

References

ab172955 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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