Anti-HIBCH antibody (ab153826)
Key features and details
- Rabbit polyclonal to HIBCH
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-HIBCH antibody -
Description
Rabbit polyclonal to HIBCH -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 1-288 of Human HIBCH (UniProt: Q6NVY1).
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Positive control
- 293T, HepG2, Molt4 whole cell lysates; A431 cells; Hepatoma tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab153826 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 43 kDa.
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IHC-P |
1/100 - 1/1000.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 43 kDa. |
IHC-P
1/100 - 1/1000. |
ICC/IF
1/100 - 1/1000. |
Target
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Function
Hydrolyzes 3-hydroxyisobutyryl-CoA (HIBYL-CoA), a saline catabolite. Has high activity toward isobutyryl-CoA. Could be an isobutyryl-CoA dehydrogenase that functions in valine catabolism. Also hydrolyzes 3-hydroxypropanoyl-CoA. -
Tissue specificity
Highly expressed in liver and kidney, also detected in heart, muscle and brain (at protein level). Not detected in lung. -
Pathway
Amino-acid degradation; L-valine degradation. -
Involvement in disease
Defects in HIBCH are the cause of HIBCH deficiency (HIBCHD) [MIM:250620]; also known as deficiency of beta-hydroxyisobutyryl CoA deacylase or methacrylic aciduria. The enzyme defect results in accumulation of methacrylyl-CoA, a highly reactive compound, which readily undergoes addition reactions with free sulfhydryl groups. Affected individuals showed delayed development of motor skills, hypotonia, initial poor feeding, and a deterioration in neurological function during first stages of life. -
Sequence similarities
Belongs to the enoyl-CoA hydratase/isomerase family. -
Cellular localization
Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 26275 Human
- Omim: 610690 Human
- SwissProt: Q6NVY1 Human
- Unigene: 656685 Human
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Alternative names
- 3 hydroxyisobutyryl Coenzyme A hydrolase antibody
- 3 hydroxyisobutyryl Coenzyme A hydrolase, mitochondrial antibody
- 3-hydroxyisobutyryl-CoA hydrolase antibody
see all
Images
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All lanes : Anti-HIBCH antibody (ab153826) at 1/1000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : HepG2 whole cell lysate
Lane 3 : Molt4 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 43 kDa
10% SDS PAGE -
Immunofluorescence analysis of methanol fixed A431 cells labeling HIBCH with ab153826 at 1/200. Lower panel shows costaining with Hoechst 33342.
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Immunohistochemical analysis of paraffin embedded Human hepatoma tissue labeling HIBCH with ab153826 at 1/500.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab153826 has been referenced in 1 publication.
- Shan Y et al. Targeting HIBCH to reprogram valine metabolism for the treatment of colorectal cancer. Cell Death Dis 10:618 (2019). PubMed: 31409769