Product nameAnti-HIBADH antibody [EPR12525(B)]
See all HIBADH primary antibodies
DescriptionRabbit monoclonal [EPR12525(B)] to HIBADH
Tested applicationsSuitable for: WB, IHC-P, ICC/IF, Flow Cyt, IPmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human HIBADH aa 300 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: P31937
- Fetal kidney, Fetal liver, 293T, MCF-7 and HepG2 lysates; Human breast carcinoma and liver tissues; HepG2 cells; 293T cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab172955 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Predicted molecular weight: 35 kDa.|
|IHC-P||1/100 - 1/250.|
|ICC/IF||1/250 - 1/500.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|IP||1/100 - 1/500.|
- Information by UniProt
- 3 hydroxy 2 methylpropanoate:NAD(+) oxidoreductase antibody
- 3 hydroxyisobutyrate dehydrogenase antibody
- 3 hydroxyisobutyrate dehydrogenase mitochondrial antibody
All lanes : Anti-HIBADH antibody [EPR12525(B)] (ab172955) at 1/10000 dilution
Lane 1 : Fetal kidney lysate
Lane 2 : Fetal liver lysate
Lane 3 : 293T lysate
Lane 4 : MCF-7 lysate
Lane 5 : HepG2 lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 35 kDa
Immunofluorescent analysis of HepG2 cells labeling HIBADH with ab172955 at 1/250 dilution.
Western blot analysis on immunoprecipitation pellet from HepG2 cell lysate using ab172955 at a 1/100 dilution.
Flow cytometric analysis of permeabilized 293T cells labeling HIBADH with ab172955 at 1/10 dilution (red) compared to a rabbit IgG negative control (green).
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling HIBADH with ab172955 at 1/100 dilution.
Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling HIBADH with ab172955 at 1/100 dilution.
ab172955 has not yet been referenced specifically in any publications.